List of research focus boxes |
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xii | |
Preface |
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xiii | |
Acknowledgements |
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xv | |
Abbreviations |
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xvii | |
Glossary of key terms |
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xxi | |
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1 Microbes in the marine environment |
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1 | (18) |
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1.1 What is marine microbiology? |
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1 | (1) |
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1.2 Biological organization and the evolution of life |
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2 | (3) |
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2 | (1) |
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1.2.2 The nature of viruses |
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2 | (1) |
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1.2.3 Phylogenetic approaches to classifying the living world |
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3 | (1) |
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1.2.4 The three-domain tree of life |
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3 | (2) |
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1.3 The importance of microbes in the living world |
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5 | (1) |
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1.4 The importance of size |
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5 | (3) |
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1.5 The world's oceans and seas |
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8 | (1) |
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1.6 Chemical and physical factors in the marine environment |
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9 | (1) |
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1.6.1 Properties of seawater |
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9 | (1) |
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1.6.2 Solar radiation and temperature |
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10 | (1) |
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1.7 Marine microbial habitats |
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10 | (7) |
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1.7.1 The water column and marine snow |
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10 | (3) |
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13 | (1) |
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1.7.3 Microbial life at surfaces - biofilms and microbial mats |
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14 | (1) |
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14 | (1) |
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1.7.5 Hydrothermal vents and cold seeps |
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15 | (2) |
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1.7.6 Living organisms as microbial habitats |
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17 | (1) |
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References and further reading |
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17 | (2) |
2 Methods in marine microbiology |
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19 | (44) |
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2.1 Sampling and experimental approaches |
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19 | (2) |
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21 | (1) |
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21 | (1) |
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2.2.2 Electron microscopy |
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21 | (1) |
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2.2.3 Con focal laser scanning microscopy (CLSM) |
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21 | (1) |
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2.2.4 Epifluorescence light microscopy (ELM) |
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22 | (1) |
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22 | (2) |
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2.4 Antibody-labeling techniques |
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24 | (1) |
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25 | (5) |
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2.5.1 The importance of cultural conditions |
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25 | (1) |
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26 | (1) |
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26 | (2) |
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2.5.4 Biochemical methods for identification and taxonomy of bacteria |
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28 | (2) |
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30 | (13) |
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2.6.1 The impact of molecular tools in marine microbial diversity |
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30 | (2) |
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2.6.2 Isolation of nucleic acids |
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32 | (1) |
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2.6.3 The polymerase chain reaction (PCR) |
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33 | (3) |
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36 | (1) |
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2.6.5 Phylogenetic analysis |
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37 | (2) |
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2.6.6 Community fingerprinting |
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39 | (1) |
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2.6.7 Limitations of environmental analysis of nucleic acids |
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39 | (1) |
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2.6.8 Genomic fingerprinting and molecular markers |
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40 | (1) |
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41 | (1) |
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2.6.10 Fluorescent In situ hybridization (FISH) |
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42 | (1) |
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2.6.11 GC ratios and DNA-DNA hybridization in bacterial taxonomy |
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43 | (1) |
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2.7 Detecting microbial activities in the marine environment |
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43 | (3) |
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2.7.1 The microenvironment |
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43 | (1) |
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2.7.2 Microelectrodes and biosensors |
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44 | (1) |
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44 | (1) |
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2.7.4 Measurement of specific cell constituents |
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45 | (1) |
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45 | (1) |
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References and further reading |
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46 | (3) |
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3 Structure of marine prokaryotes |
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49 | (14) |
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3.1 Overview of the Bacteria and Archaea |
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49 | (1) |
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3.2 Cell morphology and structure |
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49 | (1) |
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3.3 Cytoplasmic and internal membranes |
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49 | (2) |
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51 | (1) |
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52 | (1) |
