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Protein NMR Techniques Softcover reprint of the original 3rd ed. 2012 [Pehme köide]

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  • Formaat: Paperback / softback, 518 pages, kõrgus x laius: 254x178 mm, kaal: 1355 g, XIV, 518 p., 1 Paperback / softback
  • Sari: Methods in Molecular Biology 831
  • Ilmumisaeg: 23-Aug-2016
  • Kirjastus: Humana Press Inc.
  • ISBN-10: 1493961950
  • ISBN-13: 9781493961955
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  • Formaat: Paperback / softback, 518 pages, kõrgus x laius: 254x178 mm, kaal: 1355 g, XIV, 518 p., 1 Paperback / softback
  • Sari: Methods in Molecular Biology 831
  • Ilmumisaeg: 23-Aug-2016
  • Kirjastus: Humana Press Inc.
  • ISBN-10: 1493961950
  • ISBN-13: 9781493961955
In its expanded third edition, this Methods in Molecular Biology (TM) volume offers techniques for NMR sample preparation, solution and solid state NMR methodologies and data processing, materials lists, step-by-step protocols, troubleshooting tips and more.

In its expanded third edition, this Methods in Molecular Biology™ volume offers techniques for NMR sample preparation, solution and solid state NMR methodologies and data processing, materials lists, step-by-step protocols, troubleshooting tips and more.

The field of protein NMR spectroscopy has rapidly expanded into new areas of biochemistry, molecular biology and cell biology research that were impossible to study as recently as ten years ago. This third edition of Protein NMR Techniques, expands upon the previous editions with current, detailed authoritative but down-to-earth descriptions of new methodologies. These include techniques for NMR sample preparation, solution and solid state NMR methodologies and data processing. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Protein NMR Techniques,Third Edition, seeks to aid scientists in understanding the latest innovations in the field of protein NMR.

A Novel Bacterial Expression Method with Optimized Parameters for Very
High Yield Production of Triple-labeled Proteins.- Isotopic Labeling of
Heterologous Proteins in the Yeast Pichia pastoris and Kluyveromyces lactis.-
Isotope Labeling in Insect Cells.- Isotope Labeling in Mammalian Cells.-
Cell-free Protein Production for NMR Studies.- Cell-free Membrane Protein
Expression for Solid-state NMR.- Expression and Purification of Src-family
Kinases for Solution NMR Studies.- NMR Studies of Large Protein Systems.-
Protein Dynamics by 15N Nuclear Magnetic Relaxation.- Bacterial Production
and Solution NMR Studies of a Viral Membrane Ion Channel.- Preparation of the
Modular Multi-Domain Protein RPA for Study by NMR Spectroscopy.- NMR Studies
of Protein-RNA Interactions.- Preparation and Optimization of Protein-DNA
Complexes Suitable for Detailed NMR Studies.- NMR Studies of Protein-ligand
Interactions.- In-cell NMR Spectroscopy in Escherichia coli.- Deuterated
Peptides and Proteins: Structure and Dynamics Studies by MAS solid-state
NMR.- Solid-State NMR Spectroscopy of Protein Complexes.- Synthesis,
Purification and Characterization of Single Helix Membrane Peptides and
Proteins for NMR Spectroscopy.- Assignment of Backbone Resonances in a
Eukaryotic Protein Kinase ERK2.- Electrostatics of Hydrogen Exchange for
Analyzing Protein Flexibility.- Fast Protein Backbone NMR Resonance
Assignment using the BATCH Strategy.- Comprehensive Automation for NMR
Structure Determination of Proteins.- ARIA for Solutionand Solid-state NMR
Protein NMR Techniques.- Determining Protein Dynamics from 15N Relaxation
Data by using DYNAMICS.