Protein Expression in Animal Cells presents information essential to researchers and students investigating cellular function. This exceptional laboratory guide covers the selection and use of expression systems that are optimal for specific exogenous proteins. Current studies often require protein expression in particular cell types, in amounts suitable for specific detection methods, and under conditions that do not otherwise perturb the specific cellular mechanisms under investigation. Such demanding requirements imply tht there is no universal vector for all experiments.
This comprehensive manual describes the structure, production, host range, and practical use of diverse viral and plasmid vectors. Protocols are written at a level geared to the beginning lab student. Because it covers many versatile expression systems, expert researchers will also find this guidebook a valuable addition to their laboratory.
Key Features
* Describes both well-established and novel recombinant vector systems for expression of proteins
* Presents methods for efficient delivery of recombinant genes into differentiated cells, tissues, and whole animals
* Covers high-level and inducible systems, plus assays for protein expression
* Provides beginning and advanced investigators and students with the information they need to choose the optimal viral or plasmid system for their protein
* Practical, benchtop-style presentation works in lab and in the classroom
Muu info
Key Features * Describes both well-established and novel recombinant vector systems for expression of proteins * Presents methods for efficient delivery of recombinant genes into differentiated cells, tissues, and whole animals * Covers high-level and inducible systems, plus assays for protein expression * Provides beginning and advanced investigators and students with the information they need to choose the optimal viral or plasmid system for their protein * Practical, benchtop-style presentation works in lab and in the classroom
The Use of Naturally Occurring and Recombinant Viruses:
R.W. Compans and P.C. Roberts, Viruses as Model Systems in Cell Biology.
V.M. Olkkonen, P. Liljeström, P. Dupree, H. Garoff, and K. Simons, Expression
of Exogenous Proteins in Mammalian Cells with the Simliki Forest Virus
Vector.
R.C. Piper, J.W. Slot, G. Li, P.D. Stahl, and D.E. James, Recombinant Sindbis
Virus as an Expression System for Cell Biology.
G. Odorizzi and I.S. Trowbridge, Recombinant Rous Sarcoma Virus Vectors for
Avian Cells.
J.-K. Yee, T. Friedmann, and J.C. Burns, Generation of High-Titer Pseudotyped
Retroviral Vectors with Very Broad Host Range.
H.Y. Naim and M.G. Roth, SV40 Virus Expression Vectors.
O.A. Weisz and C.E. Machamer, Use of Recombinant Vaccinia Virus Vectors for
Cell Biology.
T.C. Becker, R.J. Noel, W.S. Coats, A.M. Gómez-Foix, T. Alam, R.D. Gerard,
and C.B. Newgard, Use of Recombinant Adenovirus for Metabolic Engineering of
Mammalian Cells.
D.Y. Ho, Amplicon-Based Herpes Simplex Virus Vectors.
P.A. Johnson and T. Friedmann, Replication-Defective Recombinant Herpes
Simplex Virus Vectors.
The Use of Expression Plasmids in Continuous Cell Lines:
C.B. Brewer, Cytomegalovirus Plasmid Vectors for Permanent Lines of Polarized
Epithelial Cells.
R.P. Hirt, N. Fasel, and J.-P. Kraehenbuhl, Inducible Protein Expression
Using a Glucocorticoid-Sensitive Vector.
R.A. Chavez, Y.-T. Chen, W.K. Schmidt, L. Carnell, and H.-P. Moore,
Expression of Exogenous Proteins in Cells with Regulated Secretory Pathways.
D.G. Cook, V.M.-Y. Lee, and R.W. Doms, Expression of Foreign Proteins in a
Human Neuronal System.
T.E. Willnow and J. Herz, Homologous Recombination for Gene Replacement in
Mouse Cell Lines.
T. Mattioni, J.-F. Louvion, and D. Picard, Regulation of Protein Activities
by Fusion to Steroid Binding Domains.
S.A. Johnston and D.-C. Tang, Gene Gun Transfection of Animal Cells and
Genetic Immunization.
Index.
Volumes in Series.
University of California, Santa Barbara, USA
