Still the only concise practical guide, this edition combines the best features of a laboratory manual with a concise reference for successful experimental design. Full coverage of electrophoresis, liquid chromatography, mass spectrometry and further relevant techniques.
Still the only concise practical guide to laboratory experiments in proteomics, this new edition now also covers DIGE technology and liquid-chromatography, while the troubleshooting section has been considerably extended. Adopting a practical approach, the authors present the relevant techniques and explain the route to successful experimental design and optimal method selection. They cover such electrophoretic techniques as isoelectric focusing, SDS page, 2-D page, and DIGE, as well as liquid-chromatography techniques, such as ion exchange, affinity chromatography and reversed-phase HPLC. Mass-spectrometric techniques include MALDI, ESI, and FT ICR.Generously illustrated, partly in color, the book also features updates of protocols as well as animations illustrating crucial methodological steps on a companion website.
PrefaceForewordAbbreviationsGlossary of termsINTRODUCTION HistoryCritical pointsHypothesis driven approach vs. High Throughput Complementarities of workflowsConcept of experimental planningSuccess stories, highlights of useful referencesPART I: PROTEOMICS TECHNOLOGYElectrophoresisLiquid ChromatographyMass spectrometryBioinformatics tools / data miningMethods under developmentPART II. PRACTICAL MANUAL OF PROTEOME ANALYSISStrategy where to startGel-based workflowLC-based workflowLatest trendsValidation with Western blottingStatistic evaluationPART III: TROUBLE SHOOTINGGel-based workflowLC based workflowReferencesIndex
"This book will be a valuable addition to any laboratory engaged in proteomics and is highly recommended." ( Internet Journal of Genomics and Proteomics 2008)
