Edited by the world's foremost experts, this thoroughly updated, comprehensive, state-of-the-art reference, details the latest research developments and challenges in the biophysical and single molecule approaches to RNA nanotechnology.
Interest in RNA nanotechnology has increased in recent years as recognition of its potential for applications in nanomedicine has grown. Edited by the world's foremost experts in nanomedicine, this comprehensive, state-of-the-art reference details the latest research developments and challenges in the biophysical and single molecule approaches in RNA nanotechnology. In addition, the text also provides in-depth discussions of RNA structure for nanoparticle construction, RNA computation and modeling, single molecule imaging of RNA, RNA nanoparticle assembly, RNA nanoparticles in therapeutics, immunorecognition of RNA nanomaterials, RNA chemistry for nanoparticle synthesis, and conjugation and labeling.
- Presents the latest research and discoveries in RNA nanotechnology
- Features contributions from world-class experts in the field
- Covers RNA nanoparticles in therapeutics
- Describes self-assembled RNA nanoparticles
Arvustused
"This comprehensive, state-of-the-art reference book, edited by experts in the field of nanomedicine, describes the latest research development and challenges in the biophysical and single-molecule approaches in RNA nanotechnology. The book provides interesting coverage on RNA nanoparticles in therapeutics and details self-assembled RNA nanoparticles."
-Omer Iqbal, MD, FACC, FESC, Doody Review
Part I: Concepts and Definitions in RNA Nanotechnology.
1. RNA Structure
and Folding.
2. RNA Regulation and Function in Nature.
3. Principles and
Fundamentals of RNA Nanotechnology.
4. Computation and Folding Predictions.
5. Enzymatic Synthesis and Modification of RNA Nanoparticles.
6. Synthetic
and Enzymatic Methods for RNA Labeling and Modifications.
7. Methods and
Assembly of RNA Nanotechnology.
8. Purification, Characterization, and
Structure Determination of RNA Nanoparticles.
9. SELEX.
10. Uniqueness,
Advantages, Challenges, Solutions, and Perspectives in Therapeutics Applying
RNA Nanotechnology. Part II: Design, Synthesis, and Characterization Methods
in RNA Nanotechnology.
11. The Natural Versatility of RNA.
12. Nucleic Acids
as a building material in nanotechnology.
13. Multiple functionalities for
RNA Nanoparticles.
14. From Computational RNA Structure Predication to the
Design of Biologically Active RNA-based Nanostructures.
15. Application of
RNA tertiary structure prediction tool iFoldRNA in RNA nanotechnology.
16.
HyperFold: A Web Server for Predicating Nucleic Acid Complexes.
17. RNA
Switches: Towards Conditional Dynamic RNA-based Constructs for Therapeutics
and Bioassays.
18. RNA Multiway Junction Motifs as Lego for Construction of
Multifunctional RNA Nanoparticles.
19. Fabrication Methods for RNA
Nanoparticle Assembly Based on Bacteriophage Phi29 PRNA Structural Features.
20. Purification of RNA, modified oligos and RNA nanoparticles.
21.
Physicochemical Characterization of Nucleic Acid Nanoparticles.
22. Light
Scattering Techniques for Characterization of NANPs and Their Formulations.
23. Electron Microscopy of Nucleic Acid Nanoparticles.
24. A Single FRET
Approach for Investigating the Binding Mechanism of Anti-viral Aptamers.
25.
Entropy and Enthalpy in RNA Nanoparticle Assembly and Thermodynamic Stability
for Medical Application Using RNA Nanotechnology. Part III: Immunorecognition
of RNA Nanoparticles.
26. Immunorecognition of nucleic acid nanoparticles.
27. Viral noncoding RNAs in modulating cellular defense and their potential
for RNA nanotechnology.
28. RIG-I as a therapeutic target for Nucleic Acid
Nanoparticles (NANPs).
29. Driving Dynamic Functions with Programmable RNA
Nanostructures.
30. Use of human peripheral blood mononuclear cells to define
immunological properties of nucleic acid nanoparticles. Part IV: Delivery of
Functional RNA Nanoparticles.
31. The emerging field of RNA Nanotechnology.
32. Thermodynamically stable RNA three-way junction for constructing
multifunctional nanoparticles for delivery of therapeutics.
33. RNAi
Nanotherapeutics for Localized Cancer Therapy.
34. Delivery of RNA
nanoparticles.
35. Recommendations for Planning in vivo Studies for RNAi
Therapeutics.
36. Mesoporous silica nanoparticles for efficient siRNA
delivery.
37. Method of Large-scale Exosome Purification and Its Use for
Pharmaceutical Applications.
38. Engineered extracellular vesicle based
therapeutics for liver cancer.
39. Extracellular Vesicles: An innovative
approach to engineering nucleic acid delivery.
40. Extracellular Vesicles
(EVs): Naturally Occurring Vehicles for RNA Nanotherapeutics.
41. Harnessing
exosomes and bioinspired exosome-like nanoparticles for siRNA delivery. Part
V: Application and Exploitation in RNA Nanotechnology.
42. RNA structural
modeling for therapeutic applications.
43. RNA micelles for therapeutics
delivery and cancer therapy.
44. Bacteriophage RNA Leading the Way in RNA
Nanotechnology for Targeted Cancer Therapy.
45. Current State in development
of RNAi self-assembled nanostructures.
46. RNA-Based Devices for Diagnostic
and Biosensing.
47. MicroRNAs: Biology and Role in RNA Nanotechnology.
48.
Conjugation of RNA aptamer to RNA nanoparticles for targeted drug delivery.
49. MicroRNAs in human cancers and therapeutic applications.
50. Tuning the
Size, Shape and Structure of RNA Nanoparticles for Favorable Cancer Targeting
and Immunostimulation.
51. Application of RNA nanotechnology in Exosome for
gene therapy.
52. Application of RNA Aptamers in Nanotechnology and
Therapeutics.
Peixuan Guo is the Director of the NIH Nanomedicine Development Center at the University of Cincinnati, Ohio and the Director of the NIH/NCI Cancer Nanotechnology Platform Partnership Program: RNA Nanotechnology for Cancer Therapy at Purdue University, Indiana. He is also a Fellow of the National Academy of Inventors.
Kirill Afonin is the Professor of Chemistry at University of North Carolina at Charlotte. He currently serves as the Founding Council Member and Vice President of International Society of RNA Nanotechnology and Nanomedicine (isrnn.org) and is recipient of several prestigious awards among which is Maximizing Investigators' Research Award (MIRA R35) from NIH.
