The emergence and re-emergence of new infectious diseases places emphasis on the need for greater investment in rapid healthcare diagnostic solutions. As a result, lateral flow immunoassay technology is quickly evolving with new inventions. Lateral Flow Assay for Rapid Point-of-Care Testing covers today’s most significant advancements, emerging trends, and future directions linked with this technology. The book begins with a review of the format, flow, and design of old-generation lateral flow assays and the challenges impeding performance before moving into innovation in materials for rapid test strips, advances in manipulating sample flow, and the latest developments in molecular imprinting and bioconjugation techniques. Subsequent sections cover various biorecognition elements and review signal amplification as well as quantification trends. Final chapters delve into recent applications for infectious diseases detection, monitoring antibiotic resistance, multiplex detection of analytes, as well as future directions leading to the next generation of healthcare. Academicians, researchers, industrialists, and device manufacturers linked to the development of rapid and affordable point-of-care immunodiagnostics will find value in this resource.
Section A. Lateral flow assay - format, flow, and design
1. Lateral flow assays and their role in healthcare
2. Innovations in materials used for the manufacturing of rapid test strip
3. Advances in manipulating sample flow in LFA
4. Molecular Imprinting and bioconjugation in lateral flow assays (LAFs)
Section B: Biorecognition elements in Lateral Flow Assays
5. Nucleic acids and Aptamers
6. CRISPR-cas based lateral flow assays for diagnosis
7. Molecularly imprinted polymers
Section C: Signal Amplification and Quantification
8. Fluorescent, upconverting, and SERS molecules
9. Lateral flow immunoassays (LFIA) with quantum dots as the reporter
10. Latest technology of signal acquisition-readers and devices
11. Integrated Data analysis with smartphones, internet, and AI
12. Visual semi-quantitation
Section D: Recent Applications
13. Advancements in LFA for infectious diseases detection
14. LFA technology for monitoring antibiotic resistance
15. Multiplex lateral flow assay
16. Emerging trends and future directions in LFA
Dr. Seema Nara is Professor at Department of Biotechnology, Motilal Nehru National Institute of Technology Allahabad, Prayagraj, India. She has been actively indulged in teaching and research activities at MNNIT Allahabad for the last fifteen years. Her research area mainly focuses on diagnostics for healthcare, applications of nanotechnology in diagnostics and therapeutics. She has published various research and review papers in peer reviewed journals of repute, reviews and book/book chapters in her areas of interests. She has three granted patents to her credit and has executed various extramural research projects.
Dr. Mithilesh Singh is Senior Scientist at Department of Immunology, ICAR-Indian Veterinary Research Institute, Bareilly, India. Dr Singh has been actively involved in research and teaching activities at IVRI, Bareilly for the last fourteen years. His research interest mainly focuses on animal health covering diagnostics, vaccines and therapeutics. He has published various scientific papers in peer reviewed journals and book chapters in his areas of interests. He has one filed patent to his credit and has been involved in various extramural research projects as project investigator and collaborators.
Dr. Sandeep K. Vashist is the Senior Director R&D at The Binding Site, Thermo Fisher Scientific, United Kingdom. He held senior R&D and management roles in large IVD giants, such as Bristol-Myers Squibb, Revvity, Trivitron, and Fapon. Besides, he has been the Chief of the Research Advisory Board at Sensing Self Pte Ltd., Singapore, and an Advisory Board Member at Calciscon AG, Switzerland; Fapon, China; and Antrum Biotech, South Africa. Sandeep invented and pioneered many CE-certified and/or FDA-approved IVD kits, devices, and technologies. He has 12 patents, over 300 publications, 5 books, and over 9900 international citations. He is the Editor and Reviewer of several journals, funding agencies, and international organizations. Sandeep is an IVD veteran with over two decades of very rich global IVD experience.
