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Macrophage Migration Inhibitory Factor: Methods and Protocols Second Edition 2026 [Kõva köide]

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  • Formaat: Hardback, 290 pages, kõrgus x laius: 254x178 mm, 83 Illustrations, color; 10 Illustrations, black and white
  • Sari: Methods in Molecular Biology
  • Ilmumisaeg: 24-Apr-2026
  • Kirjastus: Humana
  • ISBN-10: 1071650556
  • ISBN-13: 9781071650554
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Macrophage Migration Inhibitory Factor: Methods and Protocols Second Edition 2026Suurem pilt
  • Formaat: Hardback, 290 pages, kõrgus x laius: 254x178 mm, 83 Illustrations, color; 10 Illustrations, black and white
  • Sari: Methods in Molecular Biology
  • Ilmumisaeg: 24-Apr-2026
  • Kirjastus: Humana
  • ISBN-10: 1071650556
  • ISBN-13: 9781071650554
Teised raamatud teemal:
Püsilink: https://www.kriso.ee/db/9781071650554.html

This second edition volume expands on the previous edition with new protocols that offer biochemical, biophysical, and biological assays for studying macrophage migration inhibitory factor (MIF) and D-dopachrome tautomerase (D-DT). The chapters in this book cover topics such as generation of MIF peptides via solid phase peptide synthesis; fragment screening of MIF by surface plasmon resonance; MIF NMR chemical shift perturbation mapping; methods to determine the role of MIF in cilia biogenesis; and immunofluorescent quantification of MIF in larval zebrafish tailfin transfection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Macrophage Migration Inhibitory Factor: Methods and Protocols, Second Edition is a valuable resource for any researcher hoping to contribute to the ever expanding world of MIF biology.

Expression and Purification of Recombinant Macrophage Migration
Inhibitory Factor (MIF).- Generation of MIF Peptides via Solid Phase Peptide
Synthesis.- Expression and Purification of Recombinant D-Dopachrome
Tautomerase (D-DT).- Structural Determination and Ligand Interaction of
Macrophage Inhibitory Factor: A Standardized X-Ray Crystallography
Approach.- Generation of High-Quality D-Dopachrome Tautomerase Crystals for
Structural Studies.- Fragment Screening of MIF by Surface Plasmon Resonance.-
Detection of Noncovalent Protein-Ligand Complexes of D-DT Variants by
MALDI-TOF Mass Spectrometry.- Analysis of D-Dopachrome Tautomerase
Conformational Flexibility Through Molecular Dynamics Solutions.- MIF NMR
Chemical Shift Perturbation Mapping.- Assessing the Tautomerase Activity of
MIF Using L-Dopachrome.- Using 4-HPP and PP as Substrates to Assess Purified
MIF and D-DT Tautomerase Activity and Inhibition.- Protocols for Assessing
the Nuclease Activity of MIF.- Detection of Oxidized Macrophage Migration
Inhibitory Factor (oxMIF) in Biological Samples Using Enzyme-Linked
Immunosorbent Assay (ELISA).- Detection of Oxidized Macrophage Migration
Inhibitory Factor (oxMIF) in Tissue Samples Using Immunohistochemistry
(IHC).- Exploring the Chemotactic Effects of MIF on Neutrophils and T Cells
and Methods for Selective Receptor Targeting.- Polarization of Bone
Marrow-Derived Macrophages from Wild-Type and High Human MIF-Expressing
CATT7 Polymorphic Mice.- Using Luciferase Reporter Cells to Assess the
Effects of MIF Inhibitors on NF-kB Activation.- Monitoring Inflammasome
Activity through ASC Speck Formation in THP-1 ASC-GFP Cells.- Small
Interfering RNA (siRNA)-Mediated Knockdown of Macrophage Migration Inhibitory
Factor Transcript in Adherent and Suspension Cultures of Human Cell Lines.-
Stable Transfection of CD74 Gene Variants into A549 Lung Adenocarcinoma Cell
Line.- Methods to Determine the Role of MIF in Cilia Biogenesis.- Modelling
Fungal and Amoebic Infections: Co-Culture Approaches to Study MIFs Role in
Infection Induced Inflammatory Responses.- Immunofluorescent Quantification
of MIF in Larval Zebrafish Tailfin Transfection.