This updated collection features protocols that reflect the continued expansion of stem cell niche research. These specialized microenvironments that regulate stem cell function have continued to inspire tremendous interest as a subject of study in the years since the publication of the second edition. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Stem Cell Niche: Methods and Protocols, Third Edition serves as an ideal guide for both experts and novices in the stem cell field.
Human Skeletal Muscle Niche Formation and Analysis with Spatial RNA
Sequencing.- Three-Dimensional Analysis of Skeletal Muscle Stem Cell Niche
Following Tissue Clearing.- Efficient Isolation and Ex Vivo Differentiation
of Murine Satellite Cells from Healthy and Dystrophic Muscle.- Isolation of
Mouse Bone Marrow Niche Cells for Single Cell Sequencing.- Microwells as
Minimalistic Niches to Study Heterotypic Interactions of Stromal and
Hematopoietic Stem Cells.- Detection of Cellular Senescence on Murine Muscle
Tissue Sections by Senescence-Associated -Galactosidase Staining.-
Light-Sheet Microscopy Enables Three-Dimensional Fluorescence Imaging and
Live Imaging of Satellite Cells on Skeletal Muscle Fibers.- Stem Cell Niche:
iPSC-Based Assembloids for Modeling Human Hematopoiesis.- Oligopaint FISH in
Drosophila Testes.- Evaluation of Adult Mouse Brain Neurogenic Niche Behavior
by Brain Slices In Vitro Culture Method.- Isolation of Murine Neonatal and
Adult Osteomacs to Examine Their Role in the Hematopoietic Niche.- Telocytes:
Detection, Visualization, Tissue Dissociation, and Tamoxifen-Induction of
Transgenic Mice.- Co-Colonization and Co-Culture of Lung Alveoli Epithelial
Stem Cells and Their Endothelial Niche Cells.- Simplified Quantification of
Progenitor Zone Size, an Indicator of Germ Stem Cell Niche Activity, in the
Nematode Caenorhabditis elegans.- Measurement of Myonuclear Accretion In
Vitro and In Vivo.- A Simple Method to Dissect, Orientate, and Visualize the
Murine Limbal Stem Cell Niche with Cornea and Conjunctiva Attached.-
Regeneration of Volumetric Muscle Loss Using MSCs Encapsulated in PRP-Derived
Fibrin Microbeads.- Standardizing Chondrocyte Isolation and Articular
Cartilage Decellularization: A Versatile Bioink for Tissue Engineering
Applications.- Electrospinning Approach to Preparing Nanostructured Scaffolds
for Stem Cell Seeding and/or Implantation in Neurotrauma.- Development of a
Three-Dimensional Multicellular Model of Human Neuroblastoma Using Matrigel
as an Extracellular Matrix Analogue.- Principles of Indirect Co-Culture
Method Using Transwell.- Upstream Process Protocol for MSCs Isolated from
Different Human-Based Tissue Origins.- Isolation and Culturing of Newborn Rat
Nasal Chondrocytes.
