| Preface |
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xii | |
| Acknowledgements |
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xiv | |
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1 | (48) |
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1.1. The Changing Agricultural Scene |
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1 | (10) |
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1.1.1. Species under consideration |
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2 | (5) |
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1.1.2. Consumer, social and ethical issues |
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7 | (1) |
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1.1.3. Human health considerations |
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8 | (1) |
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1.1.4. Organic livestock farming |
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9 | (2) |
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1.2. Animal Welfare Considerations |
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11 | (5) |
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1.2.1. Education and training |
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11 | (1) |
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1.2.2. Grazing and non-grazing animals |
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12 | (1) |
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1.2.3. Biological measures of animal welfare |
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13 | (1) |
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1.2.4. Animal welfare and the consumer |
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14 | (1) |
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1.2.5. Modification of management practices |
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14 | (1) |
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1.2.6. Altering animal temperament |
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15 | (1) |
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1.2.7. Stress and slaughter |
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15 | (1) |
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1.3. Current Application of Reproductive Technologies |
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16 | (12) |
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1.3.1. General considerations |
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16 | (1) |
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1.3.2. Dairy and beef cattle |
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17 | (1) |
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18 | (3) |
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21 | (2) |
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23 | (1) |
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1.3.6. Buffaloes and yaks |
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24 | (2) |
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26 | (1) |
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27 | (1) |
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28 | (1) |
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1.4. Factors Affecting Male Fertility |
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28 | (5) |
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29 | (1) |
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1.4.2. Physiological and endocrinological factors |
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30 | (2) |
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1.4.3. Genetic and environmental factors |
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32 | (1) |
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1.4.4. Tests for predicting breeding potential of males |
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32 | (1) |
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1.5. Factors Affecting Female Fertility |
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33 | (9) |
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33 | (2) |
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1.5.2 Genetic and environmental factors |
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35 | (4) |
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1.5.3 Management and nutritional considerations |
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39 | (2) |
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1.5.4 Disease and metabolic disorders |
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41 | (1) |
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1.6 Enhancing Female Fertility |
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42 | (4) |
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1.6.1. Nutritional approaches to enhanced fertility |
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42 | (1) |
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1.6.2. Hormones to enhance fertility |
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43 | (3) |
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1.6.3. Trophoblastic vesicles |
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46 | (1) |
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46 | (3) |
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1.7.1. New opportunities and fresh challenges |
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46 | (1) |
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1.7.2. Maternal recognition of pregnancy |
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47 | (1) |
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1.7.3. Nutrition and reproduction |
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48 | (1) |
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2 Artificial Insemination |
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49 | (33) |
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2.1. Advantages of Artificial Insemination |
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51 | (9) |
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2.1.1. Cattle and buffaloes |
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51 | (2) |
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53 | (2) |
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55 | (1) |
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56 | (2) |
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58 | (1) |
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59 | (1) |
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2.2. Growth and Development of AI Technology |
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60 | (15) |
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60 | (2) |
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2.2.2. Collection and processing of semen |
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62 | (4) |
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2.2.3. Storage and cryopreservation of semen |
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66 | (4) |
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2.2.4. Insemination procedures |
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70 | (4) |
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2.2.5. Do-it-yourself insemination (DIY-AI) |
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74 | (1) |
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2.2.6. Measuring effectiveness of AI |
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75 | (1) |
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2.3. Semen-sexing Technology |
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75 | (4) |
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2.3.1. Factors influencing the sex ratio |
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76 | (1) |
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2.3.2. Sorting technology |
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77 | (1) |
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2.3.3. Advantages of sex control |
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78 | (1) |
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2.3.4. Problems and prospects in semen sexing |
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79 | (1) |
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2.4. Future Developments in AI Technology |
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79 | (3) |
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2.4.1. Sperm survival at ambient temperature |
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79 | (1) |
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2.4.2. Sperm encapsulation |
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80 | (1) |
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2.4.3. Cryopreservation and freeze-drying of sperm |