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3.6 Ribosomes and protein synthesis |
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53 | (1) |
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54 | (1) |
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3.8 Capsules and the glycocalyx |
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55 | (1) |
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3.9 Motility of marine bacteria |
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56 | (4) |
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56 | (1) |
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3.9.2 Chemotaxis and related behaviors |
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57 | (2) |
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59 | (1) |
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60 | (1) |
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References and further reading |
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60 | (3) |
4 Physiology of marine prokaryotes |
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63 | (22) |
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4.1 Metabolic diversity and the importance of microbial communities |
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63 | (1) |
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4.2 Modes of nutrition in marine prokaryotes |
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63 | (1) |
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4.3 Energy-yielding processes |
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63 | (4) |
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4.3.1 Methods of ATP generation |
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63 | (1) |
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4.3.2 Phototrophy and primary productivity |
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64 | (2) |
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66 | (1) |
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66 | (1) |
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66 | (1) |
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4.4 Nutrients needed for growth |
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67 | (4) |
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4.4.1 Macronutrients, micronutrients and trace elements |
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67 | (1) |
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67 | (1) |
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4.4.3 Carbon dioxide fixation in autotrophs |
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67 | (1) |
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4.4.4 Nitrification and denitrification |
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68 | (1) |
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69 | (1) |
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4.4.6 Sulfur and phosphorus |
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69 | (1) |
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70 | (1) |
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4.5 Growth and the effects of nutrient concentration |
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71 | (6) |
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4.5.1 The bacterial growth cycle |
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71 | (1) |
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72 | (1) |
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4.5.3 Effects of nutrient concentration |
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73 | (2) |
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4.5.4 Growth on surfaces - microbial interactions and biofilm communities |
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75 | (2) |
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4.6 Extreme environmental conditions |
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77 | (5) |
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77 | (1) |
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77 | (1) |
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78 | (2) |
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4.6.4 Toxic effects of oxygen |
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80 | (1) |
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4.6.5 Ultraviolet (UV) irradiation |
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81 | (1) |
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4.6.6 High salt concentrations |
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81 | (1) |
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References and further reading |
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82 | (3) |
5 Marine Bacteria |
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85 | (30) |
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5.1 Approaches to the study of prokaryotic diversity |
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85 | (1) |
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5.2 Prokaryote diversity In marine ecosystems revealed by culture-independent methods |
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86 | (2) |
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5.2.1 Cloning of 16S rRNA sequences from the environment |
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86 | (1) |
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5.2.2 The major phylogenetic groups of planktonic Bacteria |
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87 | (1) |
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5.3 Anoxygenic phototrophic bacteria |
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88 | (3) |
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5.3.1 Purple sulfur and nonsulfur bacteria |
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88 | (2) |
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5.3.2 Roseobacter and Erythrobacter |
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90 | (1) |
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5.3.3 Green sulfur bacteria |
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91 | (1) |
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5.4 Oxygenic phototrophs - the Cyanobacteria |
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91 | (3) |
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5.4.1 Nature of the Cyanobacteria |
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91 | (1) |
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5.4.2 Morphology and taxonomy |
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92 | (1) |
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92 | (1) |
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5.4.4 Prochlorococcus and Synechococcus |
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93 | (1) |
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5.4.5 Microbial mats and stromatolites |
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94 | (1) |
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5.5 The nitrifying bacteria |
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94 | (1) |
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5.6 Sulfur- and iron-oxidizing chemolithotrophs |
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95 | (2) |
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5.6.1 Thiobacillus, Beggiatoa, Thiothrix and Thiovulum |
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95 | (1) |
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5.6.2 Thioploca and Thiomargarita |
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96 | (1) |
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5.7 Hydrogen-oxidizing bacteria |
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97 | (1) |
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5.8 Aerobic methanotrophs and methylotrophs |
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97 | (1) |
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5.9 Pseudomonas, Alteromonas and Shewanella |
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98 | (1) |
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5.10 Free-living aerobic nitrogen-fixing bacteria |
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98 | (1) |
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5.11 The Enterobacteriaceae |
