This book is exclusively structured around chromatography techniques and is unique in the market for catering to these needs. It provides a complete description of experimental methodologies in high-performance liquid chromatography (HPLC), gas chromatography (GC), ion chromatography (IC), and paper chromatography. Numericals are provided along with solutions at the end of chapters. A complete theoretical description, along with all necessary equations, is included in this book. Techniques such as paper chromatography, gel permeation chromatography, thin-layer chromatography, liquid-liquid partition chromatography, adsorption chromatography, and column chromatography are discussed in a lucid manner, and their practical utilities are properly addressed. Instrumentation related to HPLC, GC, and ion chromatography is discussed with diagrams in a comprehensible fashion. Column chemistry in relation to the mobile phase is specially mentioned so that students and lab technicians engaged in laboratory workflows can benefit. Special reference to pesticide residue is made for each chromatographic technique, which will benefit food testing laboratories. Method optimization for both HPLC and GC is detailed, providing a ready reference for method development. Method validation for both HPLC and GC, with Excel spreadsheets, has been properly incorporated so that students, lab technicians, and experts from the food industry and pharmaceuticals can refer to this book for a conceptual framework.
The ultimate goal of this book is to assist learners, including students, food analysts, and pharmaceutical technicians, in understanding method development, method validation, and troubleshooting with practical examples and proper experimental layouts. The content and treatment of the subject matter are considered most appropriate for university students studying chromatography who wish to learn how to use data for various parameters such as linearity, repeatability, limit of quantitation (LOQ), and limit of detection (LOD). True data are illustrated in Excel spreadsheets, and solutions are provided in a stepwise manner to ensure that all stakeholders can properly grasp the concepts. Concepts such as theoretical plates, plate height, resolution, selectivity, and the Van Deemter equation are demonstrated with practical data, and solutions are addressed in a simplistic manner. The ICH (International Consortium for Harmonization), SANTE guidelines, and other regulatory compliances have been intentionally incorporated to facilitate the NABL accreditation of laboratories across the country. Measures of Uncertainty (MoU) are given full attention and exemplified in Excel datasheets so that all PG students and other personnel can become acquainted with the methodology used in elucidating the MoU.
