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Pichia pastoris: Methods and Protocols [Kõva köide]

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  • Formaat: Hardback, 712 pages, kõrgus x laius: 254x178 mm, 90 Illustrations, color; 43 Illustrations, black and white; XIV, 712 p. 133 illus., 90 illus. in color. With online files/update., 1 Hardback
  • Sari: Methods in Molecular Biology 2697
  • Ilmumisaeg: 02-Oct-2025
  • Kirjastus: Springer-Verlag New York Inc.
  • ISBN-10: 1071647784
  • ISBN-13: 9781071647783
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Pichia pastoris: Methods and ProtocolsSuurem pilt
  • Formaat: Hardback, 712 pages, kõrgus x laius: 254x178 mm, 90 Illustrations, color; 43 Illustrations, black and white; XIV, 712 p. 133 illus., 90 illus. in color. With online files/update., 1 Hardback
  • Sari: Methods in Molecular Biology 2697
  • Ilmumisaeg: 02-Oct-2025
  • Kirjastus: Springer-Verlag New York Inc.
  • ISBN-10: 1071647784
  • ISBN-13: 9781071647783
Teised raamatud teemal:
Püsilink: https://www.kriso.ee/db/9781071647783.html

This volume provides readers with a useful and comprehensive resource of laboratory protocols for working with Pichia pastoris. The chapters in this book are organized into seven parts: introduction, bioinformatics, cell and systems biology, gene expression cassettes and vectors, Pichia pastoris expression strains, upstream bioprocessing, and downstream processing and product analysis. This book covers basic and newer laboratory methods, starting from the gene and genetic construct to mature (yet reasonable) amounts of a recombinant product being produced, purified, and characterized. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Pichia pastoris: Methods and Protocols is a valuable resource for both new and experienced researchers who want to learn more about this important and developing field.

A Brief History of the Pichia pastoris Expression System.- Pichia
pastoris Genomic Resources: Accessibility, Recommendations, and
Pitfalls.- Isolation of Pichia pastoris Genomic DNA for Long-Read DNA
Sequencing and PCR Applications.- Pichia pastoris RNA-Sequencing and Data
Analysis.- Localization of Proteins in the Secretory Pathway of Pichia
pastoris using Fluorescence Microscopy.- Auxin-Induced Degradation of Target
Proteins in Pichia pastoris.- Establishing Reference Genes of Pichia pastoris
Quantitative Real-Time Polymerase Chain Reaction Analyses.- ID-MS Based
Quantitative Analysis of Metabolites in Pichia pastoris: A Step-By-Step
Protocol.- High Throughput Stationary 13C-Based Metabolic Flux Analysis of
Pichia pastoris Strains.- Determination of Biomass Elemental Composition of
Pichia pastoris.- Pichia pastoris Expression Plasmid Assembly using Type IIS
Restriction Enzymes.- Autonomously Replicating Sequence-Bearing Plasmids
Utilized in Pichia pastoris.- Overlap Extension Construction and Direct
Cloning of Loop-Out Prone DNA Expression Cassettes in Pichia pastoris:
75k--Secalin from Secale cereale (rye) as a Case Study.- In Vivo Plasmid
Assembly in NHEJ-Deficient Pichia pastoris Strains.- Protein Targeting
Signals and Strategies for Pichia pastoris.- Cloning of MF Secretion
Variants for Protein Secretion in Pichia pastoris.- Lytic Polysccharide
Monooxygenases as Reporters for Signal Peptide Evaluation in Pichia
Pastoris.- Exploting Self-Processing 2A Peptides for Multi-Gene Expression in
Pichia pastoris.- Retrofitting of AOX1 Promoter-Based Pichia pastoris
Expression Strains for Methanol-Independent Protein Production.- Selective
Cultivation of Methylotrophic Pichia pastoris Laboratory and Natural
Strains.- Transformation of Pichia pastoris by Electroportation.- High
Throughput Transformation of Chemical Competent Pichia pastoris Cells using
Lithium Acetate/Chloride and Carrier DNA.- High-Efficiency Transformation of
Integrative Libraries under Zeocin Selection in Pichia pastoris.-
High-Throughput Generation of Pichia pastoris Knock-Out Strains by using
CRISPR/Cas9.- Engineering Pichia pastoris Strains using CRISPR/Cas9
Technologies: The Basic Protocol.- Adaptive Laboratory of Pichia pastoris by
Serial Batch Cultivation.- Classical Genetics of Pichia pastoris.- Pichia
pastoris Random Mutagenesis and Screening.- Cryo-Preservation of Pichia
pastoris Strains for Long-Term Storage.- Adapting Pichia pastoris Cultivation
Media to REACH Regulations.- Fed-Batch Operational Strategies for Efficient
and Robust Pichia pastoris Cultivations Achieving High Cell Densities.- A
Guide to Fed-Batch Cultivation of Pichia pastoris on Laboratory Scale.-
Pichia pastoris Continuous Cultivations for Strain Characterization and
Continuous Biomanufacturing.- Best Practices for Protein Expression in Pichia
pastoris Combining Design of Experiments with an Automated Platform for
Parallelized Cultivation in Small-Scale Stirred Tank Bioreactors.- Kinetic
Modeling of Pichia pastoris Bioreactor Systems under Different Operating
Conditions: Batch, Fed-Batch, and Chemostat.- On-Line Monitoring and Control
of Methanol Concentration in Pichia pastoris Fermentations.- Off-Gas
Balancing for Bioprocess Monitoring Pichia pastoris Bioprocesses.- A
Single-Use Technology Platform: How to Set Up Bioprocesses with Pichia
pastoris.- A Pichia pastoris Cell-Free Protein Synthesis Platform.-
Clarification of Pichia pastoris Broth by TFF with Ceramic Membranes.- Pichia
pastoris Cell Disruption by High-Pressure Homogenization (HPH).-
Concentration and Purification of Proteins Produced by Pichia pastoris by TFF
with Ceramic Membranes.- Protein Purification Protocols for Recombinant
Enzymes Produced in Pichia pastoris.- Characterization of Low Molecular
Weight Variants (Fragmentation) in Pichia pastoris by CE-SDS.