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80 | (1) |
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2.4.4. In vitro spermatogenesis |
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81 | (1) |
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82 | (26) |
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3.1. Advantages of Embryo Transfer |
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82 | (7) |
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3.1.1. Cattle and buffaloes |
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82 | (3) |
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85 | (1) |
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86 | (3) |
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89 | (1) |
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3.2. Growth and Development of Embryo Transfer Technology |
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89 | (15) |
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89 | (1) |
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3.2.2. Superovulation techniques |
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90 | (5) |
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3.2.3. Embryo recovery and evaluation |
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95 | (3) |
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3.2.4. In vitro culture and cryopreservation of embryos |
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98 | (4) |
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3.2.5. Donor-recipient synchrony |
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102 | (1) |
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3.2.6. Non-surgical embryo transfer procedures |
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103 | (1) |
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3.2.7. Selection and management of recipients |
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103 | (1) |
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3.3. Practical Applications of Embryo Transfer |
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104 | (3) |
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3.3.1. Embryo transfer and breeding improvement |
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104 | (1) |
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3.3.2. Embryo transfer and gender preselection |
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105 | (1) |
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3.3.3. Genetic preservation of endangered breeds |
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105 | (2) |
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107 | (1) |
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3.4.1. Embryo transfer as a research tool |
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107 | (1) |
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4 In Vitro Embryo Production |
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108 | (32) |
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4.1. Advantages of In Vitro-produced Embryos |
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108 | (6) |
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4.1.1. General considerations |
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108 | (1) |
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4.1.2. Cattle and buffaloes |
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109 | (1) |
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110 | (2) |
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112 | (2) |
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114 | (1) |
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4.2. Growth and Development of Technology |
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114 | (19) |
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114 | (1) |
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4.2.2. Abattoir materials |
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115 | (1) |
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4.2.3. Ovum pick-up (OPU) technique |
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115 | (3) |
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4.2.4. Evaluation and maturation of the oocyte |
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118 | (6) |
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4.2.5. Sperm preparation and in vitro fertilization (IVF) |
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124 | (4) |
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4.2.6. Other fertilization approaches |
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128 | (1) |
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4.2.7. In vitro embryo culture |
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129 | (2) |
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4.2.8. Cryopreservation of embryos and oocytes |
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131 | (1) |
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4.2.9. Evaluating embryo quality |
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132 | (1) |
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4.2.10. Pregnancy rates with fresh and frozen IVP embryos |
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132 | (1) |
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4.3. IVP Embryos in Commerce and in Research |
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133 | (3) |
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4.3.1. Current production statistics |
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133 | (1) |
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4.3.2. In vitro- vs. in vivo-produced embryos |
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133 | (1) |
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4.3.3. Large-offspring syndrome (LOS) |
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134 | (2) |
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136 | (4) |
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4.4.1. Oocytes from pre-antral follicles |
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136 | (1) |
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4.4.2. Gene expression studies |
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137 | (1) |
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4.4.3. Development of microfluidic technology |
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138 | (2) |
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5 Controlling Oestrus and Ovulation |
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140 | (24) |
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5.1. Oestrus and Its Detection |
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140 | (5) |
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5.1.1. Need for accurate oestrus detection |
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141 | (1) |
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5.1.2. Oestrus detection rates |
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142 | (2) |
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144 | (1) |
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5.1.4. Measures of genuine oestrus status |
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145 | (1) |
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5.1.5. Future developments in oestrus detection technology |
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145 | (1) |
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145 | (9) |
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5.2.1. Physiology and endocrinology of the oestrous cycle |
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145 | (4) |
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5.2.2. Monitoring ovarian activity |
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149 | (1) |
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5.2.3. Follicular dynamics |
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149 | (3) |
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5.2.4. Growth and regression of the corpus luteum |
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152 | (2) |
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5.3. Advantages of Oestrus Control |
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154 | (2) |
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5.3.1. Cattle and buffaloes |
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154 | (1) |
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155 | (1) |
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155 | (1) |
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156 | (1) |
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5.4. Growth and Development of Oestrus Control Technology |
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156 | (2) |
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156 | (1) |
