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Organoids: Models for Development and Disease [Kõva köide]

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  • Formaat: Hardback, 245 pages, kõrgus x laius: 254x178 mm, 11 Illustrations, black and white; Approx. 245 p. 11 illus. With online files/update., 1 Hardback
  • Sari: Methods in Molecular Biology 2951
  • Ilmumisaeg: 12-Sep-2025
  • Kirjastus: Springer-Verlag New York Inc.
  • ISBN-10: 1071646818
  • ISBN-13: 9781071646816
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  • Formaat: Hardback, 245 pages, kõrgus x laius: 254x178 mm, 11 Illustrations, black and white; Approx. 245 p. 11 illus. With online files/update., 1 Hardback
  • Sari: Methods in Molecular Biology 2951
  • Ilmumisaeg: 12-Sep-2025
  • Kirjastus: Springer-Verlag New York Inc.
  • ISBN-10: 1071646818
  • ISBN-13: 9781071646816

This volume explores the generation and characterization of organoids from different tissues, as these structures allow us to investigate questions about cell commitment and differentiation. Whether pursuing cell development or the genesis or treatment of diseases, organoid research, through the methods and techniques in this collection, aims to provide scientists with a way to regenerate different tissues or organs in their original form and function. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Organoids: Models for Development and Disease serves as an ideal guide for researchers investigating this vital area of study.

Microfluidic Chip Fabrication for Tumor Cell 3D Culture Based on
Microwell Arrays.- A Simplified and Robust Immunofluorescence Labeling Method
for Complex 3D Cell Cultures: Minimizing Manipulation and Maximizing Data in
Whole-Mount Analysis of Organoids, Spheroids and Co-Culture Models.- A
Co-Culture System for Modeling Intestinal Epithelial-Fibroblast Crosstalk.-
Paraffin Embedding and Histological Staining of Intestinal Organoids.-
Organoid Culture of Different Intestinal Segments from Human and Mouse.-
Expansion and Maturation of Innate Lymphoid Cell Precursors Using Human
iPSC-Derived Intestinal Organoids.- Mouse Small Intestinal Organoid
Cultures.- Establishing Neural Organoid Cultures for Investigating the
Effects of Microgravity in Low-Earth Orbit (LEO).- Production of
Neuroepithelial Organoids from Human Induced Pluripotent Stem Cells for
Mimicking Early Neural Tube Development.- Methods for Generating
Self-Organizing Human Patterned Heart Organoids Using Pluripotent Stem
Cells.- Generating Neuroimmune Assembloids Using Human Induced Pluripotent
Stem Cell (iPSC)-Derived Cortical Organoids and Microglia.- Generation of
Retinal Organoids Using Human Induced Pluripotent Stem Cells.-
Transplantation of Islet Organoids into Brown Adipose Tissue in a Diabetic
Mouse Model.- Spontaneous Efficient Differentiation of Human Pluripotent Stem
Cells (hPSC) upon Co-Culture of hPSCs with Human Neonatal Foreskin
Fibroblasts in 3D.- Neuromuscular Organoids to Study Spinal Cord Development
and Disease.- A Robust Human Liver Organoid Model of Hepatitis B Virus
Infection.- Establishment of Patient-Derived Organoids from Colorectal Cancer
Resection Samples.- Complex Spheroids as an Alternative: In Vivo-Like 3D
Model for Investigating the Impact of Stromal Cells on the Radiation Response
of Tumors.- 3D Cell Culture Models as a Platform for Studying Tumor
Progression, Testing Treatment Responses, and Discovering Biomarkers.