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Hydrogen Peroxide and Cell Signaling, Part C, Volume 528 [Kõva köide]

Volume editor (Department of Molecular Pharmacology and Toxicology, School of Pharmaceutical Sciences, University of Southern California, USA), Volume editor (Pharmacology & Pharmaceutical Sciences, School of Pharmacy, University of Southern California, USA)
  • Formaat: Hardback, 384 pages, kõrgus x laius: 229x152 mm, kaal: 760 g
  • Sari: Methods in Enzymology
  • Ilmumisaeg: 03-Sep-2013
  • Kirjastus: Academic Press Inc
  • ISBN-10: 0124058817
  • ISBN-13: 9780124058811
Teised raamatud teemal:
Hydrogen Peroxide and Cell Signaling, Part C, Volume 528Suurem pilt
  • Formaat: Hardback, 384 pages, kõrgus x laius: 229x152 mm, kaal: 760 g
  • Sari: Methods in Enzymology
  • Ilmumisaeg: 03-Sep-2013
  • Kirjastus: Academic Press Inc
  • ISBN-10: 0124058817
  • ISBN-13: 9780124058811
Teised raamatud teemal:
Püsilink: https://www.kriso.ee/db/9780124058811.html
Märksõnad:

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This is the third of three volumes on hydrogen peroxide and cell signaling, and includes chapters on such topics as the biological chemistry of hydrogen peroxide, reactive oxygen species in the activation of MAP kinases, and investigating the role of reactive oxygen species in regulating autophagy.

  • Continues the legacy of this premier serial with quality chapters authored by leaders in the field
  • Covers hydrogen peroxide and cell signaling
  • Contains chapters on such topics as the biological chemistry of hydrogen peroxide, reactive oxygen species in the activation of MAP kinases, and investigating the role of reactive oxygen species in regulating autophagy

