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Phytochromes: Methods and Protocols [Kõva köide]

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  • Formaat: Hardback, 319 pages, kõrgus x laius: 254x178 mm, 82 Illustrations, color; 11 Illustrations, black and white; X, 319 p. 93 illus., 82 illus. in color., 1 Hardback
  • Sari: Methods in Molecular Biology 2970
  • Ilmumisaeg: 15-Oct-2025
  • Kirjastus: Springer-Verlag New York Inc.
  • ISBN-10: 1071647903
  • ISBN-13: 9781071647905
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Phytochromes: Methods and ProtocolsSuurem pilt
  • Formaat: Hardback, 319 pages, kõrgus x laius: 254x178 mm, 82 Illustrations, color; 11 Illustrations, black and white; X, 319 p. 93 illus., 82 illus. in color., 1 Hardback
  • Sari: Methods in Molecular Biology 2970
  • Ilmumisaeg: 15-Oct-2025
  • Kirjastus: Springer-Verlag New York Inc.
  • ISBN-10: 1071647903
  • ISBN-13: 9781071647905
Teised raamatud teemal:
Püsilink: https://www.kriso.ee/db/9781071647905.html

This volume summarizes the state-of-the-art methodology for the functional characterization of phytochromes in vitro and in vivo. Chapters and divided into four parts covering, phytochrome discovery, spectroscopic techniques, x-ray crystallography, x-ray crystallography, and analyzing phytochrome function in vitro and in vivo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Phytochromes: Methods and Protocols aims to be a versatile laboratory bench and computational resource that is an essential reference.

 

Discovery and Engineering of Unique Cyanobacteriochrome GAF Domains.-
Analysis of Sequence Diversity in Sub-families of Phytochrome-linked
Effectors.- Strategies for Co-expression of Bilin-producing Enzymes in
Escherichia coli.- Preparation of Phycocyanobilin (PCB) with and without
13C/15N Isotope Labelling for in vitro Phytochrome Autoassembly.- Production
of Plant Phytochromes by Combining Insect Cells and Bacterial Expression
Systems.- Overcoming the challenges of producing plant phytochromes in
Escherichia coli.- Characterizing Phytochrome Photochemistry Using UV-Visible
Spectroscopy in Host Cell Extracts and Purified Protein.- Computational
Protocols to Study Phytochromes.- Using Computational Techniques for the
Characterization of Structural Changes During the Phytochrome Photocycle.-
FTIR Spectroscopy of Red and Far-red Light Sensing Phytochromes in vitro.-
Spectroscopic Analyses of Photoconversion in Phytochromes.- Small-angle X-ray
Scattering Studies on Phytochromes.- A Simple Modification of a Commercial
Plunge-freezer to Allow Time-resolved Vitrification for Cryo-EM Analyses of
Light-activated Proteins.- Structures of Myxobacterial Phytochrome Revealed
by Cryo-EM.- Structural Analysis of Phytochrome Photoreceptors using
Hydrogen-Deuterium Exchange Coupled to Mass Spectrometry.- Encapsulation of
Phytochrome Proteins in Trehalose Matrices: Practical Considerations for the
Use in Solid-State NMR Spectroscopy.- Analyzing in vitro Autokinase Activity
of Microbial Phytochromes under well-defined Light Conditions.- Detection of
Histidine Kinase activities of Bacteriophytochromes with Phos-tag Acrylamide
Gel Electrophoresis.- Assessing the Role of PPKs in Phosphorylating
Phytochrome A using the Protoplast Transient Expression System.- A Fast and
Reliable Nuclei Extraction Method for Phytochrome Functional Analysis.-
Opto-APCs: Displaying Phytochrome B on the Surface of Living Cells for
Optogenetic Studies.- Coupling Recombinant PhyB to Cell Culture Plates and
Beads.