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Methods in Cardiovascular and Lymphatic Biology [Kõva köide]

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  • Formaat: Hardback, 188 pages, kõrgus x laius: 254x178 mm, 48 Illustrations, color; 6 Illustrations, black and white; IV, 188 p. 54 illus., 48 illus. in color., 1 Hardback
  • Sari: Methods in Molecular Biology 2956
  • Ilmumisaeg: 13-Sep-2025
  • Kirjastus: Springer-Verlag New York Inc.
  • ISBN-10: 1071647059
  • ISBN-13: 9781071647059
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Methods in Cardiovascular and Lymphatic BiologySuurem pilt
  • Formaat: Hardback, 188 pages, kõrgus x laius: 254x178 mm, 48 Illustrations, color; 6 Illustrations, black and white; IV, 188 p. 54 illus., 48 illus. in color., 1 Hardback
  • Sari: Methods in Molecular Biology 2956
  • Ilmumisaeg: 13-Sep-2025
  • Kirjastus: Springer-Verlag New York Inc.
  • ISBN-10: 1071647059
  • ISBN-13: 9781071647059
Teised raamatud teemal:
Püsilink: https://www.kriso.ee/db/9781071647059.html

This volume provides an overview of advanced protocols for vascular biology research, comprising cardiovascular and lymphatic biology. Chapters detail techniques for isolating and characterizing endothelial and mural cell populations, disseminating signaling pathways and protein-protein interactions, constructing organ-on-chip systems, and using animal models and techniques with bench-to-bedside potential. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and up-to-date, Methods in Cardiovascular and Lymphatic Biology aims to provide protocols and applications that can be used by both new investigators and experienced professionals to advance cardiovascular and lymphatic research.

Isolation and purification of murine lung endothelial cells.- Two-step
Positive Selection Method for the Magnetic Isolation of Lymphatic Endothelial
Cells.- Isolation and Enumeration of Circulating Endothelial Cells with
Antibody Labeled Staggered Herringbone Microfluidic System.- A Guided
Approach to Establish a Functional Humanized Brain-on-Chip Microfluidic Model
of the Neurovascular System.- Human iPSC-derived astrocyte-endothelial cell
and neuron-endothelial cell co-culture models of the blood-brain barrier to
study the impact of ischemic stroke in vitro.- Isolation of rodent aortic
smooth muscle cells by enzymatic digestions.- Studying the vascular tone of
mouse aortic rings in a standard wire myograph.- Unveiling Central Nervous
System Pericytes: Immunofluorescence Protocol and Confocal Microscopy.-
Serum-free Mouse Aortic Ring Assay of Angiogenesis.- A New Method to
Visualize Retinal Deep Vascular Layers Using Wholemount Tissue Preparations.-
Live calcium imaging on mouse primary brain endothelial cells.- Studying
nitric oxide in endothelial cells.- Identifying protein-protein interaction
or protein phosphorylation by Proximity Ligation Assay (PLA) in endothelial
cells.- Label-free imaging with automated tracking and cell-by-cell analysis
of migration, morphology, and differentiation of endothelial cells.-
Assessment of angiogenic properties of malignant pleural effusion (MPE)
fluid.- Ultrasound Contrast Agents in Mice: Bridging the Gap from Preclinical
Research to Clinical Translation.