Contributors |
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xi | |
Editor Biographies |
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xv | |
Preface to the first edition |
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xvii | |
Preface to the second edition |
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xxi | |
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1 Materials and methods for microfabrication of microfluidic devices |
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1 | (78) |
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1 | (1) |
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1.2 Microfabrication methods |
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2 | (6) |
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8 | (38) |
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1.4 Conclusion and future trends |
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46 | (2) |
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48 | (31) |
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49 | (30) |
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2 Surface coatings for microfluidic biomedical devices |
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79 | (46) |
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79 | (2) |
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2.2 Covalent immobilization strategies: polymer devices |
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81 | (11) |
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2.3 Covalent immobilization strategies: glass devices |
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92 | (4) |
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2.4 Adsorption strategies |
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96 | (6) |
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2.5 Other strategies utilizing surface treatments |
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102 | (2) |
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2.6 Examples of applications |
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104 | (4) |
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2.7 Conclusions and future trends |
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108 | (2) |
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2.8 Sources of further information and advice |
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110 | (15) |
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111 | (14) |
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3 Actuation mechanisms for microfluidic biomedical devices |
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125 | (38) |
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125 | (1) |
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126 | (17) |
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143 | (9) |
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3.4 Limitations and future trends |
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152 | (11) |
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153 | (10) |
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4 Droplet microfluidics for biomedical devices |
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163 | (42) |
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4.1 Introduction---droplets in the wider context of microfluidics |
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163 | (2) |
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4.2 Fundamental principles of droplet microfluidics |
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165 | (6) |
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4.3 Droplet microfluidic approaches |
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171 | (4) |
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4.4 Biomedical applications |
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175 | (13) |
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4.5 Conclusion---perspective on the future of biomedical applications using droplet microfluidics |
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188 | (17) |
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190 | (15) |
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5 Controlled drug delivery using microdevices |
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205 | (20) |
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205 | (2) |
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5.2 Microreservoir-based drug delivery systems |
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207 | (5) |
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5.3 Micro/nanofluidics-based drug delivery systems |
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212 | (8) |
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5.4 Future trends and challenges |
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220 | (5) |
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221 | (4) |
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6 Microneedles for drug delivery and monitoring |
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225 | (36) |
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225 | (1) |
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6.2 Microneedle design parameters and structure |
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226 | (4) |
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6.3 Drug delivery strategies using microneedle arrays |
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230 | (7) |
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6.4 Other microneedle array applications |
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237 | (2) |
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6.5 Microneedle-mediated patient monitoring and diagnosis |
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239 | (8) |
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6.6 Clinical translation and commercialisation of microneedle products |
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247 | (3) |
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250 | (11) |
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250 | (11) |
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7 Microfluidic systems for drug discovery, pharmaceutical analysis, and diagnostic applications |
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261 | (68) |
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261 | (2) |
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7.2 Microfluidics for drug discovery |
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263 | (26) |
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7.3 Microfluidics for pharmaceutical analysis and diagnostic applications |
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289 | (21) |
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7.4 Examples of commercial microfluidic devices |
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310 | (1) |
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311 | (18) |
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312 | (17) |
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8 Microfluidic devices for cell manipulation |
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329 | (62) |
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329 | (1) |
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8.2 Microenvironment on cell integrity |
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330 | (2) |
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8.3 Microscale fluid dynamics |
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332 | (6) |
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8.4 Manipulation technologies |
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338 | (30) |
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8.5 Manipulation of cancer cells in microfluidic systems |
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368 | (6) |
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8.6 Conclusion and future trends |
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374 | (1) |
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8.7 Sources of further information and advice |
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374 | (17) |
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375 | (16) |
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9 Microfluidic devices for immobilization and micromanipulation of single cells and small organisms |
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391 | (22) |
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391 | (2) |
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9.2 Glass microfluidic device for rapid single cell immobilization and microinjection |
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393 | (4) |
