Immuno-oncology and immunotherapy, Part D, Volume 196 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics, including Investigating the endothelial-immune axis in cancer, Establishing Tyro3, Axl, and Mertk Chinese Hamster Ovary (CHO) Reporter Cell Lines for Cancer Immunology and Therapeutic Applications, Amino acid analog-based labelling of the immunomodulatory cancer secretome, Human and mouse iNKT cell expansion and engineering with viral vectors, Next-generation deconvolution of the tumor microenvironment with omnideconv, and much more.
Additional sections cover Comprehensive prediction of tumor neoantigens with NextNEOpi, Ex vivo profiling of organotypic tumor spheroids in 3D microfluidic culture, A flow cytometry-based assay for evaluation of T cell activation and degranulation in coculture systems, An adenosine production assay suitable for 2D and 3D cancer cell culture, Determination of lactate in the tumor interstitial fluid, Combined in vitro differentiation and cell sorting-based isolation of highly pure mouse bone marrow-derived basophils, Organ-on-Chip immunostaining method for three-dimensional identification and study of immune cells responding to drug-treated tumor cells, and much more.
1. Investigating the endothelial-immune axis in cancer
Gabriele Bergers and Kathryn A Kathryn A. Jacobs
2. Establishing Tyro3, Axl, and Mertk Chinese Hamster Ovary (CHO) Reporter
Cell Lines for Cancer Immunology and Therapeutic Applications
Raymond Birge
3. Amino acid analog-based labelling of the immunomodulatory cancer
secretome
Karin E. de Visser, Annemieke C. Bouwman, Antoinette van Weverwijk, Onno B.
Bleijerveld, Liesbeth Hoekman, Bob J. Ignacio and Kimberly M. Bonger
4. Human and mouse iNKT cell expansion and engineering with viral vectors
Gloria Delfanti, Paolo Dellabona, Giulia Casorati and Michela Consonni
5. Next-generation deconvolution of the tumor microenvironment with
omnideconv
Francesca Finotello, Markus List, Alexander Dietrich and Lorenzo Merottto
6. Comprehensive prediction of tumor neoantigens with NextNEOpi
Francesca Finotello, Dietmar Rieder and Markus Ausserhofer
7. Ex vivo profiling of organotypic tumor spheroids in 3D microfluidic
culture
Russell W. Jenkins
8. A flow cytometry-based assay for evaluation of T cell activation and
degranulation in coculture systems
Andreas Lunqdvist
9. An adenosine production assay suitable for 2D and 3D cancer cell culture
Andreas Lunqdvist and Apple Tay Hui Min
10. Determination of lactate in the tumor interstitial fluid
Santos Manes, Ainhoa Ruíz-Iglesias, Angel García-Aldea, Elena Nonnast and
Rosa M. Peregil
11. Combined in vitro differentiation and cell sorting-based isolation of
highly pure mouse bone marrow-derived basophils.
Fabrizio Mattei and Giovanna Schiavoni
12. Organ-on-Chip immunostaining method for three-dimensional identification
and study of immune cells responding to drug-treated tumor cells
Fabrizio Mattei, Giovanna Schiavoni, Francesco Noto, Adele De Ninno, Mario
Falchi and Luca Businaro
13. Evaluating transcriptional TME responses to immunotherapy using
microarray analysis
Karen Mossman
14. Expansion and characterization of human peripheral blood derived NK cells
for functional studies on tumour organoids
Julian Pardo Jimeno Sr. and ARIEL RAMIREZ LABRADA
15. Inflammatory Neovascularization Analysis In Vivo in Matrigel Implants
with Isolated IDVCs (IDO1-Dependent Vascularizing Cells)
Alexander J. Muller, Souvik Dey, Arpita Mondal, James DuHadaway and Shih-Chun
Shen
16. Phenotypic assessment of dendritic cell maturation by cost-effective
custom ELISA assays
Peng LIU, Guido Kroemer, Oliver Kepp and Liwei Zhao
17. Pharmacological screening to identify potential regulators of antigen
presentation by dendritic cells
Peng LIU, Liwei Zhao, Guido Kroemer, Oliver Kepp, Yuhong Pan and Shuai Zhang
Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology.
Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals: OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience. Norma received her masters degree in health biology from Paris Saclay. In 2013, she joined the laboratory of Dr. Guido Kroemer at the Centre de Recherche des Cordeliers (Paris, France) and at Gustave Roussy (Villejuif, France), the largest center for oncological patients in Europe. She worked on several projects linked to immunosurveillance, culminating with her obtaining her PhD in 2017 with an original work on Immunogenic stress and death of cancer cells: Contribution of antigenicity vs adjuvanticity to immunosurveillance”. She then moved to Weill Cornell Medicine to join the program in radiation and immunity under the mentorship of Dr. Galluzzi. Her current research is focused on investigating resistance to immunotherapy in a mouse model of HR+ breast cancer.
Maud Charpentier received her M.Sc. in Cellular Biology and her Ph.D. in Immunology from the University of Nantes, France. She chose to pursue an academic career and continued her postdoctoral training in the United States. She joined the Department of Radiation Oncology at Weill Cornell Medicine under the mentorship of Dr. Sandra Demaria. Maud has a long-standing interest in the anti-tumor immune response and its role in controlling cancer progression and treatment outcomes. Her research focuses on understanding the synergy between radiation therapy and immunotherapy in solid tumors, with the aim of overcoming resistance to treatment and developing innovative therapeutic approaches in preclinical models.
