This book describes basic cell engineering methods, emphasizing stem cell applications, and use of the genetically modified stem cells in cell therapy and drug discovery. Together, the chapters introduce and offer insights on new techniques for engineering of stem cells and the delivery of transgenes into stem cells via various viral and non-viral systems. The book offers a guide to the types of manipulations currently available to create genetically engineered stem cells that suit any investigator's purpose, whether it's basic science investigation, creation of disease models and screens, or cells for therapeutic applications.
Arvustused
"This is an excellent resource on the new techniques for the engineering of stem cells and the delivery of transgenes into stem cells via various viral and nonviral systems. It provides comprehensive information on the concepts of cell engineering principles and their application in primary and stem cells. Researchers, established stem cell scientists, and graduate students interested in this field will find this book very handy." ( Doody's , 17 August 2012)
FOREWORD vii Michele Calos PREFACE ix CONTRIBUTORS xi PART I: CLONING
AND GENE DELIVERY 1
1. DNA Assembly Technologies Based on Homologous
Recombination 3 Billyana Tsvetanova, Lansha Peng, Xiquan Liang, Ke Li,
Jian-Ping Yang, Tony Ho, Josh Shirley, Liewei Xu, Jason Potter, Wieslaw
Kudlicki, Todd Peterson, and Federico Katzen
2. Multigene Assembly for
Construction of Synthetic Operons: Creation and Delivery of an Optimized
All-IN-One Expression Construct for Generating Mouse iPS Cells 19 Takefumi
Sone, Yoko Takata, Hiroe Kishine, Taichi Andoh, Fumio Imamoto, and Jonathan
D. Chesnut
3. Strategies for the Delivery of Naked DNA 37 Jennifer C. Moore
and Ronald P. Hart PART II: NONINTEGRATING TECHNOLOGIES 49
4. Episomal
Vectors 51 Junying Yu
5. Nonintegrating DNA Virus 85 Chris Kemp and David
Kuninger
6. Nonintegrating RNA Viruses 103 Noemi Fusaki
7. Protein
Delivery 119 Xiao-Jian Han and Kazuhito Tomizawa PART III: INTEGRATING
TECHNOLOGIES 135
8. Sleeping Beauty Transposon-Mediated Stable Gene
Delivery 137 Andrew Wilber, Xianzheng Zhou, Xin Huang, Dan S. Kaufman, and
R. Scott McIvor
9. Integrating Viral Vectors for Gene Modifications 163
Gemma L. Mendel, Brian Paszkiet, and Rafal P. Witek
10. Bacteriophage
Integrases for Site-Specific Integration 199 Bhaskar Thyagarajan
11.
Improving Gene Targeting Efficiency in Human Pluripotent Stem Cells 211
Sangyoon Han, Anna McCann, Louise C. Laurent, Jeanne F. Loring, and Ying Liu
PART IV: APPLICATIONS 227
12. Modified Stem Cells as Disease Models and in
Toxicology Screening 229 Catharina EllerstrEUROom, Peter Sartipy, Raimund
Strehl, and Johan Hyllner
13. Screening and Drug Discovery 251 Elizabeth A.
Davenport and Robert S. Ames INDEX 273
UMA LAKSHMIPATHY is a principal investigator at Life Technologies. She has a PhD in life sciences, with academic and industry experience in molecular biology and stem cells. Dr. Lakshmipathy holds four patents and has authored more than forty publications. BHASKAR THYAGARAJAN is a program manager at Life Technologies. He has a PhD in pharmacology, with expertise in the areas of molecular biology, DNA recombination, gene and cell therapy, and protein purification. He holds one patent and has authored more than twenty publications.
