| Preface |
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| Contributors |
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1 Trypanosoma cruzi Genome Assemblies: Challenges and Milestones of Assembling a Highly Repetitive and Complex Genome |
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1 | (22) |
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2 Mining Trypanosoma cruzi Genome Sequences for Antigen Discovery and Vaccine Development |
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23 | (12) |
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3 Transcriptome Studies in Trypanosoma cruzi Using RNA-seq |
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35 | (12) |
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4 An Improved Approach to Trypanosoma cruzi Molecular Genotyping by Next-Generation Sequencing of the Mini-exon Gene |
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47 | (14) |
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5 Genome Editing by CRISPR/Cas9 in Trypanosoma cruzi |
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61 | (16) |
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6 Sample Preparation of Trypanosoma cruzi Surface, Extracellular, and Nuclear Subproteomes |
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77 | (12) |
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Carlos Andre Ornelas Ricart |
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Rayner Myr Lauterjung Queiroz |
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Agenor De Castro Moreira Dos Santos Junior |
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7 Isolation and Characterization of Extracellular Vesicles Derived from Trypanosoma cruzi |
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89 | (16) |
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8 Production of Recombinant Trypanosoma cruzi Antigens in Leishmania tarentolae |
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105 | (14) |
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9 Homologous Expression of Glycosylphosphatidylinositol-Anchored Glycoproteins in Trypanosoma cruzi |
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119 | (16) |
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Maria De Los Milagros Cdmara |
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10 Metabolic Labeling of Surface Neo-sialylglyconjugates Catalyzed by Trypanosoma cruzi frawj-Sialidase |
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135 | (12) |
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Maria De Los Milagros Cdmara |
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11 Exploiting Genetically Modified Dual-Reporter Strains to Monitor Experimental Trypanosoma cruzi Infections and Host-Parasite Interactions |
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147 | (18) |
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12 Transmigration of Trypanosoma cruzi Trypomastigotes through 3D Spheroids Mimicking Host Tissues |
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165 | (14) |
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Matias Exequiel Rodriguez |
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13 In Situ Detection of Dormant Trypanosoma cruzi Amastigotes Using Bioluminescent-Fluorescent Reporters |
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179 | (8) |
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14 Multi-Parametric Evaluation of Trypanosoma cruzi Infection Outcome in Animal Models |
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187 | (16) |
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15 MicroRNA Transcriptome Profiling in Heart of Trypanosoma cruzi-lnkctcd Mice |
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203 | (12) |
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Ludmila Rodrigues Pinto Ferreira |
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16 Assessing Parasite Load in Chagas Disease Patients by Quantitative Multiplex Real-Time PCR |
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215 | (12) |
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17 Genotyping of Trypanosoma cruzi from Clinical Samples by Multilocus Conventional PCR |
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227 | (12) |
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18 Diagnostic Applicability of Neutralizing Antibodies to Trypanosoma cruzi Traw-sialidase |
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239 | (8) |
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19 Analytics of Functional Autoantibodies in Patients with Chagas Disease |
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247 | (16) |
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20 Apolipoprotein Al and Fibronectin Fragments as Markers of Cure for the Chagas Disease |
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263 | (12) |
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21 Host-Derived Molecules as Novel Chagas Disease Biomarkers: Hypercoagulability Markers in Plasma |
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275 | (12) |
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22 Purification of Glycosylphosphatidylinositol-Anchored Mucins from Trypanosoma cruzi Trypomastigotes and Synthesis of ct-Gal-Containing Neoglycoproteins: Application as Biomarkers for Reliable Diagnosis and Early Assessment of Chemotherapeutic Outcomes of Chagas Disease |
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287 | (22) |
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Belkisyole Alarcbn De Noya |
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23 Cytometric Bead Array (CBA) for Measuring Cytokine Levels in Chagas Disease Patients |
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309 | (6) |
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24 Generation of T. cruzi-Specific Primary CD4+ T Cell Lines from Peripheral Blood Mononuclear Cells Isolated from Chagas Disease Patients |
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315 | (24) |
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25 Trypanosoma cruzi-Specific T-Cell Responses to Monitor Treatment Efficacy in Chronic Chagas Disease |
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339 | (10) |
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Melisa Daiana Castro Eiro |
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26 CD8+ T Cell Response to Trypanosoma cruzi Antigens during Chronic Chagas Disease |
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349 | (14) |
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27 Fluorescence Proteomic Technology to Analyze Peripheral Blood Mononuclear Cells in Chronic Chagas Disease |
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363 | (18) |
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28 Isolation and Phenotypic Characterization of Inflammatory Cells from Clinical Samples: Purification of Macrophages from Trypanosoma cruzi-Infected Hearts |
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381 | (16) |
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| Index |
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