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Protein Arrays: Methods and Protocols [Kõva köide]

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  • Formaat: Hardback, 240 pages, kõrgus x laius: 254x178 mm, 43 Illustrations, color; 4 Illustrations, black and white; XI, 240 p. 47 illus., 43 illus. in color., 1 Hardback
  • Sari: Methods in Molecular Biology 2929
  • Ilmumisaeg: 03-Jul-2025
  • Kirjastus: Springer-Verlag New York Inc.
  • ISBN-10: 1071645943
  • ISBN-13: 9781071645949
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  • Formaat: Hardback, 240 pages, kõrgus x laius: 254x178 mm, 43 Illustrations, color; 4 Illustrations, black and white; XI, 240 p. 47 illus., 43 illus. in color., 1 Hardback
  • Sari: Methods in Molecular Biology 2929
  • Ilmumisaeg: 03-Jul-2025
  • Kirjastus: Springer-Verlag New York Inc.
  • ISBN-10: 1071645943
  • ISBN-13: 9781071645949
Teised raamatud teemal:

This volume discusses the latest methods and applications of protein microarrays and helps researchers harness its power in their work. The chapters in this book explore protein microarray’s development and fabrication, strategies for their data analysis, and wide-reaching applications in biology and disease research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Protein Arrays: Methods and Protocols is a valuable resource for graduate students, postdoctoral researchers, laboratory scientists, and industry professionals who wish to learn more about protein microarray technology.

Chapter 3 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

 Printing High-Density Human Protein Fragments on Epoxy-Treated
Slides.- Building Reversed-Phase Microarrays on Low-Cost Biosensing Chips:
Application to Multiplexed Detection of Food Allergies.- Bead-Based
Suspension Array Using Peptides as Antigens to Capture and Identify Serum
Antibodies.-  On-Array Citrullination of Protein Microarrays.-
High-Throughput Post-Translational Modification Analyses by Reverse Phase
Protein Array.- Optimization of Serum and Microarray Concentrations for a
Semiquantitative Multiplex Indirect Immunoassay.- Protein Microarray Analysis
with GenePix.- Evaluating Cross-Reactivity in Custom-Made Multiplex
Bead-Based Antibody Microarrays: A Methodological Approach.- SomaScan
Bioinformatics: Normalization, Quality Control, and Assessment of
Pre-Analytical Variation.- Proteomics by qPCR Using Proximity Extension Assay
(PEA).- Multi-Array Technology for Secreted Cytokine Profiling in a
Nano-Aerosol Exposed 3D Human Alveolar Cell Model.- Use of Humanized
NFAT-Dsred RBL Reporter for Detection of Allergen-Specific IgE Sensitization
in Human Serum in 96-Well Microarray Format.- A Competitive Ligand-Binding
Assay for Simultaneous Detection of Neutralizing Antibodies to Both Arms of a
Bispecific Drug.- Alternative Matrices for Protein Biomarker Analysis by qPCR
using Proximity Extension Assay (PEA).- Cytokine Antibody Microarray-Based
Proteomic Strategies for Characterizing the Dysregulation of Cytokines
Disease-Specific.- Unravelling Immunogenic Cell Death Mechanisms by
Functional Proteomics Arrays.- Combination of Antibody Arrays to Characterize
the Effect of Neuropathological Stimulus in Human Olfactory Neuroepithelial
Cells.