Multifunctional Hydrogels for Biomedical Applications Comprehensive resource presenting a thorough overview of the biomedical applications of hydrogels
This book provides an overview of the development and applications of the clinically relevant hydrogels that are used particularly in tissue engineering, regenerative medicine, and drug delivery. Taking a multidisciplinary approach, it goes through the material from chemistry, materials science, biology, medicine, nanotechnology, and bioengineering points of view. Sample topics covered by the three well-qualified editors include:
- The design, functions, and developments of hydrogels
- Proteins and polysaccharides that mimic extracellular matrix
- Generation and applications of supramolecular hydrogels
- Design and functions of cell encapsulation systems
Multifunctional Hydrogels for Biomedical Applications is a useful all-in-one reference work for materials scientists, polymer chemists, and bioengineers which provides a comprehensive, contemporary understanding of hydrogels and their applications targeting a wide variety of pathologies.
Preface xiii
1 Extracellular Matrix Hydrogels from Decellularized Tissues for Biological
and Biomedical Applications 1
Brendan C. Jones, Nicola Elvassore, Paolo De Coppi, and Giovanni G. Giobbe
1.1 Introduction to Hydrogels 1
1.2 Key Features and Functions of the Extracellular Matrix in Homeostasis
and Development 6
1.3 Extracellular Matrix-Based Hydrogels Derived from Decellularization of
Organs 8
1.4 Commercially Available Products 18
2 Collagen-Based Systems to Mimic the Extracellular Environment 23
Umber Cheema and Vivek Mudera
2.1 Cells in Tissues 23
2.2 Collagen in Tissues 24
2.3 Controlling Collagen Architecture 26
2.4 Engineering Collagen Scaffolds 29
2.5 Conclusions 33
3 Designing Elastin-Like Recombinamers for Therapeutic and Regenerative
Purposes 37
José Carlos Rodríguez-Cabello, Sara Escalera, Diana Juanes-Gusano, Mercedes
Santos, and Alessandra Girotti
3.1 Introduction 37
3.2 ELR-Based Hydrogels in Tissue Engineering 39
3.3 ELR-Based Hydrogels for Drug Delivery 48
3.4 Future Remarks 56
4 Enzyme-Assisted Hydrogel Formation for Tissue Engineering Applications 63
Sílvia Pérez-Rafael, Eva Ramon, and Tzanko Tzanov
4.1 Introduction 63
4.2 Enzymatically Cross-Linked Hydrogels 66
4.3 Supramolecular Enzyme-Driven Hydrogelation 75
4.4 Conclusions 81
5 Hierarchical Peptide- and Protein-Based Biomaterials: From Molecular
Structure to Directed Self-assembly and Applications 97
Yinchen Yuan, Yejiao Shi, and Helena S. Azevedo
5.1 Introduction 97
5.2 Molecular Design/Selection of Building Blocks for Hierarchical
Self-assembly 98
5.3 Hierarchical Assembly Through Environmental Manipulation 108
5.4 Techniques for the Characterization of Hierarchically Organized
Biomaterials 113
5.5 Application of Hierarchical Self-assembling Peptide- and Protein-Based
Biomaterials in Tissue Regeneration 117
5.6 Conclusions 120
6 Short Peptide Hydrogels for Biomedical Applications 127
Priyadarshi Chakraborty, Lihi Adler-Abramovich, and Ehud Gazit
6.1 Introduction 127
6.2 Short Peptide Hydrogels 128
6.3 Biomedical Applications of Short Peptide Hydrogels 129
6.4 Conclusions and Outlook 139
7 Supramolecular Assemblies of Glycopeptides as Mimics of the Extracellular
Matrix 149
Diana Soares da Costa, Alexandra Brito, Rui L. Reis, and Iva Pashkuleva
7.1 Introduction 149
7.2 Glycoproteins and Proteoglycans in the ECM 150
7.3 Design of Self-assembling Peptide--Saccharide Conjugates 151
7.4 Supramolecular Systems Generated by Interfacial Co-assembly 154
7.5 Conclusions 155
8 Supramolecular Assemblies for Cancer Diagnosis and Treatment 161
Shuang Liu and Bing Xu
8.1 Introduction 161
8.2 Cancer Diagnosis 162
8.3 Cancer Treatment 173
8.4 Future Perspectives 189
