This volume covers methods to study protein modification by the Small Ubiquitin-like Modifier (SUMO) family of proteins, post-translational modifiers with essential roles in physiology and pathology. The book explores methods to detect SUMOylated proteins, identify SUMO modified sites, characterize the deposition and removal of these modifications, and explore how SUMO modifies protein-protein interactions both in vitro and in cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.
Authoritative and practical, SUMO: Methods and Protocols serves as an ideal guide for researchers seeking the most relevant and cutting-edge technologies to dissect protein SUMOylation.
