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Immunology: Overview and Laboratory Manual Second Edition 2026 [Kõva köide]

  • Formaat: Hardback, 589 pages, kõrgus x laius: 235x155 mm, 42 Illustrations, color; 38 Illustrations, black and white
  • Ilmumisaeg: 30-May-2026
  • Kirjastus: Springer Nature Switzerland AG
  • ISBN-10: 3032199972
  • ISBN-13: 9783032199973
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  • Formaat: Hardback, 589 pages, kõrgus x laius: 235x155 mm, 42 Illustrations, color; 38 Illustrations, black and white
  • Ilmumisaeg: 30-May-2026
  • Kirjastus: Springer Nature Switzerland AG
  • ISBN-10: 3032199972
  • ISBN-13: 9783032199973
Teised raamatud teemal:
A second edition, two-in-one text to be utilized in providing students with an overview of immunology, as well as a lab manual complete with current standard exercises. 



Section I of this book provides an overview of the immune system and immunity, and includes review questions, problem sets, case studies, inquiry-based questions, and more to provide students with a strong foundation in the field. New chapters on trained immunity, oral immunity, mucosal immunity, chimeric antigen receptor (CAR) T cells, and immune checkpoint inhibitors have been added to the second edition. Section II consists of thirty-seven lab exercises focused on key concepts in immunology, such as antibody production, cell separation, cell function, immunoassays, Th1/Th2 cytokine detection, chromatin immunoprecipitation (ChIP), nitric oxide detection, cell and tissue culture methods, and cell and molecular biology techniques. Appendices include safety information, suggested links and readings, and standard discipline processes, protocols, and instructions.
Part I: Overview of Immunity.- Ch 1: Immunity.- Ch 2: Components of
immunity.- Ch 3: Hematopoiesis.- Ch 4: The Immune system: pathogen sensing
and detection.- Ch 5: Lymphoid organs.- Ch 6: Innate and Adaptive immunity.-
Ch 7: Leukocyte homing, migration and recirculation.- Ch 8: Apoptosis.- Ch 9:
Antigens.- Ch 10: B cell development, activation and immunoglobulin
structure.- Ch 11: Immunoglobulin and T cell receptor gene rearrangements.-
Ch 12: Major histocompatibility complex proteins.- Ch 13: T cell development
and T cell receptor structure.- Ch 14: Cytokines.- Ch 15: Lymphocyte
signals.- Ch 16: Complement fixation.- Ch 17: Cell mediated immunity.- Ch 18:
Hypersensitivities.- Ch 19: Parasite Immunity.- Ch 20: Vaccines.- Ch 21:
Transplantation.- Ch 22: Immune regulation and autoimmunity.- Ch 23:
Immunodeficiencies.- Ch 24: Cancer.- Ch 25: Practice questions.- Ch 26: Study
guide for exam. Part II. Laboratory Manual.-
27. Immunology laboratory
policies.-
28. Instructions for notebook entries.-
29. Instructions for
writing lab reports.-
30. Lab report topics.-
31. Exercise 1: Using a
compound microscope.-
32. Exercise 2: Pipetting and dilution technique,
identification of mouse lymphoid organs.-
33. Exercise 3: Blood Cell
Preparation, Leukocyte differentiation and ABO Blood Typing.-
34. Exercise
4: Single Suspension of Mouse Spleen cells, Cell Viability Assays, and
Identification of specific cells using surface antigens.-
35. Exercise 5:
Isolation of mouse peritoneal macrophages: In vitro phagocytosis of bacterial
cells.-
36. Exercise 6: Clearance of bacteria from mouse blood by the RES.-
37. Exercise 7: Hemolytic Plaque assay (The Jeme Plaque Assay).- 38. EXERCISE
8: Rabbit and Mouse Immunizations- Preparation of Polyclonal antibodies and
screening assays.- 39. EXERCISE 9: Determination of Antibody Titer: Screening
Techniques.- 40. EXERCISE 10: Immunodiffusion and Immunoelectrophoresis.-
41. EXERCISE 11: Protein Affinity Purification of Rabbit IgG.- 42. EXERCISE
12: Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE).-
43. EXERCISE 13: Immunoblotting (Western Blotting).-
44. Exercise 14: Protein
Assay.-
45. Exercise 15: Enzyme-linked Immunosorbent Assay (ELISA).-
46. EXERCISE 16: Enzyme-Linked Immunospot Assay (ELISPOT).- 47. EXERCISE 17:
Immunofluorescence assay (IFA).- 48. EXERCISE 18: Flow Cytometry.-
49.EXERCISE 19: Complement Fixation Assays.-
50. Tissue Cell Culture
Techniques.-
51. EXERCISE 20: Mitogen Induced Response of
Lymphocytes:Detection of Band T cell Lymphocytes Using Immunofluorescence.-
52. Exercise 21: Induction of IL-2 Secretion from Mouse Lymphoma Cells.-
53. EXERCISE 22: Adoptive Transfer of Lymphocytes: Band T cell Cooperation
for Antibody Production.- 54. EXERCISE 23: Macrophage Migration Inhibition.-
55. EXERCISE 24: Monoclonal Antibody Preparation.- 56. EXERCISE 25:
Immunization of mice with a recombinant protein.- 57. EXERCISE 26:
Polarization of T helper cells into TH1 subset.-
58. Exercise 27:
Immunoprecipitation.- 59. EXERCISE 28: Induction of apoptosis in T
lymphocytes and detection of apoptotic markers.- 60. EXERCISE 29: Cloning and
sequencing of cDNAs of mouse variable regions of H and L chains of monoclonal
antibodies.- Appendix.- Sample Practice Questions.
Dr. Tobili Sam-Yellowe is a research scientist and educator with over 35 years experience in malaria research and teaching immunology. Dr. Sam-Yellowe's research includes Colpodella species cell biology and colpodellosis research, vaccine discovery, and research design and analysis. Dr. Sam-Yellowe is a Professor of Biology at Cleveland State University.