This edited volume provides a comprehensive guide to the effective and meaningful integration of AI technologies in science education. Designed for K-12 pre-service science teachers, it explores how AI-supported instructional practices can reshape science lessons, enhance pedagogical approaches, and redefine future learning environments. While AI in education is widely studied, few academic resources offer a holistic view of its theoretical foundations, classroom applications, and long-term implicationsparticularly in science education. Addressing this gap, this book serves as an essential reference for educators, researchers, policymakers, and content developers. It offers practical insights, evidence-based strategies, and future perspectives to equip pre-service science teachers with the knowledge and tools needed to harness AI in their classrooms, transforming teaching and learning in the digital age.