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Plant Biotic Stress: Methods and Protocols [Kõva köide]

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  • Formaat: Hardback, 361 pages, kõrgus x laius: 254x178 mm, 59 Illustrations, color; 6 Illustrations, black and white; VIII, 361 p. 65 illus., 59 illus. in color., 1 Hardback
  • Sari: Methods in Molecular Biology 2966
  • Ilmumisaeg: 03-Oct-2025
  • Kirjastus: Springer-Verlag New York Inc.
  • ISBN-10: 1071647458
  • ISBN-13: 9781071647455
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Plant Biotic Stress: Methods and ProtocolsSuurem pilt
  • Formaat: Hardback, 361 pages, kõrgus x laius: 254x178 mm, 59 Illustrations, color; 6 Illustrations, black and white; VIII, 361 p. 65 illus., 59 illus. in color., 1 Hardback
  • Sari: Methods in Molecular Biology 2966
  • Ilmumisaeg: 03-Oct-2025
  • Kirjastus: Springer-Verlag New York Inc.
  • ISBN-10: 1071647458
  • ISBN-13: 9781071647455
Teised raamatud teemal:
Püsilink: https://www.kriso.ee/db/9781071647455.html

This volume presents a comprehensive collection of protocols and techniques commonly used in research on plant-biotic interactions. Chapters guide readers through general plant–pathogen interactions, RNA interference (RNAi) in Okra, spot inoculation of begomoviruses, natural transmission, RNA viruses, enzyme-linked immunosorbent, reverse transcription polymerase chain reaction (RT-PCR), loop-mediated isothermal amplification (RT-LAMP), northern blot hybridization, and next-generation sequencing (NGS) for in-depth analysis of viral populations and mutation patterns. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Plant Biotic Stress: Methods and Protocols aims to be comprehensive guide for researchers in the field.

Plant Biotic Stress: Tools and Techniques for Crop
Protection.- Investigating the Involvement of Target Protein in
Plant-Pathogen Interactions through Ectopic Expression in Arabidopsis A
detailed methodology.- RNA interference strategy for the development of okra
(Abelmoschus esculentus L. Moench) lines resistant to Yellow vein mosaic
virus.- Spot inoculation of begomovirus by single whitefly (Bemisia tabaci)
transmission in mungbean using microcage.- One-Step RT-LAMP: A Method for
Closed-Tube Colorimetric Detection of RNA Viruses in Plants.- Detection of
Sesame Phyllody Disease and Its Transmitting Vectors.- A method for isolation
and transfection of Arabidopsis protoplast for sucrose feeding
assay.- Twitching motility dynamics in the wilt-causing phytopathogen
Ralstonia solanacearum.- A blotting paper technique for the screening of
chickpea genotypes against dry root rot disease.- CRISPR-mediated gene
editing for inducing thermo-sensitive genic male sterility and sheath blight
resistance in rice.- A Seedling Root Dip-based Technique to Screen Chickpea
for Resistance to Fusarium Wilt Disease.- Oligo-dT Anchored cDNA SCoT: An
Innovative Approach for Deciphering Fusarium wilt Resistance-Related
Differential Gene Expression in Chickpea.- Extracellular acidification assay
to evaluate the effectiveness of antifungal agents on the pathogenicity of
Macrophomina phaseolina.-  A guide to culturing, maintenance and leaf
inoculation methods for rapid screening and quantification of Sclerotinia
sclerotiorum infection in mustard.- Differential-display reverse
transcription-PCR for unraveling the molecular mechanisms in foot rot
(Phytophthora capsici sp. nov.) tolerance in black pepper (Piper nigrum
L.).- Estimation of foliar volatiles emitted by mycorrhizal colonized
blackgram (Vigna mungo L) infested with Spodoptera litura.- Development of an
Agrobacterium tumefaciens-mediated in planta transformation strategy in
pigeonpea for resistance to Helicoverpa armigera.- CRISPR/Cas9 mediated
genome editing in the management of oriental fruit fly, Bactrocera dorsalis
(Hendel) (Tephritidae: Diptera).- A novel approach using SSR markers for
identifying and breeding Bruchid pest resistant black gram (Vigna mungo L.
Hepper) genotypes.- RNAi-based approach as a promising tool for management of
leafhopper (Amrasca biguttula biguttula) in cotton.- Germplasm evaluation in
chickpea for resistance to plant parasitic nematodes.- Novel method for rapid
screening of chickpea for combined dry root rot disease and osmotic stress.