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Plant Cell and Tissue Culture - A Tool in Biotechnology: Basics and Application 2009 ed. [Kõva köide]

  • Formaat: Hardback, 333 pages, kõrgus x laius: 235x155 mm, kaal: 682 g, 63 Tables, black and white; 153 Illustrations, black and white; IX, 333 p. 153 illus., 1 Hardback
  • Sari: Principles and Practice
  • Ilmumisaeg: 19-May-2009
  • Kirjastus: Springer-Verlag Berlin and Heidelberg GmbH & Co. K
  • ISBN-10: 3540938826
  • ISBN-13: 9783540938828
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  • Formaat: Hardback, 333 pages, kõrgus x laius: 235x155 mm, kaal: 682 g, 63 Tables, black and white; 153 Illustrations, black and white; IX, 333 p. 153 illus., 1 Hardback
  • Sari: Principles and Practice
  • Ilmumisaeg: 19-May-2009
  • Kirjastus: Springer-Verlag Berlin and Heidelberg GmbH & Co. K
  • ISBN-10: 3540938826
  • ISBN-13: 9783540938828
Presents an introduction as well as a selected survey of key advances in the fascinating field of plant cell and tissue culture as a tool in biotechnology. This book describes various basic techniques employed in leading laboratories worldwide.

This book provides a general introduction as well as a selected survey of key advances in the fascinating field of plant cell and tissue culture as a tool in biotechnology. After a detailed description of the various basic techniques employed in leading laboratories worldwide, follows an extended account of important applications in, for example, plant propagation, secondary metabolite production and gene technology. Additionally, some chapters are devoted to historical developments in this domain, metabolic aspects, nutrition, growth regulators, differentiation and the development of culture systems. The book will prove useful to both newcomers and specialists, and even "old hands" in tissue culture should find some challenging ideas to think about.

Arvustused

From the reviews:

"The approach of including several examples and description of well-illustrated protocols from available primary literature and the authors own laboratory experiences and helpful guidelines make this volume a gem for conventional tissue culture practices. To the best of our experiences, this volume is suitable for both undergraduate and postgraduate level courses in life sciences, biotechnology, plant tissue culture, plant biotechnology or plant science courses. We also recommend this volume for M.Tech., M.Phil., and PhD level courses in the above-mentioned areas and related disciplines." (S. K. Basu and A. Goyal, Applied Biochemistry and Biotechnology, November, 2009)

Introduction
1(6)
Historical Developments of Cell and Tissue Culture Techniques
7(6)
Callus Cultures
13(30)
Establishment of a Primary Culture from Explants of the Secondary Phloem of the Carrot Root
16(3)
Fermenter Cultures
19(2)
Immobilized Cell Cultures
21(1)
Nutrient Media
22(6)
Evaluation of Experiments
28(1)
Maintenance of Strains, Cryopreservation
29(2)
Some Physiological, Biochemical, and Histological Aspects
31(12)
Cell Suspension Cultures
43(8)
Methods to Establish a Cell Suspension
43(3)
Cell Population Dynamics
46(5)
Protoplast Cultures
51(10)
Production of Protoplasts
54(3)
Protoplast Fusion
57(4)
Haploid Techniques
61(14)
Application Possibilities
61(3)
Physiological and Histological Background
64(3)
Methods for Practical Application
67(3)
Haploid Plants
70(5)
Plant Propagation---Meristem Cultures, Somatic Embryogenesis
75(64)
General Remarks, and Meristem Cultures
75(8)
Protocols of Some Propagation Systems
83(8)
In vitro Propagation of Cymbidium
83(3)
Meristem Cultures of Raspberries
86(3)
In vitro Propagation of Anthurium
89(2)
Somatic Embryogenesis
91(39)
Basics of Somatic Embryogenesis
95(11)
Ontogenesis of Competent Cells
106(1)
Genetic Aspects---DNA Organization
107(6)
The Phytohormone System
113(5)
The Protein System
118(9)
Cell Cycle Studies
127(3)
Practical Application of Somatic Embryogenesis
130(4)
Artificial Seeds
134(1)
Embryo Rescue
135(4)
Some Endogenous and Exogenous Factors in Cell Culture Systems
139(22)
Endogenous Factors
140(5)
Genetic Influences
140(1)
Physiological Status of ``Mother Tissue''
140(3)
Growth Conditions of the ``Mother Plant''
143(2)
Exogenous Factors
145(13)
Growth Regulators
146(2)
Nutritional Factors
148(10)
Physical Factors
158(3)
Primary Metabolism
161(20)
Carbon Metabolism
161(15)
Nitrogen Metabolism
176(5)
Secondary Metabolism
181(46)
Introduction
181(2)
Mechanism of Production of Secondary Metabolites
183(3)
Historical Background
186(4)
Plant Cell Cultures and Pharmaceuticals, and Other Biologically Active Compounds
190(12)
Antitumor Compounds
194(5)
Anthocyanin Production
199(3)
Strategies for Improvement of Metabolite Production
202(8)
Addition of Precursors, and Biotransformations
203(2)
Immobilization of Cells
205(1)
Differentiation and Secondary Metabolite Production
206(2)
Elicitation
208(2)
Organ Cultures
210(2)
Shoot Cultures
210(1)
Root Cultures
211(1)
Genetic Engineering of Secondary Metabolites
212(3)
Membrane Transport and Accumulation of Secondary Metabolites
215(4)
Bioreactors
219(6)
Technical Aspects of Bioreactor Systems
221(4)
Prospects
225(2)
Phytohormones and Growth Regulators
227(8)
Cell Division, Cell Growth, Cell Differentiation
235(14)
Genetic Problems and Gene Technology
249(38)
Somaclonal Variations
249(9)
Ploidy Stability
249(3)
Some More Somaclonal Variations
252(6)
Gene Technology
258(29)
Transformation Techniques
258(7)
Selectable Marker Genes
265(3)
β-Glucuronidase (GUS)
268(2)
Antibiotics Resistance Genes
270(2)
Elimination of Marker Genes
272(3)
Agrobacterium-Mediated Transformation in Dicotyledonous Plants
275(7)
Agrobacterium-Mediated Transformation in Monocotyledonous Plants
282(5)
Summary of Some Physiological Aspects in the Development of Plant Cell and Tissue Culture
287(4)
Summary: Applications of Plant Cell and Tissue Culture Systems
291(4)
References 295(30)
Index 325