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99 | (1) |
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5.12 Vibrio and related genera |
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99 | (1) |
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5.12.1 Vibrio, Photobacterium, Aeromonas and related genera |
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99 | (1) |
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100 | (1) |
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5.12.3 Regulation of bioluminescence |
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102 | (2) |
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104 | (1) |
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105 | (1) |
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5.14.1 Oceanospirillum and related genera |
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105 | (1) |
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5.14.2 Magnetotactic bacteria |
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106 | (1) |
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106 | (1) |
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5.15 Budding and stalked Proteobacteria |
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107 | (1) |
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5.16 Planctomycetes - stalked bacteria |
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107 | (1) |
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5.17 Sulfur- and sulfate-reducing Proteobacteria |
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108 | (1) |
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5.18 Gram-positive Bacteria |
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109 | (1) |
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5.18.1 Endospore- formers - Bacillus and Clostridium |
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109 | (1) |
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110 | (1) |
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5.18.3 Epuloposcium fishelsoni |
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110 | (1) |
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5.18.4 Actinobacteria - mycobacteria and actinomycetes |
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111 | (1) |
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5.19 The Cytophaga-Flavobacterium-Bacteroides (CFB) group |
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111 | (1) |
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111 | (1) |
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112 | (1) |
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5.22 'Deeply branching' hyperthermophiles |
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112 | (1) |
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112 | (1) |
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112 | (1) |
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References and further reading |
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112 | (3) |
6 Marine Archaea |
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115 | (10) |
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6.1 Phylogenetic groups in the domain Archaea |
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115 | (3) |
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118 | (3) |
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118 | (1) |
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6.2.2 Hyperthermophilic chemoorganotrophs - Thermococcus and Pyrococcus |
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119 | (1) |
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6.2.3 Hyperthermophilic sulfate-reducers and iron-oxidizers - Archaeoglobus and Ferroglobus |
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120 | (1) |
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120 | (1) |
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121 | (2) |
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6.3.1 The diversity of Crenarchaeota |
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121 | (1) |
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6.3.2 Hyperthermophiles - the Desulfurococcales |
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121 | (1) |
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6.3.3 The uncultured psychrophilic marine Crenarchaeota |
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122 | (1) |
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References and further reading |
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123 | (2) |
7 Marine eukaryotic microbes |
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125 | (12) |
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7.1 Introduction to the protists and fungi |
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125 | (1) |
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7.2 Overview of eukaryotic cell structure and function |
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125 | (2) |
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7.3 Nanoplanktonic flagellates |
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127 | (1) |
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128 | (2) |
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7.4.1 Bioluminescence and biological clocks |
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130 | (1) |
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130 | (1) |
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131 | (2) |
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133 | (1) |
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7.8 Radiolarians and foraminifera |
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134 | (1) |
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135 | (1) |
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References and further reading |
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136 | (1) |
8 Marine viruses |
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137 | (10) |
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8.1 The nature of marine viruses |
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137 | (1) |
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8.2 Viruses infecting prokaryotes |
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138 | (1) |
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8.3 Enumerating viruses and virus-like particles |
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139 | (1) |
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8.4 Morphology of marine viruses |
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140 | (1) |
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8.5 Estimates of virus abundance |
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140 | (1) |
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8.6 Observing phage-infected cells |
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141 | (1) |
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141 | (1) |
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142 | (1) |
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142 | (1) |
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8.10 Effect of viruses on plankton mortality |
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143 | (2) |
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8.11 Viruses of eukaryotic plankton |
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145 | (1) |
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References and further reading |
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145 | (2) |
9 The role of microbes in ocean processes |
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147 | (20) |
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147 | (1) |
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9.2 Carbon cycling in the oceans |
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148 | (2) |
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9.3 Photosynthesis and primary productivity |
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150 | (1) |
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9.4 Productivity and nutrients |
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151 | (8) |
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9.4.1 Nutrient limitation |
