T. cruzi Infection: Methods and Protocols Second Edition 2026 [Kõva köide]

Computer-Aided Discovery of Drugs Against Chagas Disease.- Chromatin
Interaction Maps of Trypanosoma cruzi.- GCanner, a Genome-Wide GC Composition
Tool for the Unbiased Assessment of Trypanosoma cruzi Genomic Compartments.-
An Algorithm for Annotation of Trypanosoma cruzi MASP Sequences Employing
Hidden Markov Models.- Cloning-Free Genome Editing by CRISPR/T7RNAP/Cas9 in
Trypanosoma cruzi.- Typification of Trypanosomatids Using Amplicon-Based
Sequencing.- Deep Amplicon Sequencing of Minicircle Hypervariable Regions
(mHVRs) for High-Resolution and Sensitive Trypanosoma cruzi Genotyping.-
Identification of Trypanosoma cruzi Discrete Typing Units by Multiplex
Real-Time PCR.- Real-Time PCR Assays for Quantification of Trypanosoma cruzi
in Food Samples: A Tool for Surveillance of Oral Chagas Disease Outbreaks.-
Ultra-Rapid DNA Extraction Coupled with Loop-Mediated Isothermal
Amplification for Point-of-Care Detection of Trypanosoma cruzi Infection in
Heparinized Blood and Dried Blood Spots.- Quantitative Real-Time PCR for
Trypanosoma cruzi Infection Diagnosis and Treatment Response Monitoring of
Patients with Chagas Disease.- Standardization of Serodiagnostic Methods for
Trypanosoma cruzi.- Software and Tools to Analyze High-Density Peptide Array
Data for the Chagas Antigen and Epitope Atlas.- Conjugation of Saccharidic
Synthetic Antigens with Carrier Proteins by the Squaric Acid Method:
Application as Biomarkers for Treatment Evaluation in Chagas Disease.-
Identifying Trypanosoma cruzi Proteins Targeted by Chagas Disease Patient
Antibodies Using Yeast Surface Display.- Profiling T-Cell Receptor and
Immunoglobulin Repertoires through RNA-Sequencing to Understand Host Response
to Trypanosoma cruzi Infection.- The Use of B-Cell Tetramers for the Study of
Trypanosoma cruzi-Specific B-Cell Responses.- ELISPOT-Based Measurement of
Antibody Secreting Cells and Memory B-Cells Specific to Trypanosoma cruzi in
Chronically Infected Subjects.- Host-Derived Molecules as Novel Chagas
Disease Biomarkers: Hypercoagulability Markers and Extracellular Vesicles.-
Advanced Identification of Trypanosoma cruzi-Specific CD4+ and CD8+ T-Cells
in Chronic Chagas Disease Patients Using Activation-Induced Marker (AIM)
Assay.- Assessing the Metabolic Profile of CD4+ and CD8+ T-Lymphocytes in
Chronic Chagas Disease Patients Using SCENITH.- A Single Cell Methodology for
Energy Metabolism Analysis in Parasitic Protozoa.- Determining Histone
Post-Translational Modifications along the Cell Cycle in Trypanosoma cruzi by
Flow Cytometry.- Ultrastructure Expansion Microscopy for the Analysis of
Trypanosoma cruzi Infection.- An In Vitro 3D Collagen-I Model for Studying
Trypanosoma cruzi Trypomastigote Motility.- A Rapid Method for Purifying
Intact Trypanosoma cruzi Amastigotes from Host Cells, In Vitro and In Vivo,
for Downstream Omic Analysis.- Insights into the Procedures and
Interpretation of Autophagy in Trypanosoma cruzi.- Animal Models for Studying
Trypanosoma cruzi Congenital Transmission and Associated Pathogenesis:
Special Emphasis on Guinea Pig Model.