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Laser Capture Microdissection: Methods and Protocols [Kõva köide]

  • Formaat: Hardback, 213 pages, kõrgus x laius: 254x178 mm, 48 Illustrations, color; 2 Illustrations, black and white
  • Sari: Methods in Molecular Biology
  • Ilmumisaeg: 03-Feb-2026
  • Kirjastus: Humana
  • ISBN-10: 1071651536
  • ISBN-13: 9781071651537
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  • Formaat: Hardback, 213 pages, kõrgus x laius: 254x178 mm, 48 Illustrations, color; 2 Illustrations, black and white
  • Sari: Methods in Molecular Biology
  • Ilmumisaeg: 03-Feb-2026
  • Kirjastus: Humana
  • ISBN-10: 1071651536
  • ISBN-13: 9781071651537
This volume details protocols that highlight the versatility of LCM across different biological contexts and analytical workflows. Chapters cover protocols on isolating cells from blood smears, live cell samples, and FFPE or frozen tissues. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.



Authoritative and practical, Laser Capture Microdissection: Methods and Protocols aims to support both new and experienced researchers in effectively applying LCM to their own biological questions, across a wide variety of systems and technical platforms.
Laser capture microdissection from blood smears.- Live cell isolation by
laser capture microdissection.- From Laser Microdissection to Spatial
Glycomics: Lectin Microarray Protocols for Tissue Glycome Mapping with
High-End Scanners.- Laser capture microdissection followed by DNA methylation
profiling.- High resolution laser microdissection to investigate
transcriptional states in formalin-fixed paraffin embedded samples.-
Transcriptomic analysis from cryosectioned tissue samples using laser capture
microdissection based Geo-seq.- Immunofluorescence imaging-guided laser
microdissection for ultra-low input spatial tissue proteomics.- Low-Input
Proteomics Protocols for Laser-Captured FFPE Samples with Orbitrap Astral
DIA-MS.- Laser capture microdissection followed by histone H1 variant
analysis by mass spectrometry.- A spatial proteomics approach to isolate and
analyze amyloid proteins using laser capture microdissection and mass
spectrometry.- Chromatin Immunoprecipitation-Sequencing and RNA-Sequencing
from Laser Microdissected Human Lung Cancer Cells.- Laser capture
microdissection followed by ATAC-seq.- Laser Capture Microdissection for
quantitative shotgun lipidomics.- Artificial Intelligence-driven laser
capture microdissection.- Automated Intelligent Microscopy for phenotypic
identification, spatial localization and retargeting of cells.