Polysaccharide Degrading Biocatalysts provides a thorough grounding in these biocatalytic processes and their growing role in the depolymerization of polysaccharides, thus empowering researchers to discover and develop new enzyme-based approaches across pharmaceuticals, fuels and food engineering. Here, over a dozen leading experts offer a close examination of structural polysaccharides, genetic modification of polysaccharides, polysaccharide degradation routes, pretreatments for enzymatic hydrolysis, hemicellulose degrading enzymes, biomass valorization processes, oligosaccharide production, and enzyme immobilization for hydrolysis of polysaccharides, among other topics and related research protocols. Final sections consider perspectives and challenges in an evolving carbohydrate-based economy.
- Describes the role of enzymes in the degradation of polysaccharides to obtain building blocks for biochemical processes
- Covers new tools for enzymatic evolution, research protocols, and process strategies that contribute to large-scale applications
- Explores the use of polysaccharide hydrolysis products in the areas of pharmaceuticals, fuels and food engineering
- Features chapter contributions from international experts
Arvustused
...important for both human health and the development of green biofuels and biomaterials. [ this book] is intended for research scientists either actively engaged in or considering the study of polysaccharide-degrading enzymes or their optimization for bioprocessing applications,. [ and] its approach and style are appropriate for students as well. The breadth of subject matter covered is impressive, ranging from mathematical modeling of enzyme-catalyzed polysaccharide hydrolysis to the future prospect of a carbohydrate-based economy. The illustrations complement the text,. A pleasant read, even for non-experts. [ It] is a thorough, yet surprisingly concise resource on a topic of interest to students and scientists working in the academic and industrial sectors. It is easy to envision using the book for a survey course on polysaccharide-hydrolyzing enzymes and their applications." --©Doodys Review Service, 2023, Peter J. Kennelly, PhD (Virginia Tech)
1. Plant cell wall polysaccharides: Methodologies for compositional, structural, and physicochemical characterization
2. Genetic modification of plants to increase the saccharification of lignocellulose
3. The diversity of plant carbohydrate hydrolysis in nature and technology
4. State-of-the-art experimental and computational approaches to investigate structure, substrate recognition, and catalytic mechanism of enzymes
5. Pretreatments as a key for enzymatic hydrolysis of lignocellulosic biomass
6. Importance of accessory enzymes in hemicellulose degradation
7. How ligninolytic enzymes can help in the degradation of biomass polysaccharides, cleavage, and catalytic mechanisms?
8. Biochemical and biotechnological aspects of microbial amylases
9. Hydrolysis of complex pectin structures: Biocatalysis and bioproducts
10. Macroalgal polysaccharides: Biocatalysts in biofuel/bioenergy production
11. Mathematical modeling of the enzymatic hydrolysis of polysaccharides: A primer
12. Polysaccharide deconstruction products: Production of bio-based building blocks
13. Polysaccharide degradation for oligosaccharide production with nutraceutical potential for the food industry
14. Carbohydrate-active enzymes in the production of lactose-derived tagatose
15. Immobilized biocatalysts for hydrolysis of polysaccharides
16. Carbohydrate-based economy: Perspectives and challenges
Dr. Rosana Goldbeck is presently Associate Professor in the Food Engineering Faculty (FEA) at the University of Campinas (UNICAMP). She graduated in food engineering (2005) at the Federal University of Rio Grande (FURG), with a masters degree (2008) and PhD (2012) in food engineering from the University of Campinas (UNICAMP), and a part of her PhD was carried out at the Universitat Autònoma de Barcelona (UAB). She held a postdoctoral degree at the Brazilian Laboratory of Bioethanol Science and Technology (CTBE) at the National Center for Research in Energy and Materials (CNPEM). Her experience is mainly in the area of biotechnology and bioengineering with emphasis in fermentative processes, production and purification of enzymes, cloning and expression of heterologous proteins, enzymatic hydrolysis, production of biofuels (bioethanol), and other value-added products (xylooligosaccharides) from agro-industrial residues. Dr. Patrícia Poletto is Full Research Professor at the Federal University of Santa Catarina in FlorianópolisBrazil, and a collaborating researcher at the Biological Engineering Laboratory focused on fermentative and enzymatic processes. Patrícia completed her bachelors degree in food engineering (2007) and her PhD in biotechnology (2015) in the area of production and recovery of by solid-state fermentation. She has been working on chemical, fermentative, and enzymatic methods to convert agro-industrial residues mainly into oligosaccharides.