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Skin Stem Cells: Methods and Protocols Third Edition 2024 [Pehme köide]

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  • Formaat: Paperback / softback, 266 pages, kõrgus x laius: 254x178 mm, 45 Illustrations, color; 8 Illustrations, black and white
  • Sari: Methods in Molecular Biology
  • Ilmumisaeg: 21-Jul-2025
  • Kirjastus: Humana
  • ISBN-10: 1071640933
  • ISBN-13: 9781071640937
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  • Formaat: Paperback / softback, 266 pages, kõrgus x laius: 254x178 mm, 45 Illustrations, color; 8 Illustrations, black and white
  • Sari: Methods in Molecular Biology
  • Ilmumisaeg: 21-Jul-2025
  • Kirjastus: Humana
  • ISBN-10: 1071640933
  • ISBN-13: 9781071640937
Teised raamatud teemal:
This updated edition explores techniques in identifying and characterizing various skin stem cells and generating skin-like structures in vitro. The goal of the book is to present exciting advances in skin biology in an effort to provide a better understanding of skin and, eventually, to allow us to regenerate skin with its original form and function. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.





 





Authoritative and up-to-date, Skin Stem Cells: Methods and Protocols, Third Edition serves as a valuable assemblage of protocols for researchers already working in the field and for those who now wish to newly embark on studies of skin stem cells.
Isolation, Characterization, and Utilization of Human Skin Basal and
Supra-Basal Epidermal Stem Cells.- Isolation of Human Progenitor Epidermal
Cells on Collagen Type IV and Analysis of Their Markers with Flow Cytometry
and PCR Methods.- Approaches to Study Wound-Induced Hair Neogenesis (WIHN).-
Characterization of the Newborn Epidermis and Adult Hair Follicles Using
Whole-Mount Immunofluorescent Staining of Mouse Dorsal Skin.- Preparation and
Use of shRNA for Knocking Down Specific Genes.- Efficient Generation of
CRISPR/Cas9-Mediated Knockout Human Primary Keratinocytes by
Electroporation.- Application of CRISPR/Cas9 Pooled Screening for a
Non-Immediate Readout Model.- Contribution of Artificial Intelligence to the
Identification of Protein-Protein Interactions: A Case Study on PAR-3 and Its
Partner Adapter Molecule Crk.- Site-Directed Mutagenesis to Mutate Multiple
Residues in a Single Reaction.- Analysis of Proliferation, Apoptosis, and
Motility in Mouse Embryonic Melanocytic Precursor Cells.- Protocol for
Testing Human Melanoma Exosomes in Shifting Healthy Phenotype of Human Dermal
Cells.- Incorporating NK Cells in a Three-Dimensional Organotypic Culture
System for Human Skin Stem Cells: Modeling Skin Diseases and Immune Cell
Interplay.- Standardized GMP-Compliant Scalable 3D-Bioprocessing of Epidermal
Stem Cells for Diabetic Foot Ulcers.- Differentiation and Validation of Human
Induced Pluripotent Stem Cells into a Keratinocyte, as Therapeutic Option in
Vitiligo.- GMP-Based Isolation of Full-Term Human Placenta-Derived NK Cells
for CAR-NK Cell Therapy in Malignant Melanoma.- Isolation and Phenotypic
Characterization of Tumor-Infiltrating NK Cells in Skin Carcinoma.-
Preparation and Validation of Zebrafish Psoriasis Model to Investigate the
Therapeutic Effects of Stem Cells.- Flow Cytometric and Immunohistochemical
Follow-Up of Spermatogonial Lineage Commitment.- Development of CAR-NK Cells
Targeting cSCC-Specific Antigens for Precision Immunotherapy.