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5.4.2. Fertility at the controlled oestrus |
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157 | (1) |
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5.4.3. Accurate control of ovulation |
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158 | (1) |
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5.5. Practical Applications of Technology |
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158 | (6) |
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5.5.1. Control measures currently available |
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159 | (2) |
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5.5.2. Oestrus synchronization and resynchronization |
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161 | (1) |
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5.5.3. Cost-benefit calculations |
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162 | (1) |
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5.5.4. Animal handling considerations |
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163 | (1) |
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6 Control of Post-partum Ovarian Activity |
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164 | (14) |
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6.1. Factors Influencing Post-partum Ovarian Activity |
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164 | (5) |
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6.1.1. Cattle and buffaloes |
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164 | (3) |
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167 | (1) |
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168 | (1) |
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169 | (1) |
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6.2. Development of Control Measures |
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169 | (9) |
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6.2.1. Physiology and endocrinology of the post-partum animal |
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169 | (3) |
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6.2.2. Strategies for inducing resumption of ovarian activity |
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172 | (2) |
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6.2.3. Assessing nutritional status of animals |
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174 | (1) |
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6.3.4. Current treatment protocols |
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174 | (4) |
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7 Control of Seasonal Breeding |
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178 | (17) |
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7.1. Advantages of Control Measures |
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178 | (6) |
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7.1.1. Factors affecting seasonal breeding |
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178 | (1) |
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178 | (3) |
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181 | (1) |
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182 | (1) |
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7.1.5. Buffaloes and camelids |
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183 | (1) |
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184 | (1) |
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7.2. Breeding and Non-breeding Seasons |
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184 | (5) |
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7.2.1. Short- and long-day breeders |
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184 | (1) |
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7.2.2. Physiology and endocrinology of seasonal breeding activity |
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184 | (2) |
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7.2.3. Environmental and genetic factors |
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186 | (3) |
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7.3. Development and Application of Control Measures |
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189 | (6) |
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7.3.1. Practical applications of technology |
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189 | (1) |
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189 | (1) |
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7.3.3. Melatonin treatment |
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190 | (2) |
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7.3.4. Light control in rams and stallions |
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192 | (1) |
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7.3.5. Use of progestogen-PMSG treatments |
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193 | (2) |
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8 Controlling Multiple Births and Litter Size |
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195 | (20) |
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8.1. Advantages of Control Measures |
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196 | (2) |
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196 | (1) |
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197 | (1) |
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198 | (1) |
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8.2. Development of Control Measures |
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198 | (17) |
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198 | (1) |
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8.2.2. Physiology and endocrinology of multiple ovulations |
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199 | (2) |
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8.2.3. Nutritional and environmental factors |
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201 | (1) |
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202 | (2) |
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8.2.5. Use of gonadotrophins |
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204 | (1) |
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8.2.6. Immunization approach |
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205 | (2) |
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207 | (1) |
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8.2.8. Litters and neonatal mortality |
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208 | (5) |
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8.2.9. Animal welfare considerations |
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213 | (2) |
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9 Pregnancy Testing Technology |
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215 | (21) |
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9.1. Advantages of Control Measures |
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215 | (2) |
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9.1.1. Cattle and buffaloes |
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215 | (1) |
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216 | (1) |
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216 | (1) |
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217 | (1) |
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9.2. Factors Affecting Establishment of Pregnancy |
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217 | (7) |
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9.2.1. Physiology and endocrinology of early pregnancy |
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217 | (4) |
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9.2.2. Nutritional and environmental influences |
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221 | (1) |
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9.2.3. Maternal recognition of pregnancy |
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222 | (2) |
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9.3. Pregnancy Testing Methods |
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224 | (12) |
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9.3.1. Palpation per rectum |
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224 | (1) |
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9.3.2. Progesterone and oestrogen assays |
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225 | (3) |
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228 | (1) |
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9.3.4. Predicting litter size |
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229 | (1) |
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9.3.5. Use of ultrasonics |
