Lysosomes are key subcellular organelles that regulate the cell function. Many of the essential activities of the cell are dependent on lysosomes. Dysfunction is linked to multiple diseases - storage disorders, neurodegeneration, immunological diseases and cancer. This book discusses concepts and methods used to study lysosome ion and small molecule transport. The contents will not only attract accomplished investigators in need of a broad review and synthesis of this important subject but will also appeal to young investigators and trainees needing to acquire comprehensive knowledge and technical skills working with lysosomal ion channels and small molecule transporters.
Key selling features:
Summarizes the endocellular role that lysosomes play with respect to cellular waste disposalReviews essential cellular functions of lysosomesExplores how lysosome dysfunction is the cause of many metabolic disordersExamines how lysomes are involved in storage diseasesDescribes various technologies and methods used in lysosome research| Preface |
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| Editors |
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| Contributors |
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Chapter 1 Endosomal and Lysosomal Electrophysiology |
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1 | (18) |
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Chapter 2 Chloride Transport across the Lysosomal Membrane |
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19 | (26) |
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Chapter 3 Endolysosomal Patch Clamping: Approaches to Measure Vesicular Ion Channel Activities |
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45 | (32) |
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Chapter 4 TRPML Subfamily of Endolysosomal Channels: Concepts and Methods |
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77 | (26) |
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Chapter 5 Investigating the Role of Two-Pore Channel 2 (TPC2) in Zebrafish Neuromuscular Development |
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103 | (32) |
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Chapter 6 Functional Study of Lysosomal Nutrient Transporters |
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135 | (26) |
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Chapter 7 Lysosomal Vitamin B12 Trafficking |
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161 | (16) |
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Chapter 8 Detection of Lysosomal Membrane Permeabilization |
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177 | (22) |
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Chapter 9 Rapid Isolation of Lysosomes from Cultured Cells Using a Twin Strep Tag |
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199 | (10) |
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Chapter 10 A Transcriptomic Analysis and shRNA Screen for Intracellular Ion Channels and Transporters Regulating Pigmentation |
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209 | (24) |
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| Index |
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Bruno Gasnier is CNRS Research Director, Group Leader at CNRS/Paris Descartes.
Michael Xi Zhu is currently with the Department of Integrative Biology and Pharmacology at the University of Texas Health Science Center at Houston.
