This volume explores analytical methods to study complex lipid mixtures from plants and algae. The chapters in this book are organized into five parts and cover topics such as basic methods of lipid isolation and analysis; mass spectrometry and NMR analysis; lipid isolation and analysis from plant tissues, cell compartments and organelles; lipid signaling, lipid-protein interactions, and imaging; and lipid databases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Plant Lipids: Methods and Protocols is a valuable guide for experienced researchers and undergraduate, graduate, and Ph.D. students. This book is also an excellent resource for novice scientists with little to no experience inlipid experiments who are interested in approaching this field experimentally.
Lipid Isolation from Plants.- Three Methods to Extract Membrane
Glycerolipids: Comparing Sensitivity to Lipase Degradation and
Yield.- Thin-Layer Chromatography.- Lipid Analysis by Gas Chromatography and
Gas Chromatography Mass Spectrometry.- 14C-Tracing of Lipid
Metabolism.- Methods of Lipid Analyses for Microalgae: Charophytes,
Eustigmatophytes, and Euglenophytes.- Direct Infusion Mass Spectrometry for
Complex Lipid Analysis.- Fatty Acid Composition by
Total-Acyl-Lipid--Collision Induced Dissociation--Time-of-Flight
(TAL-CID-TOF) Mass Spectrometry.- Targeted Analysis of the Plant Lipidome by
UPLC-NanoESI-MS/MS.- Mass Spectrometry-Based Profiling of Plant Sphingolipids
from Typical and Aberrant Metabolism.- Analysis of Free and Esterified Sterol
Content and Composition in Seeds using GC and ESI-MS/MS.- Techniques for the
Measurement of Molecular Species of Acyl-CoA in Plants and
Microalgae.- Quantification of Acyl-Acyl Carrier Proteins for Fatty Acid
Synthesis using LC-MS/MS.- Structural Analysis of Glycosylglycerolipids using
NMR Spectroscopy.- Analysis of Extracellular Cell Wall Lipids: Wax, Cutin and
Suberin in Leaves, Roots, Fruits, and Seeds.- Isolation of Lipid Droplets for
Protein and Lipid Analysis.- Isolation of Plastoglobules for Lipid
Analyses.- Isolation of Mitochondria for Lipid Analysis.- Collection and
Analysis of Phloem Lipids.- Analyses of Inositol Phosphates and
Phosphoinositides by Strong Anion Exchange (SAX)-HPLC.- Analysis of
Phosphoinositides from Complex Plant Samples by Solid-Phase Adsorption
Chromatography and Subsequent Quantification via Thin-Layer and Gas
Chromatography.- Studying Lipid-Protein Interactions Using
Protein-Lipid-Overlay and Protein-Liposome-Association
Assays.- Investigations of Lipid Binding to Acyl-CoA-Binding Proteins (ACBP)
Using Isothermal Titration Calorimetry (ITC).- In Situ Localization of Plant
Lipid Metabolites by Matrix Assisted Laser Desorption/Ionization-Mass
Spectrometry Imaging (MALDI-MSI).- Plant Lipid Databases.- Lipid Pathway
Databases with a Focus on Algae.
