| Contributors |
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| Preface |
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Section I NOVEL AND EMERGING TECHNOLOGIES |
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1 Nucleic Acid Amplification Methods Overview |
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3 | (16) |
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2 Application of Identification of Bacteria by DNA Target Sequencing in a Clinical Microbiology Laboratory |
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19 | (13) |
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3 Microbial Whole-Genome Sequencing: Applications in Clinical Microbiology and Public Health |
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32 | (17) |
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4 Digital PCR and Its Potential Application to Microbiology |
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49 | (9) |
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5 Massively Parallel DNA Sequencing and Microbiology |
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58 | (10) |
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6 Next-Generation Sequencing |
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68 | (12) |
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80 | (12) |
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8 Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Microbial Identification in Clinical Microbiology |
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92 | (10) |
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102 | (15) |
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Section II METAGENOMICS: IMPLICATIONS FOR DIAGNOSTICS |
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10 The Skin Microbiome: Insights into Potential Impact on Diagnostic Practice |
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117 | (9) |
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11 The Gastrointestinal Microbiome |
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126 | (12) |
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12 The Vaginal Microbiome |
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138 | (8) |
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13 Microbial Communities of the Male Urethra |
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146 | (10) |
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14 The Human Virome in Health and Disease |
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156 | (13) |
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Section III HEALTH CARE-ASSOCIATED INFECTIONS |
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15 Molecular Detection of Staphylococcus aureus Colonization and Infection |
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169 | (9) |
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16 Molecular Diagnostics for Clostridium difficile |
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178 | (19) |
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17 Overview of Molecular Diagnostics in Multiple-Drug-Resistant Organism Prevention: Focus on Multiple-Drug-Resistant Gram-Negative Bacterial Organisms |
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197 | (15) |
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18 Detection of Vancomycin-Resistant Enterococci |
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212 | (23) |
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Section IV MOLECULAR DIAGNOSTICS AND PUBLIC HEALTH |
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19 The Impact of Molecular Diagnostics on Surveillance of Foodborne Infections |
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235 | (10) |
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20 Role of Molecular Methods in Improving Public Health Surveillance of Infections Caused by Antimicrobial-Resistant Bacteria in Health Care and Community Settings |
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245 | (11) |
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21 Molecular Diagnostics: Huge Impact on the Improvement of Public Health in China |
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256 | (10) |
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22 Surveillance and Epidemiology of Norovirus Infections |
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266 | (9) |
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23 Molecular Diagnostic Assays for the Detection and Control of Zoonotic Diseases |
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275 | (12) |
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Section V SYNDROMIC DIAGNOSTICS |
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24 Molecular Approaches to the Diagnosis of Meningitis and Encephalitis |
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287 | (19) |
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25 Using Nucleic Acid Amplification Techniques in a Syndrome-Oriented Approach: Detection of Respiratory Agents |
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306 | (30) |
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26 Molecular and Mass Spectrometry Detection and Identification of Causative Agents of Bloodstream Infections |
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336 | (26) |
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27 Molecular Diagnosis of Gastrointestinal Infections |
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362 | (24) |
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28 Diagnostic Approaches to Genitourinary Tract Infections |
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386 | (15) |
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29 Syndromic Diagnostic Approaches to Bone and Joint Infections |
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401 | (29) |
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30 Molecular Detection and Characterization of Human Immunodeficiency Virus Type 1/417 |
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31 Molecular Detection and Characterization of Hepatitis C Virus |
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430 | (19) |
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32 Molecular Detection and Characterization of Hepatitis B Virus |
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449 | (16) |
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33 Molecular Detection of Human Papillomaviruses |
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465 | (11) |
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34 Molecular Diagnostics for Viral Infections in Transplant Recipients |
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476 | (13) |
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Section VII FUNGI AND PROTOZOA |
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35 Molecular Detection and Identification of Fungal Pathogens |
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489 | (12) |
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36 Molecular Approaches for Diagnosis of Chagas' Disease and Genotyping of Trypanosoma cruzi |
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501 | (15) |
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37 Molecular Approaches for Diagnosis of Malaria and the Characterization of Genetic Markers for Drug Resistance |
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516 | (14) |
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Lisa C. Ranford-Cartwright |
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38 Molecular Detection of Gastrointestinal Parasites |
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530 | (15) |
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Section VIII POINT-OF-CARE/NEAR-CARE DIAGNOSTICS |
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39 Molecular Diagnostics and the Changing Face of Point-of-Care |
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545 | (11) |
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40 Point-of-Care Technologies for the Diagnosis of Active Tuberculosis |
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556 | (24) |
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41 Molecular Diagnostics for Use in HIV/AIDS Care and Treatment in Resource-Limited Settings |
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580 | (9) |
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42 Rapid Point-of-Care Diagnosis of Malaria and Dengue Infection |
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589 | (24) |
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Section IX THE HOST AND HOST RESPONSE |
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43 Implications of Pharmacogenetics for Antimicrobial Prescribing |
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613 | (21) |
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44 Exploiting MicroRNA (miRNA) Profiles for Diagnostics |
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634 | (21) |
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45 Host Response in Human Immunodeficiency Virus Infection |
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655 | (8) |
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46 Biomarkers of Gastrointestinal Host Responses to Microbial Infections |
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663 | (22) |
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Section X INFORMATION TECHNOLOGY |
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47 Point-of-Care Medical Device Connectivity: Developing World Landscape |
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685 | (7) |
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48 WHONET: Software for Surveillance of Infecting Microbes and Their Resistance to Antimicrobial Agents |
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692 | (15) |
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49 Cloud-Based Surveillance, Connectivity, and Distribution of the GeneXpert Analyzers for Diagnosis of Tuberculosis (TB) and Multiple-Drug-Resistant TB in South Africa |
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707 | (14) |
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Section XI QUALITY ASSURANCE |
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50 Molecular Method Verification |
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721 | (24) |
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51 Molecular Microbiology Test Quality Assurance and Monitoring |
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745 | (9) |
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52 Proficiency Testing and External Quality Assessment for Molecular Microbiology |
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754 | (12) |
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53 Practices of Sequencing Quality Assurance |
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766 | (18) |
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54 Verification and Validation of Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry-Based Protocols |
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784 | (15) |
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Section XII THE BUSINESS OF DIAGNOSTICS |
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55 Improved Diagnostics in Microbiology: Developing a Business Case for Hospital Administration |
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799 | (4) |
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56 Molecular Diagnostics and the Changing Legal Landscape |
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803 | (8) |
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| Index |
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