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Super-Resolution Microscopy: Methods and Protocols Softcover reprint of the original 1st ed. 2017 [Pehme köide]

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  • Formaat: Paperback / softback, 262 pages, kõrgus x laius: 254x178 mm, kaal: 5235 g, 37 Illustrations, color; 11 Illustrations, black and white; XI, 262 p. 48 illus., 37 illus. in color., 1 Paperback / softback
  • Sari: Methods in Molecular Biology 1663
  • Ilmumisaeg: 18-May-2018
  • Kirjastus: Humana Press Inc.
  • ISBN-10: 1493984357
  • ISBN-13: 9781493984350
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  • Formaat: Paperback / softback, 262 pages, kõrgus x laius: 254x178 mm, kaal: 5235 g, 37 Illustrations, color; 11 Illustrations, black and white; XI, 262 p. 48 illus., 37 illus. in color., 1 Paperback / softback
  • Sari: Methods in Molecular Biology 1663
  • Ilmumisaeg: 18-May-2018
  • Kirjastus: Humana Press Inc.
  • ISBN-10: 1493984357
  • ISBN-13: 9781493984350
Teised raamatud teemal:

This volume presents current advanced technologies and methods used in super-resolution microscopy. The chapters in this book cover a wide range of topics such as introducing super-resolution microscopy into a core facility; two-photon STED microscopy for nanoscale imaging of neural morphology in vivo; correlative SIM-STORM microscopy; two-color single-molecule tracking in live cells; and correlative single molecule localization microscopy and confocal microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Super-Resolution Microscopy: Methods and Protocols is a valuable resource for both established and novel researchers and users in this field.

Arvustused

The aim is to provide a current overview of the advanced technologies and methods in superresolution microscopy. The book is written for novices andexperienced investigators. It will be of much help for graduate students and postdoctoral students interested in super-resolution microscopy. The author is a credible authority in the field of super-resolution microscopy. This book covers advanced, cutting-edge techniques related to super-resolution microscopy. The intended audience will find it of immense help. (Omer Iqbal, Doody's Book Reviews, February, 2018)

Super-Resolution Microscopy Techniques and their Potential for
Applications in Radiation Biophysics.- Managing the Introduction of
Super-Resolution Microscopy into a Core Facility.- Live-Cell STED Imaging
with the HyPer2 Biosensor.- Diffraction-Unlimited Fluorescence Imaging with
an EasySTED Retrofitted Confocal Microscope.- Two-Photon STED Microscopy for
Nanoscale Imaging of Neural Morphology In Vivo.- STED Imaging of Golgi
Dynamics with Cer-SiR: A Two-Component, Photostable, High-Density Lipid Probe
for Live Cells.- Four Channel Super-Resolution Imaging by 3-D Structured
Illumination.- Correlative SIM-STORM Microscopy.- Correlative
Super-Resolution Fluorescence Imaging and Atomic Force Microscopy for the
Characterization of Biological Samples.- Quantitative Single-Molecule
Localization Microscopy (qSMLM) of Membrane Proteins Based on Kinetic
Analysis of Fluorophore Blinking Cycles.- Two-Color Single-Molecule Tracking
in LiveCells.- Fully Automated Targeted Confocal and Single Molecule
Localization Microscopy.- Brain Slice Staining and Preparation for
Three-Dimensional Super-Resolution Microscopy.- Correlative In-Resin
Super-Resolution Fluorescence and Electron Microscopy of Cultured
Cells.- Synthesis of Janelia Fluor HaloTag and SNAP-Tag Ligands and their use
in Cellular Imaging Experiments.- Measuring Nanometer Distances between
Fluorescent Labels Step-by-Step.- Correlative Single Molecule Localization
Microscopy and Confocal Microscopy.- Correlative Fluorescence
Super-Resolution Localization Microscopy and Platinum Replica EM on Unroofed
Cells.- In Situ Super-Resolution Imaging of Genomic DNA with OligoSTORM and
OligoDNA-PAINT.- Super-Resolution High Content Screening and Analysis.