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Super-Resolution Microscopy: Methods and Protocols [Kõva köide]

  • Formaat: Hardback, 330 pages, kõrgus x laius: 254x178 mm, 71 Illustrations, color; 6 Illustrations, black and white
  • Sari: Methods in Molecular Biology
  • Ilmumisaeg: 01-May-2026
  • Kirjastus: Humana
  • ISBN-10: 1071652672
  • ISBN-13: 9781071652671
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  • Formaat: Hardback, 330 pages, kõrgus x laius: 254x178 mm, 71 Illustrations, color; 6 Illustrations, black and white
  • Sari: Methods in Molecular Biology
  • Ilmumisaeg: 01-May-2026
  • Kirjastus: Humana
  • ISBN-10: 1071652672
  • ISBN-13: 9781071652671
This volume provides comprehensive methods used to perform super-resolution microscopy and its analysis. Each chapter is written by a leading expert in the field, covering fundamentals of super-resolution microscopy, single-molecule localization microscopy (SMLM), stimulated emission depletion (STED) microscopy, expansion microscopy, and others. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and thorough, Super-Resolution Microscopy: Methods and Protocols provides well-established protocols used by both academics and industrials.
Structured Illumination Microscopy.- Multicolor super-resolution
fluorescence imaging of cells.- Super-resolution spinning-disk confocal
microscopy using optical photon reassignment.- Improving STED-based
super-resolution-imaging with fluorescence lifetimes.- Single Molecule
Localization Microscopy with Fixed Photoactivatable Fluorescent Proteins and
Direct Stochastic Optical Reconstruction Microscopy.- Single particle
tracking Photo-Activated Localization Microscopy.- Single Molecule
Localisation Microscopy in the nucleus with self-labelling tags: Halo, SNAP
and CLIP tags.- Single Molecule Localisation Microscopy in vivo at the
Drosophila neuromuscular junction.- Tracking synaptic vesicles in live
neurons using single-molecule super-resolution microscopy.- Single molecule
imaging of endogenous proteins.- Shadow imaging of brain microanatomy in live
and fixed tissue.- NanoPlex: A universal strategy to multiplex
immunostainings for fluorescence microscopy using secondary nanobodies.-
Spatiotemporal analysis of super-resolved live cell molecular trajectory data
using nanoscale spatiotemporal indexing clustering.- Case study: Illuminating
the nanoscale world of microbiology.