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Practical Manual For Musculoskeletal Research, A [Kõva köide]

(Chinese Univ Of Hong Kong, Hong Kong), (Chinese Univ Of Hong Kong, Hong Kong), (Chinese Univ Of Hong Kong, Hong Kong)
  • Formaat: Hardback, 940 pages
  • Ilmumisaeg: 19-Aug-2008
  • Kirjastus: World Scientific Publishing Co Pte Ltd
  • ISBN-10: 9812706100
  • ISBN-13: 9789812706102
Teised raamatud teemal:
  • Formaat: Hardback, 940 pages
  • Ilmumisaeg: 19-Aug-2008
  • Kirjastus: World Scientific Publishing Co Pte Ltd
  • ISBN-10: 9812706100
  • ISBN-13: 9789812706102
Teised raamatud teemal:
This manual provides technical protocols for musculoskeletal research on a translational basis, i.e. a disease-orientated approach. It offers guidance on various laboratory techniques, including cell culture and molecular biology, histology and histomorphometry, microscopy and bioimaging, laboratory animal models, CT- and MRI-based densitometry and microarchitectural analysis, biomechanics and functional analysis of orthopedic kinesiology, etc. The content is simple and straightforward, with illustrations and step-by-step procedures as an easy experimental reference for personnel in basic and clinical musculoskeletal research and education. This book will provide a unique multidisciplinary platform for various professions not only orthopedics, but also biomedical engineering and biomaterial sciences involving both basic and clinical medicine.
Foreword v
Qian Chen
Shu-Xun Hou ix
Stephen M. Perren xi
L. K. Hung xiii
Preface xv
List of Contributors
xxvii
Part I Cell Culture and Molecular Biology
1(176)
DNA Microarray
3(18)
Xiao-Ling Zhang
Yan-Zhi Du
Visualizing Gene Products: Immunohistochemistry, in situ Hybridization, and Staining for β-Galactosidase Activity
21(18)
Yong-Bo Lu
Yi-Xia Xie
Jian-Quan Feng
Mesenchymal Stem Cell Culture, Expansion, and Osteogenic and Adipogenic Differentiation
39(24)
Hui Sheng
Ling Qin
Ge Zhang
Wei-Fang Jin
Jian-Jun Cao
Hong-Fu Wang
Yi-Xiang Wang
Wen-Song Tan
Stem Cells and Their Role in Bone Formation and Regeneration
63(12)
Yi-Zhi Meng
Yi-Xian Qin
Stem Cells and Tissue Engineering Applications in the Musculoskeletal System
75(24)
Chang-Hun Lee
Gregory Yourek
Eduardo Moioli
Paul A. Clark
Jeremy Jian Mao
Osteoblast Culture and Pharmacological Evaluation in vitro
99(14)
Hong-Fu Wang
Wei-Fang Jin
Jian-Jun Cao
Hui Sheng
Osteoclast Culture and Pharmacological Evaluation in vitro
113(14)
Jian-Jun Cao
Wei-Fang Jin
Hong-Fu Wang
Hui Sheng
Primary Cultures of Human Periosteal Cells
127(8)
Wing-Hoi Cheung
Wing-Sze Lee
C. Zhang
Kwok-Sui Leung
Visualization of Osteocytes and Mineralization
135(12)
Yi-Xia Xie
Ling Ye
Shu-Bin Zhang
Vladimir Dusevich
Jian-Quan Feng
Tissue Culture of Giant Cell Tumor of Bone
147(6)
Lin Huang
Ming-Hao Zheng
Chondrocyte Mechanotransduction in Three-Dimensional Cell Culture
153(12)
Xu Yang
Riaz Gillani
Qian Chen
Chondrocyte-Pellet Culture for Cartilage Repair Research
165(12)
Wing-Hoi Cheung
Kwoon-Ho Chow
Kwong-Man Lee
Kwok-Sui Leung
Part II Histology and Histomorphometry
177(100)
Tissue Preparations
179(22)
Yong-Bo Lu
Yi-Xia Xie
Jian-Quan Feng
Acceleration of Bone Decalcification by Ultrasound
201(18)
Xia Guo
Wai-Ling Lam
Stains of Bone and Cartilage
219(12)
Yong-Bo Lu
Yi-Xia Xie
Jian-Quan Feng
Contact Microradiography for Studying the Degree of Bone Mineralization
231(18)
Yong-Ping Cao
Tasuku Mashiba
Xin Yang
Chao Liu
Satoshi Mori
Undecalcified Histology in Studying Hard Tissue Implanted with Calcium Phosphate-based Ceramics
249(28)
Chun-Wai Chan
Ling Qin
Part III Microscopy and Bioimaging
277(70)
Protocols of Micro-Computed Tomographic Analysis Established for Musculoskeletal Applications
279(22)
Hiu-Yan Yeung
