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Plant Long Non-Coding RNAs: Methods and Protocols 2019 ed. [Kõva köide]

  • Formaat: Hardback, 480 pages, kõrgus x laius: 254x178 mm, kaal: 1148 g, 67 Illustrations, color; 11 Illustrations, black and white; XVII, 480 p. 78 illus., 67 illus. in color. With online files/update., 1 Hardback
  • Sari: Methods in Molecular Biology 1933
  • Ilmumisaeg: 03-Apr-2019
  • Kirjastus: Humana Press Inc.
  • ISBN-10: 1493990446
  • ISBN-13: 9781493990443
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  • Formaat: Hardback, 480 pages, kõrgus x laius: 254x178 mm, kaal: 1148 g, 67 Illustrations, color; 11 Illustrations, black and white; XVII, 480 p. 78 illus., 67 illus. in color. With online files/update., 1 Hardback
  • Sari: Methods in Molecular Biology 1933
  • Ilmumisaeg: 03-Apr-2019
  • Kirjastus: Humana Press Inc.
  • ISBN-10: 1493990446
  • ISBN-13: 9781493990443

This volume focuses on various approaches to studying long non-coding RNAs (lncRNAs), including techniques for finding lncRNAs, localization, and observing their functions. The chapters in this book cover how to catalog lncRNAs in various plant species; determining subcellular localization; protein interactions; structures; and RNA modifications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and innovative, Plant Long Non-Coding RNAs: Methods and Protocols is a valuable resource that aids researchers in understanding the functions of lncRNAs in different plant species, and helps them explore currently uncharted facets of plant biology.

An Overview of Methodologies in Studying lncRNAs in the High-Throughput
Era: When Acronyms ATTACK!.- Analysis of siRNA Precursors Generated by RNA
Polymerase IV and RNA-Dependent RNA Polymerase 2 in Arabidopsis.-
Identification of Long Non-Coding RNAs in the Developing Endosperm of
Maize.- RNA Isolation and Analysis of lncRNAs from Gametophytes of
Maize.- Improved Method of RNA Isolation from Laser Capture Microdissection
(LCM) Derived-Plant Tissues.- Medium Throughput RNA In Situ Hybridization of
Serial Sections from Paraffin-Embedded Tissue Microarrays.- Purification and
Functional Analysis of Plant Long Non-Coding RNAs (lncRNA).- The Involvement
of Long Non-Coding RNAs in Response to Plant Stress.- Subcellular
Localization and Functions of Plant lncRNAs in Drought and Salt Stress
Tolerance.- Discovery, Identification, and Functional Characterization of
Plant Long Intergenic Non-Coding RNAs after Virus Infection.- Bioinformatics
Approachesto Studying Plant Long Non-Coding RNAs (lncRNAs): Identification
and Functional Interpretation of lncRNAs from RNA-seq Data
Sets.- Identification of Novel lincRNA and Co-Expression Network Analysis
using RNA Sequencing Data in Plants.- An Easy-to-Follow Pipeline for Long
Non-Coding RNA Identification: A Case Study in Diploid Strawberry Fragaria
vesca.- Reference-Based Identification of Long Non-Coding RNAs in Plants with
Strand-Specific RNA-Sequencing Data.- NAMS: Non-Coding Assessment in
Magnoliophyta Species.- De novo Plant Transcriptome Assembly and Annotation
using Illumina RNA-seq Reads.- Identification of Long Non-Coding RNA-Protein
Interactions through In Vitro RNA Pull-Down Assay with Plant Nuclear
Extracts.- Analysis of Interaction between Long Non-Coding RNAs and Protein
by RNA-Immunoprecipitation in Arabidopsis.- Trimolecular Fluorescence
Complementation (TriFC) Assay for Visualization of RNA-Protein Interaction in
Plants.- In Vivo Genome-Wide RNA Structure Probing with
Structure-seq2.- Using Protein Interaction Profile Sequencing (PIP-seq) to
Identify RNA Secondary Structure and RNA-Protein Interaction Sites of Long
Non-Coding RNAs in Plants.- Computationally Characterizing Protein Bound Long
Non-Coding RNAs and their Secondary Structure using Protein Interaction
Profile Sequencing (PIP-seq) in Plants.- Stalking Structure in Plant Long
Non-Coding RNAs.- Transcriptome-Wide Mapping 5-methylcytosine by m5C RNA
Immunoprecipitation Followed by Deep Sequencing in Plant.- A Walkthrough to
the Use of GreeNC: The Plant lncRNA Database.- CANTATAdb 2.0: Expanding the
Collection of Plant Long Non-Coding RNAs.- Experimentally Validated Plant
lncRNAs in EVLncRNAs Database.- In Situ Hi-C for Plants: An Improved Method
to Detect Long-Range Chromatin Interactions.