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151 | (2) |
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9.4.2 Microbial aspects of nitrogen cycling |
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153 | (1) |
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9.4.3 The importance of iron |
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154 | (5) |
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9.5 The microbial loop in ocean food webs |
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159 | (4) |
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9.5.1 Classic and modern food webs compared |
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159 | (1) |
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9.5.2 The formation and fate of DOM and POM |
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160 | (1) |
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161 | (1) |
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162 | (1) |
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9.6 Microbial processes in eutrophication of coastal waters |
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163 | (1) |
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9.7 Microbial processes and climate |
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163 | (1) |
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References and further reading |
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164 | (3) |
10 Symbiotic associations |
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167 | (16) |
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167 | (1) |
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10.2 Symbioses of microalgae with animals |
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167 | (1) |
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10.2.1 Types of association |
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167 | (1) |
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10.2.2 Nature of dinoflagellate endosymbionts (zooxanthellae) |
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167 | (1) |
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170 | (1) |
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171 | (1) |
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10.3 Symbioses of chemoautotrophic prokaryotes with animals |
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171 | (1) |
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10.3.1 Chemoautotrophic endosymbionts in hydrothermal vent animals |
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171 | (1) |
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10.3.2 Episymbiotic bacteria on vent animals |
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173 | (1) |
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10.3.3 Chemoautotrophic endosymbionts in non-vent animals |
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173 | (1) |
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10.3.4 Phylogeny and acquisition of symbiotic bacteria |
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173 | (2) |
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10.4 Light organ symbioses in fish and invertebrates |
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175 | (1) |
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10.4.1 Flashlight fishes and anglerfishes |
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175 | (1) |
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10.4.2 Sepiolids (bobtail squids) |
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176 | (1) |
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10.5 Microbial symbionts of sponges |
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177 | (1) |
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10.6 Symbiosis and mixotrophy in protists |
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177 | (3) |
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10.7 Metabolic consortia and mutualism between prokaryotes |
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180 | (1) |
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References and further reading |
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180 | (3) |
11 Human disease - bacteria and viruses |
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183 | (20) |
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11.1 Mechanisms of pathogenicity |
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183 | (1) |
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11.2 Indigenous marine bacteria |
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184 | (1) |
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184 | (1) |
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189 | (1) |
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11.2.3 Vibrio parahaemolyticus |
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189 | (1) |
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11.2.4 Clostridium botulinum |
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190 | (1) |
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11.2.5 Scombroid fish poisoning |
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190 | (1) |
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11.2.6 Pufferfish (Fugu) poisoning |
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190 | (1) |
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11.3 Health hazards from sewage pollution at sea |
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191 | (1) |
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11.3.1 Sewage as a source of bacterial and viral infections |
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191 | (1) |
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11.3.2 Monitoring for potential pathogens - the indicator concept |
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192 | (1) |
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11.3.3 Coli forms and Escherichia coli |
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193 | (1) |
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11.3.4 Fecal streptococci (enterococci) |
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193 | (1) |
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11.3.5 Quality standards for recreational marine waters |
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195 | (1) |
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11.3.6 Shell fish hygiene |
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196 | (1) |
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11.3.7 Alternative indicators |
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198 | (1) |
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11.3.8 Direct testing for pathogens |
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199 | (1) |
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11.4 Heavy metal mobilization |
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199 | (1) |
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References and further reading |
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200 | (3) |
12 Human disease - toxic dinoflagellates and diatoms |
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203 | (14) |
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12.1 'Red tides' and 'harmful algal blooms' |
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203 | (1) |
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203 | (1) |
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12.2.1 Paralytic shell fish poisoning (PSP) |
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205 | (1) |
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12.2.2 Neurotoxic shell fish poisoning (NSP) |
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205 | (1) |
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12.2.3 Diarrhetic shell fish poisoning (DSP) and azaspiracid poisoning |
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206 | (1) |
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12.2.4 Amnesic shell fish poisoning (ASP) |
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207 | (1) |
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12.3 Ciguatera fish poisoning (CFP) |
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208 | (1) |
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12.4 Pfiesteria piscicida |
