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Parasite Genomics: Methods and Protocols 2021 ed. [Pehme köide]

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  • Formaat: Paperback / softback, 336 pages, kõrgus x laius: 254x178 mm, kaal: 662 g, 25 Illustrations, color; 12 Illustrations, black and white, 1 Paperback / softback
  • Sari: Methods in Molecular Biology 2369
  • Ilmumisaeg: 29-Jul-2022
  • Kirjastus: Humana
  • ISBN-10: 1071616838
  • ISBN-13: 9781071616833
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  • Formaat: Paperback / softback, 336 pages, kõrgus x laius: 254x178 mm, kaal: 662 g, 25 Illustrations, color; 12 Illustrations, black and white, 1 Paperback / softback
  • Sari: Methods in Molecular Biology 2369
  • Ilmumisaeg: 29-Jul-2022
  • Kirjastus: Humana
  • ISBN-10: 1071616838
  • ISBN-13: 9781071616833
Teised raamatud teemal:
This detailed book provides a comprehensive series of innovative research techniques and methodologies applied to the parasite genomics research area, all applying different approaches to analyzing parasite genomes and furthering the study of genetic complexity and the mechanisms of regulation. Beginning with chapters on novel sequencing and the bioinformatics pipeline, the volume continues by exploring diagnostic approaches using genomic tools, host-parasite interactions, as well as the genomics of parasite-derived extracellular vesicles. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Parasite Genomics: Methods and Protocols creates a detailed picture of genomic approaches for researchers seeking a better understanding of characterizing parasite nucleic acid content.
Preface v
Contributors ix
PART I NOVEL SEQUENCING AND BIOINFORMATIC PIPELINES FOR THE STUDY OF PARASITE GENOMES
1 Nanopore Long Read DNA Sequencing of Protozoan Parasites: Hybrid Genome Assembly of Trypanosoma cruzi
3(12)
Florencia Diaz-Viraque
Gonzalo Greif
Luisa Bernd
Carlos Robello
2 Chromosomes Conformation Capture Coupled with Next-Generation Sequencing (Hi-C) in Plasmodium falciparum
15(12)
Mohit Kumar Gupta
Todd Lenz
Karine G. Le Roch
3 Sequencing and Reconstructing Helminth Mitochondrial Genomes Directly from Genomic Next-Generation Sequencing Data
27(14)
Nikola Palevich
Paul Haydon Maclean
4 Automated Phylogenetic Analysis Using Best Reciprocal BLAST
41(26)
Erin R. Butterfield
James C. Abbott
Mark C. Field
Part II Diagnostic Approaches Using Genomic Tools
5 An Illumina MiSeq-Based Amplicon Sequencing Method for the Detection of Mixed Parasite Infections Using the Blastocystis SSU rRNA Gene as an Example
67(16)
Jenny G. Maloney
Nadja S. George
Aleksey Molokin
Monica Santin
6 Giardia duodenalis: Detection by Quantitative Real-Time PCR and Molecular Diversity
83(18)
Alejandro Dashti
Pamela C. Koster
David Carmena
PART III HOST-PARASITE INTERACTIONS: DECIPHERING GENE FUNCTION AND MOLECULAR PROCESSES IN PARASITES
7 Conditional Gene Deletion in Mammalian and Mosquito Stages of Plasmodium bergbei Using Dimerizable Cre Recombinase
101(20)
Priyanka Fernandes
Manon Loubens
Olivier Silvie
Sylvie Briquet
8 Coupling Auxin-Inducible Degron System with Ultrastructure Expansion Microscopy to Accelerate the Discovery of Gene Function in Toxoplasma gondii
121(18)
Nicolas Dos Santos Pacheco
Dominique Soldati-Favre
9 Genome-Wide Analysis of RNA-Protein Interactions in Plasmodium falciparum Using eCLIP-Seq
139(26)
Thomas Hollin
Steven Abel
Karine G. Le Roch
10 Transcriptional Analysis of Tightly Synchronized Plasmodium falciparum Intraerythrocytic Stages by RT-qPCR
165(22)
Nuria Casas-Vila
Anastasia K. Pickford
Harvie P. Portugaliza
Elisabet Tintd-Font
Alfred Cortes
11 Analysis of the Interaction Between Plasmodium falciparum-Infected Erythrocytes and Human Endothelial Cells Using a Laminar Flow System, Bioinformatic Tracking and Transcriptome Analysis
187(12)
Yifan Wu
Philip Bouws
Stephan Lorenzen
Iris Bruchhaus
Nahla Galal Metwally
12 Integration of Genomic and Transcriptomic Data to Elucidate Molecular Processes in Babesia divergms
199(18)
Luis Miguel Gonzalez
Elena Sevilla
Miguel Ferndndez-Garcia
Alejandro Sanchez-Flores
Estrella Montero
13 Integration of Functional Genomic, Transcriptomic, and Metabolomic Data to Identify Key Features in Genomic Expression, Metabolites, and Metabolic Pathways of Babesia divergens
217(34)
Miguel Ferndndez-Garcia
Alejandro Sanchez-Flores
Luis Miguel Gonzalez
Coral Barbas
Ma. Fernanda Rey-Stolle
Elena Sevilla
Antonia Garcia
Estrella Montero
14 Genome Analysis of Programmed DNA Elimination in Parasitic Nematodes
251(12)
Jianbin Wang
15 Helminth Microbiota Profiling Using Bacterial 16S rRNA Gene Amplicon Sequencing: From Sampling to Sequence Data Mining
263(38)
Fabio Formenti
Gabriel Rinaldi
Cinzia Cantacessi
Alba Cortes
Part IV Genomics of Parasite-Derived Extracellular Vesicles
16 Methods for the Isolation and Study of Exovesicle DNA from Trypanosomatid Parasites
301(18)
Lina Maria Orrego
Romina Romero
Antonio Osuna
Luis M. De Pablos
17 Isolation and Analysis of MicroRNAs from Extracellular Vesicles of the Parasitic Model Nematodes Nippostrongylus brasiliensis and Trichuris muris
319(14)
Ramon M. Eichenberger
Index 333