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9.3 Microfluidic device for automated, high-speed microinjection of C. elegans |
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397 | (4) |
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9.4 Microfabricated device for immobilization and mechanical stimulation of Drosophila larvae |
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401 | (5) |
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9.5 Conclusions and outlook |
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406 | (7) |
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407 | (6) |
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10 Microfluidic devices for developing tissue scaffolds |
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413 | (24) |
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413 | (1) |
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10.2 Key issues and technical challenges for successful tissue engineering |
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414 | (5) |
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10.3 Microfluidic device platforms |
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419 | (9) |
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10.4 Conclusion and future trends |
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428 | (9) |
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429 | (8) |
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11 Microfluidic devices for stem cell analysis |
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437 | (52) |
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437 | (3) |
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11.2 Technologies used in stem cell analysis |
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440 | (10) |
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11.3 Examples of microfluidic platform for stem cell analysis: stem cell culture platform---mimicking in vivo culture conditions in vitro |
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450 | (8) |
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11.4 Examples of microfluidic platform for stem cell analysis: single stem cell analysis |
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458 | (3) |
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11.5 Microdevices for label-free and noninvasive monitoring of stem cell differentiation |
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461 | (6) |
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11.6 Microfluidics stem cell separation technology |
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467 | (8) |
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11.7 Conclusion and future trends |
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475 | (3) |
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11.8 Sources of further information and advice |
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478 | (11) |
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478 | (11) |
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12 Development of the immunoassay of antibodies and cytokines on nanobioarray chips |
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489 | (22) |
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12.1 Introduction to immunoassays |
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489 | (2) |
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491 | (3) |
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12.3 Immobilization chemistry |
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494 | (3) |
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497 | (2) |
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499 | (8) |
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12.6 Conclusion and future trends |
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507 | (4) |
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507 | (4) |
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13 Integrated microfluidic systems for genetic analysis |
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511 | (40) |
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511 | (2) |
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13.2 Integrated microfluidic systems |
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513 | (1) |
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13.3 Development of integrated microdevices |
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513 | (5) |
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13.4 Applications of fully integrated systems in genetic analysis |
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518 | (17) |
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13.5 Future of integrated microfluidic systems |
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535 | (16) |
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536 | (15) |
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14 Paper-based microfluidic devices for low-cost assays |
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551 | (36) |
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551 | (1) |
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14.2 Fabrication techniques for paper-based microfluidic devices |
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552 | (11) |
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14.3 Detection and read-out technologies |
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563 | (10) |
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14.4 Application of paper-based microfluidic devices |
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573 | (6) |
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14.5 Current limitations and future perspectives in paper-based microfluidics |
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579 | (8) |
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581 | (6) |
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15 Microfluidic devices for viral detection |
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587 | (30) |
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587 | (1) |
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15.2 Microfluidic technologies used for viral detection |
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588 | (15) |
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15.3 Examples of applications |
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603 | (6) |
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15.4 Conclusion and future trends |
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609 | (8) |
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610 | (1) |
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610 | (7) |
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16 Microfluidic applications on pancreatic islets and B-cells study for human islet transplant |
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617 | (42) |
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617 | (6) |
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16.2 Microfluidic technologies: the emergence of microfluidics applied to islet transplantation |
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623 | (9) |
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16.3 Design and validation of microfluidic devices for islet study and transplantation |
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632 | (7) |
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639 | (4) |
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16.5 Protocol: procedures |
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643 | (8) |
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16.6 Conclusion and future trends |
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651 | (8) |
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652 | (1) |
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652 | (7) |
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17 3D printed microfluidic devices and applications |
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659 | (22) |
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659 | (4) |
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17.2 Direct 3D printing of microfluidic devices and applications |
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663 | (8) |
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17.3 3D-printing of molds for fabricating PDMS microfluidic devices and applications |
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671 | (6) |
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17.4 Conclusions and future trends |
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677 | (4) |
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678 | (3) |
Index |
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