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209 | (2) |
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12.5 Why do dinoflagellates and diatoms produce toxins? |
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211 | (1) |
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12.6 Why are HABs and toxin-associated diseases increasing? |
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211 | (2) |
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12.7 Monitoring and control of HABs |
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213 | (1) |
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References and further reading |
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214 | (3) |
13 Diseases of marine mammals |
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217 | (6) |
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13.1 Difficulties of study |
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217 | (1) |
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13.2 Effects of microalgal toxins |
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217 | (1) |
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218 | (1) |
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218 | (1) |
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220 | (1) |
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13.4 Bacterial and fungal infections |
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220 | (1) |
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13.5 Effects of environmental pollution on infectious diseases |
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221 | (1) |
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221 | (1) |
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References and further reading |
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222 | (1) |
14 Microbial diseases of fish |
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223 | (20) |
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14.1 Importance in wild fish and in aquaculture |
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223 | (1) |
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223 | (1) |
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14.3 Bacterial infections |
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224 | (1) |
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14.3.1 Mechanisms of pathogenicity |
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224 | (1) |
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224 | (1) |
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14.3.3 Photobacterium damselae subsp. piscicida |
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227 | (1) |
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14.3.4 Aeromonas salmonicida |
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228 | (1) |
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14.3.5 Piscirickettsia salmonis |
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229 | (1) |
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14.3.6 Renibacterium salmoninarum |
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229 | (1) |
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14.3.7 Tenacibacter maritimus |
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231 | (1) |
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14.3.8 Mycobacterium and Nocardia |
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231 | (1) |
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14.3.9 Lactococcus and Streptococcus |
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231 | (1) |
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232 | (1) |
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232 | (1) |
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14.4.2 Infectious pancreatic necrosis virus (IPNV) |
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232 | (1) |
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14.4.3 Infectious salmon anemia virus (ISAV) |
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232 | (1) |
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14.4.4 Other virus infections |
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232 | (1) |
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14.5 Control of infectious disease of fish |
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233 | (1) |
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14.5.1 Hrtsbandry and health management in mariculture |
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233 | (1) |
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14.5.2 Treatment-antimicrobial agents |
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233 | (1) |
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14.5.3 Vaccines, immunostimulants and probiotics |
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236 | (4) |
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14.6 Protistan infections and HABs |
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240 | (1) |
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References and further reading |
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240 | (3) |
15 Diseases of invertebrates |
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243 | (12) |
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243 | (1) |
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15.2 Bacterial and viral diseases of bivalve molluscs |
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243 | (1) |
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243 | (1) |
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243 | (2) |
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15.3 Bacterial and viral diseases of crustaceans |
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245 | (1) |
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15.3.1 Diseases in aquaculture |
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245 | (1) |
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246 | (1) |
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15.3.3 Rickettsias and mycoplasmas |
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246 | (1) |
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15.3.4 Aerococcus viridans var. homari |
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246 | (1) |
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247 | (1) |
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15.3.6 Control of disease in crustaceans |
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247 | (1) |
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248 | (4) |
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References and further reading |
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252 | (3) |
16 Marine microbes and human society |
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255 | (18) |
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16.1 Beneficial and detrimental effects |
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255 | (1) |
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16.2 Biofouling and biodeterioration |
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255 | (1) |
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16.2.1 Biofilms and biofouling |
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255 | (1) |
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16.2.2 Biodeterioration of metals and wood |
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259 | (1) |
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16.3 Biodegradation and bioremediation of marine pollutants |
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260 | (1) |
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|
260 | (1) |
|
16.3.1.1 Sources of oil in the sea |
|
|
260 | (1) |
|
|
260 | (1) |
|
|
261 | (2) |
|
16.3.2 Persistent organic pollutants and plastics |
|
|
263 | (1) |
|
|
263 | (1) |
|
16.4 Environmental monitoring |
|
|
264 | (1) |
|
16.5 Microbiology of fish and seafood products |
|
|
264 | (2) |
|
|
266 | (2) |
|
|
268 | (1) |
|
16.8 Biomedical and health products |
|
|
268 | (1) |
|
16.9 Biomimetics, nanotechnology and bioelectronics |
|
|
269 | (2) |
|
References and further reading |
|
|
271 | (2) |
17 Concluding remarks |
|
273 | |
|
References and further reading |
|
|
274 | |