Kwok-Sui Leung
Jack Chun-Yiu Cheng
Po-Yee Lui
Ge Zhang
Ling Qin
Microangiography for Studying Neovascularization During Long Bone Fracture Repair in a Rat Model
301(12)
Xiao-Zhang Zhou
Ge Zhang
Qi-Rong Dong
Ling Qin
High-Resolution Imaging of Organs and Tissues by in vivo Micro-Computed Tomography
313(18)
Engin Ozcivici
Yen-Kim Luu
Clinton Rubin
Stefan Judex
Positron Emission Tomography of Bone in Small Animals
331(16)
Erik Mittra
Shahriar S. Yaghoubi
Yi-Xian Qin
Part IV Laboratory Animal Models
347(234)
Surgical Anesthesia and Analgesia for Animals in Musculoskeletal Research
369(1)
Dewi K. Rowlands
Anthony E. James
Mouse Model of Calvarial Osteolysis
369(12)
Chao Zhang
Ting-Ting Tang
Distraction Osteogenesis Model
381(20)
Chun-Wai Chan
Kwok-Sui Leung
Fracture Nonunion Animal Model
401(20)
Xia Guo
Mu-Qing Liu
Chi-Cheung Hui
Zheng Guo
Establishment of Osteoporosis Model in Goats
421(20)
Wing-Sum Sin
Ling Qin
Kwok-Sui Leung
Posterior Spinal Fusion Model
441(16)
Chun-Wai Chan
Jack Chun-Yiu Cheng
Functional Disuse Model for Musculoskeletal Adaptation
457(20)
Ho-Yan Lam
Yi-Xian Qin
Neurogenic Limb Disuse Animal Models
477(18)
Xia Guo
Xiao-Yun Wang
Wai-Ling Lam
Establishment of Steroid-Associated Osteonecrosis Rabbit Model
495(16)
Ge Zhang
Ling Qin
Hui Sheng
Establishment of Anterior Cruciate Ligament Reconstruction Model in Rabbit
511(24)
Chun-Yi Wen
Kai-Ming Chan
Establishment of Normal and Delayed Bone-Tendon Junction Repair Models
535(24)
Kwok-Sui Leung
Ling Qin
Anterior Cruciate Ligament Transection (ACLT)-Induced Osteoarthritis in Rats
559(10)
Ya-Feng Zhang
Jun-Fei Wang
Ge Zhang
Establishment of Rabbit Partial Growth Plate Defect Model
569(12)
Kwoon-Ho Chow
Ngai-Man Cheung
Wing-Hoi Cheung
Kwok-Sui Leung
Part V X-Ray- and MRI-based Densitometry
581(190)
Micro-CT 3D Image Analysis Techniques for Orthopedic Applications: Metal Implant-to-Bone Contact Surface and Porosity of Biomaterials
583(22)
Phil Salmon
Application of DXA to Assess Orthopedic Implants
605(14)
Tom V. Sanchez
Jing-Mei Wang
Clinical Monitoring of Bone Mineralization in Distraction Osteogenesis Using DXA
619(16)
Vivian Wing-Yin Hung
Bobby Kin-Wah Ng
Jack Chun-Yiu Cheng
In vivo and ex vivo Bone Mineral Density and Structure Measurements Using XtremeCTR --- A High-Resolution pQCT (HRpQCT)
635(14)
Maurus Neff
Helmut R. Radspieler
Ling Qin
Maximilian A. Dambacher
Advanced 3D Image Processing Methods for Quantifying Proximal Femur and Vertebra Structures from QCT Images
649(22)
Wen-Jun Li
Ying Lu
Thomas Lang
Micro-Finite Element Analysis of Bone
671(20)
He Gong
Ming Zhang
Ling Qin
The Characterization of Cortical Bone Water Distribution and Structure Changes on Age, Microdamage, and Disuse by Nuclear Magnetic Resonance
691(38)
Qing-Wen Ni
Daniel P. Nicolella
Xiao-Du Wang
Jeffry S. Nyman
Yi-Xian Qin
Dynamic Contrast-Enhanced Magnetic Resonance Imaging of the Musculoskeletal System: Basic Principles and Clinical Applications in Bone Sarcomas and Rheumatoid Arthritis
729(20)
Yi-Xiang Wang
Noninvasive Evaluation of Knee Cartilage Morphology by Magnetic Resonance Imaging
749(22)
Yi-Xiang Wang
Part VI Biomechanics and Motion Analysis
771(120)
Cell Traction Force Microscopy for Musculoskeletal Research
773(16)
James Hui-Cong Wang
Bin Li
Jeen-Shang Lin
Nanoindentation: Techniques and Technical Considerations for Musculoskeletal Research
789(24)
Suzanne Ferreri
Stefan Judex
Yi-Xian Qin
Micromechanical Testing of Bone Tissues in Tension
813(26)
Xiao-Du Wang
Michael Reyes
Xuan-Liang Dong
Hui-Jie Leng
Technical Manual for Biomechanical Testing of Musculoskeletal Tissues
839(30)
Daniel Hung-Kay Chow
Andrew D. Holmes
Ling Qin
Wing-Sum Siu
Alon Lai
Motion Analysis in Musculoskeletal Research
869(22)
Zong-Ming Li
Index 891