This volume summarizes the presentations made at the third international meeting of its kind, held in Zeist, The Netherlands. The two previous conferences had witnessed the increasing importance of brain phosphoproteins in the study of the brain. The rapid acceleration in our knowledge is evident as we progress from a period of characterizing spots on a gel to considering their physiological implications from a diversity of fields: behavioral psychology, neurophysiology, pharmacology, cellular and molecular biology, each providing a view of brain phosphoprotein function from a unique perspective. The present volume retains as its primary focus the function of those nervous system proteins that are kinases or substrates for kinases, and reveals the first wave of research emerging from the cloning of the cDNA for both kinase and substrate. An incisive presentation focusing on protein kinase and its substrates is included in the volume and provides the basis for discussion for many of the subsequent contributions.
Preface. Participants. Section I: Structure and Characteristics of the
Growth and Plasticity Associated PKC Substrate B-50/F1/GAP-43/neuromodulin.
1. B-50: structure, processing and interaction with ACTH (H. Zwiers and P.J.
Coggins).
2. GAP-43: purification from a prokaryotic expression system,
phosphorylation in cultured neurons, and regulation of synthesis in the
central nervous system (S.K. Doster, A.M. Lozano, S.M. Shuh, S. Spencer and
M.B. Willard).
3. Selective phosphorylation and dephosphorylation of the
protein B-50 (L.A. Dokas, M.R. Pisano and Y.-F. Han).
4. Mutagenesis of the
calmodulin binding domain of neuromodulin (E.R. Chapman, D. Au, T.A. Nicolson
and D.R. Storm). Section II: Regulation of Expression of
B-50/F1/GAP-43/neuromodulin.
5. Expression of the growth- and
plasticity-associated neuronal protein, GAP-43, in PC12 pheochromocytoma
cells (B. Costello, L.-H. Lin, A. Meymandi, S. Bock, J.J. Norden and J.A.
Freeman).
6. The expression of GAP-43 in relation to neuronal growth and
plasticity: when, where, how, and why? (L.I. Benowitz and N.I.
Perrone-Bizzozero).
7. GAP-43 and neuronal remodeling (M.C. Fishman and D.
Valenzuela).
8. Regulation of gene expression in the olfactory
neuroepithelium: a neurogenetic matrix (F.L. Margolis, J. Verhaagen, S.
Biffo, F. Huang and M. Grillo). Section III: Structure and Characteristics of
Protein Kinases.
9. Protein kinase C family and nervous function (Y.
Nishizuka, M.S. Shearman, T. Oda, N. Berry, T. Shinomura, Y. Asaoka, K.
Ogita, U. Kikkawa, A. Kishimoto, A. Kose, N. Saito and C. Tanaka).
10.
Protein kinase C subtypes and their respective roles (K.-P. Huang, F.L.
Huang, C.W. Mahoney and K.-H. Chen).
11. Regional distribution and properties
of an enzyme system in rat brain that phophorylates ppH-47, an insoluble
protein highly labelled in tissue slices from the hippocampus (R. Rodnight,
C.A. Gonccalves, R. Leal, E. Rocha, C.G. Salbego and S.T. Wofchuk).
12.
Molecular and cellular studies on brain calcium/calmodulin-dependent protein
kinase II (T.R. Soderling, K. Fukunaga, D.A. Bricket, Y.L. Fong, D.P. Rich,
K. Smith and R.J. Colbran). Section IV: Plasticity and Function of the PKC
substrate B-50/F1/GAP-43/neuromodulin.
13. Protein kinase C substrate B-50
(GAP-43) and neurotransmitter release (P.N.E. de Graan, A.B. Oestreicher, P.
Schotman and L.H. Schrama).
14. Transmitter release: target of regulation by
protein kinase C? (L.V. Dekker, P.N.E. de Graan and W.H. Gispen).
15.
Activation of protein kinase C phosphorylation pathways: a role for storage
of associative memory (D.S. Lester and D.L. Alkon).
16. A tale of two
contingent protein kinase C activators: both neutral and acidic lipids
regulate synaptic plasticity and information storage (A. Routtenberg).
17.
The neuropil and GAP-43/B-50 in normally ageing and Long-term potentiation:
postsynaptic activation of Ca 2+ -dependent protein kinases with subsequent
presynaptic enhancement (R. Malinow and R.W. Tsien).
