This detailed volume examines techniques for better understanding mammalian preimplantation embryo development, the development and contribution of gametes, and how to manipulate such reproductive cells in the laboratory. Written by experts in their field, the book covers gamete preparation, in vitro embryo culture and cryopreservation, physiological and molecular methods for embryo assessment, and emerging methods for modeling key developmental processes encompassing gamete and embryo manipulation through implantation. As part of the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.
Authoritative and practical, The Preimplantation Embryo: Methods and Protocols serves as a comprehensive and practical toolbox and will be of immense value to scientists working on the fascinating and dynamic preimplantation embryo and the gametes from which it originates.