Muu info

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This is the third of three volumes on hydrogen peroxide and cell signaling.
Contributors xi
Preface xv
Volumes in Series xvii
Section I H2O2 Regulation of Cell Signaling
1 The Biological Chemistry of Hydrogen Peroxide
3(24)
Christine C. Winterbourn
1 Introduction
4(1)
2 Chemical Properties
4(7)
3 Antioxidant Defenses Against H2O2
11(2)
4 Kinetics and Identification of Biological Targets for H2O2
13(2)
5 Transmission of Redox Signals Initiated by H2O2
15(2)
6 Diffusion Distances and Compartmentalization
17(2)
7 Biological Detection of H2O2
19(1)
8 Conclusion
20(7)
References
21(6)
2 Reactive Oxygen Species in the Activation of MAP Kinases
27(22)
Yong Son
Sangduck Kim
Hun-Taeg Chung
Hyun-Ock Pae
1 Introduction
28(1)
2 Reactive Oxygen Species
29(4)
3 Mitogen-Activated Protein Kinases
33(6)
4 Roles of ROS in MAPK Activation
39(5)
5 Summary
44(5)
Acknowledgment
45(1)
References
45(4)
3 Hydrogen Peroxide Signaling Mediator in the Activation of p38 MAPK in Vascular Endothelial Cells
49(12)
Rosa Breton-Romero
Santiago Lamas
1 Introduction
50(1)
2 Materials and Methods
51(10)
References
58(3)
4 In Vivo Imaging of Nitric Oxide and Hydrogen Peroxide in Cardiac Myocytes
61(18)
Juliano L. Sartoretto
Hermann Kalwa
Natalia Romero
Thomas Michel
1 Introduction
62(1)
2 Isolation and Culture of Adult Mouse Ventricular Cardiac Myocytes
63(2)
3 Live Cell Imaging of Cardiac Myocytes
65(1)
4 Imaging Intracellular NO with Cu2(FL2E) Dye
66(2)
5 Production and In Vivo Expression of Lentivirus Expressing the HyPer2 H2O2 Biosensor
68(8)
6 Imaging Intracellular H2O2 in Cardiac Myocytes and Endothelial Cells Expressing HyPer2
76(3)
Acknowledgments
77(1)
References
77(2)
5 Methods for Studying Oxidative Regulation of Protein Kinase C
79(20)
Rayudu Gopalakrishna
Thomas H. McNeill
Albert A. Elhiani
Usha Gundimeda
1 Introduction
80(3)
2 Materials
83(2)
3 Direct Oxidative Modification of PKC Isoenzymes by H2O2
85(3)
4 Indirect Cellular Regulation of PKC Isoenzymes by Sublethal Levels of H2O2
88(2)
5 H2O2-Induced Signaling in GTPP-Induced Preconditioning for Cerebral Ischemia
90(5)
6 Summary
95(4)
Acknowledgments
95(1)
References
95(4)
6 p66Shc, Mitochondria, and the Generation of Reactive Oxygen Species
99(12)
Mirella Trinei
Enrica Migliaccio
Paolo Bernardi
Francesco Paolucci
Piergiuseppe Pelicci
Marco Giorgio
1 Introduction
100(1)
2 The P66 Gene and Protein
100(1)
3 The Mitochondrial Function of p66Shc
101(1)
4 Preparation of Recombinant p66Shc Protein
102(2)
5 Mitochondrial Swelling Assay
104(1)
6 Mitochondrial ROS Formation by p66Shc
105(2)
7 Conclusions: Role of p66Shc ROS
107(4)
Acknowledgments
108(1)
References
108(3)
7 Detecting Disulfide-Bound Complexes and the Oxidative Regulation of Cyclic Nucleotide-Dependent Protein Kinases by H2O2
111(18)
Joseph R. Burgoyne
Philip Eaton
1 Introduction
112(9)
2 Experimental Considerations and Procedures
121(3)
3 Summary
124(5)
Acknowledgments
125(1)
References
125(4)
8 Redox Regulation of Protein Tyrosine Phosphatases: Methods for Kinetic Analysis of Covalent Enzyme Inactivation
129(28)
Zachary D. Parsons
Kent S. Gates
1 Introduction
130(3)
2 Rate Expressions Describing Covalent Enzyme Inactivation
133(1)
3 Ensuring That the Enzyme Activity Assay Accurately Reflects the Amount of Active Enzyme
134(5)
4 Assays for Time-Dependent Inactivation of PTPs
139(3)
5 Analysis of the Kinetic Data
142(8)
6 Obtaining an Inactivation Rate Constant from the Data
150(2)
7 Summary
152(5)
References
152(5)
Section II H2O2 in the Redox Regulation of Transcription and Cell-Surface Receptors
9 Activation of Nrf2 by H2O2: De Novo Synthesis Versus Nuclear Translocation
157(16)
Goncalo Covas
H. Susana Marinho
Luisa Cyrne
Fernando Antunes
1 Introduction
158(1)
2 Experimental Conditions and Considerations
159(4)
3 Pilot Experiments
163(1)
4 Experimental H2O2 Exposure
164(2)
5 Data Handling and Analysis
166(4)
6 Summary
170(3)
Acknowledgments
170(1)
References
170(3)
10 H2O2 in the Induction of NF-κB-Dependent Selective Gene Expression
173(16)
Luisa Cyrne
Virginia Oliveira-Marques
H. Susana Marinho
Fernando Antunes
1 Introduction
174(1)
2 Experimental Components and Considerations
175(4)
3 Pilot Experiments
179(2)
4 Steady-State Titration Experiments
181(1)
5 NF-κB Family Protein Levels
182(2)
6 NF-κB-Dependent Gene Expression
184(3)
7 Summary
187(2)
Acknowledgments
187(1)
References
187(2)
11 Detection of H2O2-Mediated Phosphorylation of Kinase-Inactive PDGFRα
189(8)
Hetian Lei
Andrius Kazlauskas
1 Construction of Kinase-Dead PDGFRα
190(1)
2 Characterization of the Kinase-Inactive Receptor
191(1)
3 Detection of H2O2-Mediated Phosphorylation of Kinase-Inactive PDGFRα
192(2)
4 Implication
194(3)
Acknowledgment
194(1)
References
194(3)
Section III H2O2 and Regulation of Cellular Processes
