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Phage Engineering and Analysis: Methods and Protocols 2024 ed. [Kõva köide]

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  • Formaat: Hardback, 300 pages, kõrgus x laius: 254x178 mm, 45 Illustrations, color; 8 Illustrations, black and white; XI, 300 p. 53 illus., 45 illus. in color., 1 Hardback
  • Sari: Methods in Molecular Biology 2793
  • Ilmumisaeg: 26-Mar-2024
  • Kirjastus: Springer-Verlag New York Inc.
  • ISBN-10: 1071637975
  • ISBN-13: 9781071637975
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Phage Engineering and Analysis: Methods and Protocols 2024 ed.Suurem pilt
  • Formaat: Hardback, 300 pages, kõrgus x laius: 254x178 mm, 45 Illustrations, color; 8 Illustrations, black and white; XI, 300 p. 53 illus., 45 illus. in color., 1 Hardback
  • Sari: Methods in Molecular Biology 2793
  • Ilmumisaeg: 26-Mar-2024
  • Kirjastus: Springer-Verlag New York Inc.
  • ISBN-10: 1071637975
  • ISBN-13: 9781071637975
Teised raamatud teemal:
Püsilink: https://www.kriso.ee/db/9781071637975.html
Märksõnad:
This volume explores the latest developments in the study and application of phage biology. The chapters in this book are divided into five parts and cover topics such as phage display, selection, and evolution; genetic and chemical modification of phages; analyzing structures by electron microscopy; characterizing phage transcripts and proteins; and the biology of whole phages. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Phage Engineering and Analysis: Methods and Protocols is a valuable resource that will help both expert and novice researchers - from backgrounds ranging from microbiologists to biophysicists to chemical engineers - further enhance their understanding of this important and evolving field.

Protocols for Building and Producing High Diversity Peptide Phage
Display Libraries.- Generation of a Naïve Human scFv Phage Display Library
and Panning Selection.- Structure-Guided and Phage-Assisted Evolution of
Therapeutic Antibodies to Reverse On-Target Point Mutation-Mediated
Resistance.- Combining a Base Deaminase Mutator with Phage-Assisted
Evolution.- Understanding the Structural Requirements of Peptide-Protein
Interaction and Applications for Peptidomimetic Development.- Contracting the
Host Range of Bacteriophage T7 Using a Continuous Evolution
System.- Single-Cell Proteomics by Barcoded Phage-Displayed Screening via an
Integrated Microfluidic Chip.- Targeted Genome Editing of Virulent
Pseudomonas Phages using CRISPR-Cas3.- Preparation of Bioconjugates of
Chimeric M13 Phage and Gold Nanorods.- Liquid Glycan Array.- Electron
Microscopy Methods for Phage-Based Study.- Visualization of Engineered M13
Phages Bound to Bacterial Targets by Transmission Electron
Microscopy.- Purification of Single-Stranded RNA Bacteriophages and Host
Receptors for Structural Determination using Cryo-Electron
Microscopy.- Obtaining Detailed Phage Transcriptomes using
ONT-Cappable-Seq.- Multi-Disciplinary Methods for Screening Toxic Proteins
from Phages and Their Potential Molecular Targets.- CRISPRpi: Inducing and
Curing Prophage using CRISPR-Interference.- Isolation of Bacteriophages on
Actinobacteria Hosts.