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230 | (3) |
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9.3.6. Early dipstick tests |
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233 | (1) |
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234 | (2) |
| 10 Controlling Parturition |
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236 | (19) |
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10.1. Advantages of Control Measures |
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236 | (2) |
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236 | (1) |
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236 | (1) |
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237 | (1) |
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10.2. Development of Parturition Control Measures |
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238 | (17) |
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10.2.1. Physiology and endocrinology of late pregnancy and parturition |
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238 | (2) |
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10.2.2. Nutritional influences |
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240 | (1) |
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10.2.3. Factors influencing gestation length |
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241 | (3) |
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10.2.4. Predicting the onset of parturition |
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244 | (1) |
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10.2.5. Physiology and endocrinology of parturition |
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244 | (3) |
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10.2.6. Periparturitional events |
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247 | (2) |
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249 | (4) |
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10.2.8. Delaying parturition |
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253 | (1) |
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10.2.9. Terminating pregnancy |
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253 | (2) |
| 11 Controlling Onset of Puberty |
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255 | (14) |
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11.1. Practical Implications of Early Puberty |
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255 | (4) |
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255 | (2) |
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257 | (1) |
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258 | (1) |
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259 | (1) |
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11.2. Development of Puberty Control Measures |
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259 | (7) |
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11.2.1. Physiology and endocrinology of puberty in farm mammals |
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259 | (3) |
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11.2.2. Genetic and environmental factors |
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262 | (2) |
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11.2.3. Defining pubertal status |
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264 | (1) |
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11.2.4. Puberty and conception |
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265 | (1) |
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11.3. Applications of Technology |
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266 | (3) |
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11.3.1. Management guidelines |
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266 | (1) |
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11.3.2. Hormonal induction of puberty |
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266 | (3) |
| 12 Cloning Technology |
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269 | (13) |
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12.1. Practical Implications of Technology |
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269 | (7) |
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269 | (3) |
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272 | (1) |
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273 | (1) |
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274 | (1) |
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275 | (1) |
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12.2. Developments in Cloning Technology |
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276 | (6) |
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276 | (1) |
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12.2.2. Nuclear transfer procedures |
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277 | (1) |
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12.2.3. Reprogramming the nucleus |
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277 | (2) |
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12.2.4. Gestational and perinatal losses of clones |
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279 | (1) |
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12.2.5. The large-offspring syndrome (LOS) |
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280 | (1) |
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12.2.6. Development of clones after birth |
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280 | (1) |
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12.2.7. Simplification of technology |
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281 | (1) |
| 13 Production of Transgenics |
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282 | (10) |
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13.1. Practical Implications of Technology |
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282 | (6) |
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283 | (2) |
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285 | (1) |
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285 | (1) |
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286 | (1) |
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287 | (1) |
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13.2. Producing Transgenics |
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288 | (4) |
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13.2.1. Improving the technology |
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288 | (1) |
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288 | (1) |
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13.2.3. Transfected cells for nuclear transfer |
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289 | (1) |
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13.2.4. Sperm-mediated DNA transfer |
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289 | (1) |
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13.2.5. Retroviral infection of early embryos |
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290 | (1) |
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13.2.6. Identifying transgenic embryos |
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290 | (1) |
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13.2.7. Future developments |
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291 | (1) |
| 14 Suppressing Reproductive Activity |
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292 | (6) |
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14.1. Advantages of Technology |
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292 | (2) |
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293 | (1) |
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293 | (1) |
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294 | (1) |
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294 | (1) |
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294 | (1) |
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14.2. Development and Application of Technology |
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294 | (4) |
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14.2.1. Hormonal approach |
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294 | (2) |
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14.2.2. Immunological approach |
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296 | (1) |
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14.2.3. Wild animal populations |
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297 | (1) |
| Bibliography |
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298 | (19) |
| Appendix A |
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317 | (2) |
| Appendix B |
|
319 | (1) |
| Index |
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320 | |