Introduction. Genome Data Mining Through Modern Sequencing Technologies. Comparison of Next-Generation Sequencing Systems. Why Assembling Plant Genome Sequences Is So Challenging. Genome Walking by Next Generation Sequencing Approaches. Molecular Tools for Exploring Polyploid Genomes in Plants. Wheat Genomics: Present Status and Future Prospects. Development in Rice Genome Research Based on Accurate Genome Sequence. Genomic Databases for Crop Improvement. Going Beyond DNA Variations to Understand Environmental Adaptations. Uncovering the Complexity of Transcriptomes with RNA-Seq. Efficient Experimental Design and Analysis Strategies for the Detection of Differential Expression Using RNA-Sequencing. Analyzing the microRNA Transcriptome in Plants Using Deep Sequencing Data. Maize (Zea mays L.) Genome Diversity as Revealed by RNA-Sequencing. Molecular Mechanisms of Epigenetic Variation in Plants. Metagenomics: A Guide from Sampling to Data Analysis. Single Nucleotide Polymorphism is the Ideal Marker. NP Markers and Their Impact on Plant Breeding. Genotyping-by-Sequencing in Plants. Index.