9 Polyzwitterionic Hydrogels as Wound Dressing Materials 195
Konstans Ruseva and Elena Vassileva
9.1 Polyzwitterions 195
9.2 Wound Management and Wound Dressings 197
9.3 PZIs as Dressings Materials for AcuteWounds 198
9.4 PZI as Dressings for Chronic Wounds Management 206
9.5 Conclusions 212
10 Hyaluronan-Based Hydrogels as Modulators of Cellular Behavior 217
Sara Amorim, Rui L. Reis, and Ricardo A. Pires
10.1 Introduction 217
10.2 Biological Relevance of Hyaluronan 218
10.3 Hyaluronan-Based Systems for Biomedical Applications 220
10.4 Conclusion and Future Remarks 226
11 Hydrogel Fibers Produced via Microfluidics 233
Kongchang Wei, Claudio Toncelli, René M. Rossi, and Luciano F. Boesel
11.1 Introduction to Microfluidics and Microfluidic Wet Spinning 233
11.2 Fabrication of Chips for Microfluidic Wet Spinning 237
11.3 Biomedical Applications of Hydrogel Fibers Produced via Microfluidics
242
11.4 Hydrogel Optical Fibers 257
11.5 Conclusions 263
12 Embedding Hydrogels into Microfluidic Chips: Vascular Transport Analyses
and Drug Delivery Optimization 275
Ana M. Martins, Alexander B. Cook, Martina Di Francesco, Maria Grazia
Barbato, Sayanti Brahmachari, Martina Pannuzzo, and Paolo Decuzzi
12.1 Introduction: Microfluidic Chips for Modeling Human Diseases and
Developing New Therapies 275
12.2 Hydrogels to Mimic the Extracellular Matrix (ECM) 276
12.3 Fabrication of Microfluidic Chips 277
12.4 Applications of Microfluidic Chips in Biophysical Transport Analysis
282
12.5 Nanoparticle Transport Analyses 284
12.6 Computer Simulations of Nanoparticle and Cell Transport 285
12.7 Conclusions and Future Directions 287
13 Multifunctional Granular Hydrogels for Tissue-Specific Repair 295
Rui J. Almeida, Ana Fernandes, Vítor M. Gaspar, and João F. Mano
13.1 Introduction 295
13.2 Granular Hydrogels -- Functional Features and Design 297
13.3 Granular Hydrogels for Tissue-Specific Repair 308
13.4 Conclusions and Future Perspectives 317
14 Injectable Hydrogels as a Stem Cell Delivery Platform for Wound Healing
323
Qian Xu, Sigen A., and Wenxin Wang
14.1 Wound Healing 323
14.2 Stem Cells for Skin Wound Healing 328
14.3 Injectable Hydrogel Dressing as a Delivery Platform 331
Index 357
Ricardo A. Pires has a background in materials engineering. Currently, he is an Assistant Researcher at 3Bs (Biomaterials, Biodegradables and Biomimetics) Research Group at the University of Minho, Braga/Guimarães, Portugal, and the European Institute of Excellence on Tissue Engineering and Regenerative Medicine at the University of Minho. He is also a member of the Portuguese Government Associate Laboratory ICVS/3Bs. His research interests include supramolecular hydrogels, 3D disease models, pathological peptide/protein aggregation, and bionanomaterials.
Iva Pashkuleva has a background in chemistry. Currently, she is a Principal Researcher at 3Bs (Biomaterials, Biodegradables and Biomimetics) Research Group at University of Minho, Braga/Guimarães, Portugal, and the European Institute of Excellence on Tissue Engineering and Regenerative Medicine at University of Minho. She is also a member of the Portuguese Government Associate Laboratory ICVS/3Bs. Her research interests include glycan supramolecular systems, self-assembly, and cell-surface interactions.
Rui L. Reis is a world recognized expert in Tissue Engineering and Regenerative Medicine (TERM). He is the Director of 3Bs (Biomaterials, Biodegradables and Biomimetics) Research Group and Full Professor of Tissue Engineering, Regenerative Medicine and Stem Cells at the University of Minho, Braga/Guimarães, Portugal. He is the CEO of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine and Director of the Portuguese Government Associate Laboratory ICVS/3Bs. He is well known for his pioneer works on the use of natural polymers for TERM.
