| Laboratory Safety Rules |
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vii | |
| Laboratory Safety Rules Contract |
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xiii | |
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Lab 1 Ubiquity of Microorganisms (2-Day Lab) |
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1 | (8) |
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Table 1-1 Colony Characteristics |
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3 | (2) |
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Diagram 1 Ubiquity of Microorganisms Plate |
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5 | (1) |
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6 | (1) |
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7 | (2) |
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Lab 2 Microscopy (3-Day Lab) |
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9 | (20) |
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Diagram 2 Giardia intestinalis (400× prepared slide) |
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16 | (1) |
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Diagram 3 Cryptosporidium parvum (400× prepared slide) |
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17 | (1) |
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Diagram 4 Trypanosoma brucei (400× prepared slide) |
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18 | (1) |
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Diagram 5 Bacteria---Three Types (1000× prepared slide) |
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19 | (1) |
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Diagram 6 Treponema pallidum (1000× prepared slide) |
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20 | (1) |
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Diagram 7 Bacillus subtilis (1000× prepared slide) |
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21 | (1) |
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Diagram 8 Proteus vulgaris (1000× prepared slide) |
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22 | (1) |
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Diagram 9 Klebsiella pneumonia (1000× prepared slide) |
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23 | (1) |
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Diagram 10 Escherichia coli (1000× stained with methylene blue) |
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24 | (1) |
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Diagram 11 Unknown Bacterium from Lab 1: Ubiquity of Microorganisms (1000× stained with methylene blue) |
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25 | (1) |
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26 | (3) |
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Lab 3 Gram Stain (2-Day Lab) |
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29 | (8) |
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Diagram 12 Unknown Bacterium from Lab 1: Ubiquity of Microorganisms (1000× stained with Gram stain) |
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34 | (1) |
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35 | (2) |
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Lab 4 Bacterial Growth Media |
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37 | (6) |
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41 | (2) |
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Lab 5 Serial Dilutions (2-Day Lab) |
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43 | (10) |
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Diagram 13 Serial Dilution Schematic |
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47 | (1) |
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Table 5-1 Serial Dilution Plate Count |
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47 | (1) |
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Diagram 14 Example of a Statistically Valid Plate |
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48 | (1) |
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49 | (1) |
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50 | (3) |
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Lab 6 Viral Plaques (2-Day Lab) |
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53 | (8) |
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Diagram 15 Serial Dilution Schematic |
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56 | (1) |
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Table 6-1 Serial Dilution Plate Count |
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56 | (1) |
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Diagram 16 Example of a Statistically Valid Plate |
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57 | (1) |
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57 | (1) |
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58 | (3) |
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Lab 7 Growth Curve and Viable Plate Count (2-Day Lab) |
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61 | (10) |
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Table 7-1 Reporting Growth Curve Data |
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65 | (1) |
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Bacterial Growth Curve (Time vs. CFU/mL, semi-log graph) |
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66 | (1) |
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Bacterial Growth Curve (Time vs A660, linear graph) |
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67 | (1) |
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Comparison of CFU/ml to A660 |
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68 | (1) |
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69 | (1) |
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70 | (1) |
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71 | (8) |
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74 | (1) |
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74 | (1) |
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Figure 8-1 Mean Percent Kill as a Function of UVGI Dose |
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75 | (1) |
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76 | (1) |
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77 | (2) |
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Lab 9 Differentiation of Gram Positive Cocci (3-Day Lab) |
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79 | (12) |
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Table 9-1 Metabolic and Anatomic Differences between Cocci |
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83 | (1) |
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84 | (1) |
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85 | (1) |
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Diagram 19 TSA with 5% Sheep's Blood Plate |
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86 | (1) |
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Diagram 20 Throat Swab on TSA with 5% Sheep's Blood |
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87 | (1) |
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Diagram 21 Body Swab on MSA |
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88 | (1) |
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89 | (1) |
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90 | (1) |
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Lab 10 Identification of MRSA Strains (2-Day Lab) |
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91 | (8) |
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Table 10-1 Interpretation of Zone of Inhibition of S. aureus Strains when Challenged with Selected Antibiotics |
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93 | (1) |
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Table 10-2 Interpretation of Zone of Inhibition of S. aureus Strains when Challenged with Selected Antibiotics |
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93 | (1) |
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Diagram 22 Kirby Bauer Disk Diffusion Plates |
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94 | (1) |
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95 | (1) |
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96 | (3) |
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Lab 11 Identification of Enterics (2--4 Day Lab) |
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99 | (12) |
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Table 11-1 Metabolic Results on Selected Enterics |
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103 | (1) |
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Diagram 23 Metabolic Test on Stock Enterics |
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104 | (1) |
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Diagram 24 EMB Streak Plate of Isolated/Unknown Enteric |
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105 | (1) |
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Diagram 25 Gram Stain of Isolated/Unknown Enteric |
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106 | (1) |
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Diagram 26 Metabolic Test on Isolated/Unknown Enteric |
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107 | (1) |
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108 | (1) |
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109 | (2) |
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111 | (6) |
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114 | (1) |
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115 | (1) |
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116 | (1) |
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Lab 13 Bacterial Conjugation |
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117 | (6) |
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120 | (1) |
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121 | (1) |
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122 | (1) |
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123 | (16) |
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Table A-1 Summary of Bacterial Strains |
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123 | (1) |
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Table A-2 Metabolic and Anatomic Differences between Cocci (Key) |
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124 | (1) |
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Table A-3 Metabolic Results on Selected Enterics Key |
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124 | (1) |
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Diagram A-1 Bacillus subtilis (1000× stained with Gram stain) |
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125 | (1) |
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Diagram A-2 Citrobacter freundii (1000× stained with Gram stain) |
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126 | (1) |
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Diagram A-3 Enterobacter aerogenes (1000× stained with Gram stain) |
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127 | (1) |
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Diagram A-4 Escherichia coli (1000× stained with Gram stain) |
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128 | (1) |
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Diagram A-5 Klebsiella pneumonia (1000× stained with Gram stain) |
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129 | (1) |
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Diagram A-6 Proteus vulgaris (1000× stained with Gram stain) |
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130 | (1) |
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Diagram A-7 Salmonella typhimurium (1000× stained with Gram stain) |
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131 | (1) |
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Diagram A-8 Sarcina lutea (1000× stained with Gram stain) |
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132 | (1) |
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Diagram A-9 Serratia marcescens (1000× stained with Gram stain) |
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133 | (1) |
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Diagram A-10 Staphylococcus aureus (1000× stained with Gram stain) |
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134 | (1) |
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Diagram A-11 Staphylococcus epidermidis (1000× stained with Gram stain) |
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135 | (1) |
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Diagram A-12 Streptococcus pyogenes (1000× stained with Gram stain) |
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136 | (1) |
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137 | (1) |
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138 | (1) |
| Diagram A-15 |
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139 | (1) |
| Additional Notes |
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140 | |
