Muutke küpsiste eelistusi

Structure-Function Toolbox for Membrane Transporter and Channels, Volume 594 [Kõva köide]

Volume editor (Department of Structural Biology, Max Planck Institute of Biophysics, Germany)
  • Formaat: Hardback, 302 pages, kõrgus x laius: 229x152 mm, kaal: 610 g
  • Sari: Methods in Enzymology
  • Ilmumisaeg: 04-Aug-2017
  • Kirjastus: Academic Press Inc
  • ISBN-10: 0128123532
  • ISBN-13: 9780128123539
Teised raamatud teemal:
  • Kõva köide
  • Hind: 149,06 €*
  • * hind on lõplik, st. muud allahindlused enam ei rakendu
  • Tavahind: 198,75 €
  • Säästad 25%
  • Raamatu kohalejõudmiseks kirjastusest kulub orienteeruvalt 3-4 nädalat
  • Kogus:
  • Lisa ostukorvi
  • Tasuta tarne
  • Tellimisaeg 2-4 nädalat
  • Lisa soovinimekirja
Structure-Function Toolbox for Membrane Transporter and Channels, Volume 594Suurem pilt
  • Formaat: Hardback, 302 pages, kõrgus x laius: 229x152 mm, kaal: 610 g
  • Sari: Methods in Enzymology
  • Ilmumisaeg: 04-Aug-2017
  • Kirjastus: Academic Press Inc
  • ISBN-10: 0128123532
  • ISBN-13: 9780128123539
Teised raamatud teemal:
Teised raamatud sellest sooduspakkumisest: Kevadine sooduspakkumine - kuni 31. maini üle miljoni raamatu kuni 25% soodsamalt
Püsilink: https://www.kriso.ee/db/9780128123539.html
Märksõnad:
A Structure-Function Toolbox for Membrane Transporter and Channels, Volume 594, a new release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. New chapters in the updated serial include Cryo-EM on membrane proteins embedded in nanodics, Solid-Supported membrane-based electrophysiology on membrane transporters and channels, Saposin-lipoprotein scaffolds for structure determination of membrane transporters and channels, Single-molecule FRET on transporters, Dynamics of channels and transporters investigated by NMR, Structure-function studies on channels and transporters, and a section on MemStar, a new GFP-based expression and purification system for transporters and channels.

Arvustused

Praise for the Series: "Should be on the shelves of all libraries in the world as a whole collection." --Chemistry in Industry "The work most often consulted in the lab." --Enzymologia "The Methods in Enzymology series represents the gold-standard." --Neuroscience

Muu info

Continues the legacy of this premier serial with quality chapters authored by leaders in the field of enzymology
Contributors ix
Preface xi
1 Lipid Nanodiscs as a Tool for High-Resolution Structure Determination of Membrane Proteins by Single-Particle Cryo-EM
1(30)
Rouslan G. Efremov
Christos Gatsogiannis
Stefan Raunser
1 Introduction
2(7)
2 General Considerations
9(5)
3 Single-Particle Cryo-EM of the Ryanodine Receptor RyRI Reconstituted Into Lipid Nanodiscs
14(6)
4 EM Analysis of the TcdA1 Pore Complex Embedded in Lipid Nanodiscs
20(3)
5 Conclusions and Perspectives
23(8)
Acknowledgments
24(1)
References
24(7)
2 SSM-Based Electrophysiology for Transporter Research
31(54)
Andre Bazzone
Maria Barthmes
Klaus Fendler
1 Background
32(6)
2 Technical Implications
38(15)
3 Description of the Workflow
53(11)
4 Assay Development
64(9)
5 Data Interpretation and Analysis
73(12)
References
80(5)
3 Saposin-Lipoprotein Scaffolds for Structure Determination of Membrane Transporters
85(16)
Joseph A. Lyons
Andreas Boggild
Poul Nissen
Jens Frauenfeld
1 Introduction
86(1)
2 Membrane Protein Reconstitution
87(7)
3 EM of Salipro Particles
94(3)
4 Summary and Conclusion
97(4)
Acknowledgments
98(1)
References
98(3)
4 Single-Molecule Fluorescence Studies of Membrane Transporters Using Total Internal Reflection Microscopy
101(22)
Joris M.H. Goudsmits
Antoine M. van Oijen
Dirk J. Slotboom
1 Introduction
102(3)
2 Preparing Membrane Proteins and Lipid Membranes
105(5)
3 Single-Molecule Fluorescence Microscopy
110(5)
4 Data Analysis
115(8)
Acknowledgments
118(1)
References
118(5)
5 The MEMbrane Protein Single ShoT Amplification Recipe: MemStar
123(16)
Abdul Aziz-Qureshi
Pascal F. Meier
Chiara Lee
David Drew
1 Introduction
124(4)
2 Materials
128(3)
3 Procedure
131(5)
4 Summary and Conclusion
136(3)
Acknowledgments
137(1)
References
137(2)
6 Efficient Screening and Optimization of Membrane Protein Production in Escherichia coli
139(26)
Jacopo Marino
Katharina Holzhuter
Benedikt Kuhn
Eric R. Geertsma
1 Introduction
140(6)
2 Preparation of Expression Vectors and Cloning
146(8)
3 Analysis of Membrane Protein Production Using GFP
154(5)
4 Summary and Conclusion
159(6)
Acknowledgments
160(1)
References
160(5)
7 Pinning Down the Mechanism of Transport: Probing the Structure and Function of Transporters Using Cysteine Cross-Linking and Site-Specific Labeling
165(38)
Christopher Mulligan
Joseph A. Mindell
1 Introduction
167(2)
2 The Unique and Useful Chemistry of Cysteine
169(2)
3 The Many Uses of Cysteine
171(6)
4 Cysteine-Reactive Reagents Used for Cross-Linking and Labeling
177(4)
5 Methods of Detecting Cross-Link Formation
181(9)
6 Strategies Used to Decide the Position of Cysteine Residues
190(3)
7 Detailed Protocol for Cross-Linking a Transporter
193(10)
References
196(7)
8 Ion Channel Conformation and Oligomerization Assessment by Site-Directed Spin Labeling and Pulsed-EPR
203(40)
Christos Pliotas
1 Introduction
204(2)
2 Designing the Pulsed-EPR Experiment
206(8)
3 Sample Preparation for EPR Experiments
214(7)
4 The EPR Methodology Toolbox
221(22)
Acknowledgments
238(1)
References
238(5)
9 Strategy for the Thermostabilization of an Agonist-Bound GPCR Coupled to a G Protein
243(22)
Annette Strege
Byron Carpenter
Patricia C. Edwards
Christopher G. Tate
1 Introduction
244(2)
2 A Thermostability Assay for Measuring the Stability of GPCR-G Protein Complexes
246(11)
3 Summary and Conclusion
257(8)
Acknowledgments
262(1)
References
262(3)
Author Index 265(16)
Subject Index 281
Prof. Dr. Christine Ziegler works at the Department of Structural Biology, Max Planck Institute of Biophysics, Germany