12 Genetic Modifier Screens to Identify Components of a Redox-Regulated Cell Adhesion and Migration Pathway
197(20)
Thomas Ryan Hurd
Michelle Gail Leblanc
Leonard Nathaniel Jones
Matthew DeGennaro
Ruth Lehmann
1 Introduction
198(1)
2 Mutations in a D. melanogaster Gene Encoding a Peroxiredoxin Cause Germ Cell Adhesion and Migration Defects
199(1)
3 Dominant Modifier Screens
200(1)
4 Conducting a Dominant Modifier Screen to Identify Missing Components of a Redox-Regulated Germ Cell Migration Pathway
201(10)
5 Limitations to Dominant Modifiers Screens
211(1)
6 Concluding Remarks
212(5)
Acknowledgments
213(1)
References
213(4)
13 Investigating the Role of Reactive Oxygen Species in Regulating Autophagy
217(20)
Spencer B. Gibson
1 Introduction
218(1)
2 Regulation of Autophagy
218(2)
3 ROS and Autophagy
220(1)
4 Mechanisms for ROS Regulation of Autophagy
221(4)
5 Methods for the Detection of Autophagy
225(4)
6 Consideration When Using Oxidative Stress and Detecting ROS Under Autophagy Conditions
229(2)
7 Conclusions
231(6)
Acknowledgment
231(1)
References
232(5)
14 H2O2: A Chemoattractant?
237(20)
Balazs Enyedi
Philipp Niethammer
1 Introduction
238(4)
2 The Zebrafish Tail Fin Wounding Assay
242(4)
3 Measuring H2O2 Signals in Zebrafish
246(2)
4 Imaging H2O2 Production by Wide-Field Microscopy
248(1)
5 Imaging H2O2 Production by Confocal Microscopy
249(8)
Acknowledgments
253(1)
References
253(4)
15 Measuring Mitochondrial Uncoupling Protein-2 Level and Activity in Insulinoma Cells
257(12)
Jonathan Barlow
Verena Hirschberg
Martin D. Brand
Charles Affourtit
1 Introduction
258(1)
2 Tissue Culture
258(1)
3 UCP2 Protein Detection
259(1)
4 UCP2 Protein Knockdown
260(1)
5 UCP2 Activity
261(8)
Acknowledgments
267(1)
References
267(2)
16 Effects of H2O2 on Insulin Signaling the Glucose Transport System in Mammalian Skeletal Muscle
269(10)
Erik J. Henriksen
1 Introduction
270(1)
2 In Vitro Exposure to H2O2
271(3)
3 Effects of H2O2 on the Glucose Transport System in Isolated Skeletal Muscle
274(1)
4 Summary
275(4)
References
276(3)
17 Monitoring of Hydrogen Peroxide and Other Reactive Oxygen and Nitrogen Species Generated by Skeletal Muscle
279(18)
Malcolm J. Jackson
1 Introduction
280(4)
2 Monitoring Extracellular ROS Using Microdialysis Techniques
284(6)
3 Assessment of Intracellular ROS Activities
290(6)
4 Concluding Remarks
296(1)
Acknowledgments 297(1)
References 297(4)
Author Index 301(22)
Subject Index 323
ENRIQUE CADENAS, MD, PhD, received his PhD in biochemistry from the University of Buenos Aires, School of Medicine. He is professor of pharmacology and pharmaceutical sciences at the University of Southern California School of Pharmacy and of biochemistry and molecular biology at the University of Southern California Keck School of Medicine, and doctor honoris causa (medicine) at the University of Linköping, Sweden. Cadenas was president of the Society for Free Radical Research International (SFRRI) and is fellow of the Society for Free Radical Biology & Medicine. He served the scientific community by participating on NIH study sections (2002-2006; chair 2006-2008). His research interests include energy and redox metabolism in brain aging and the coordinated inflammatory-metabolic responses in brain and neurodegenerative diseases. Lester Packer received a PhD in Microbiology and Biochemistry in 1956 from Yale University. In 1961, he joined the University of California at Berkeley serving as Professor of Cell and Molecular Biology until 2000, and then was appointed Adjunct Professor, Pharmacology and Pharmaceutical Sciences, School of Pharmacy at the University of Southern California. Dr Packer received numerous distinctions including three honorary doctoral degrees, several distinguished Professor appointments. He was awarded Chevalier de lOrdre National du Merite (Knight of the French National Order of Merit) and later promoted to the rank of Officier. He served as President of the Society for Free Radical Research International (SFRRI), founder and Honorary President of the Oxygen Club of California. He has edited numerous books and published research; some of the most cited articles have become classics in the field of free radical biology: Dr Packer is a member of many professional societies and editorial boards. His research elucidated - the Antioxidant Network concept. Exogenous lipoic acid was discovered to be one of the most potent natural antioxidants and placed as the ultimate reductant or in the pecking order of the Antioxidant Network” regenerating vitamins C and E and stimulating glutathione synthesis, thereby improving the overall cellular antioxidant defense. The Antioxidant Network is a concept addressing the cells redox status. He established a world-wide network of research programs by supporting and co-organizing conferences on free radical research and redox biology in Asia, Europe, and America.