Muutke küpsiste eelistusi

Techniques for Work with Plant and Soil Nematodes [Kõva köide]

Edited by (University of Hertfordshire, UK), Edited by (CABI, UK), Edited by (California Department of Food and Agriculture, USA)
  • Formaat: Hardback, 312 pages, kõrgus x laius x paksus: 246x189x20 mm, kaal: 1015 g
  • Ilmumisaeg: 18-Dec-2020
  • Kirjastus: CABI Publishing
  • ISBN-10: 1786391759
  • ISBN-13: 9781786391759
Teised raamatud teemal:
  • Formaat: Hardback, 312 pages, kõrgus x laius x paksus: 246x189x20 mm, kaal: 1015 g
  • Ilmumisaeg: 18-Dec-2020
  • Kirjastus: CABI Publishing
  • ISBN-10: 1786391759
  • ISBN-13: 9781786391759
Teised raamatud teemal:
This up-to-date, comprehensive book covers the practicalities of working with and studying soil and plant nematodes. Written by an international team of experts, it includes traditional and new methodologies, as well as sections on behavioural and physiological assays, and cytogenetic, biochemical and molecular biology techniques.

Techniques for Work with Plant and Soil Nematodes is an up-to-date, comprehensive book covering the practicalities of working with and studying soil and plant nematodes. Written by an international team of experts, this book is highly illustrated and provides thorough coverage of methods while allowing for relevant information to be located quickly. It includes the fundamental traditional techniques and new methodologies, covering: sampling; extraction; estimating numbers; handling, fixing, staining, mounting; culturing techniques; figure preparation, measurement and image processing; electron microscopy techniques; behavioral and physiological assays; and cytogenetic, biochemical and molecular biology techniques. This book is an essential resource for anyone involved in plant nematology needing to refer to a readily available methodology standard, including students of nematology and parasitology, university lecturers and researchers, diagnostic laboratories, and quarantine and advisory service personnel. It provides a much needed compendium of the spectrum of information needed to work with these microscopic organisms.

Muu info

Suitable for students and researchers of nematology and parasitology, as well as quarantine and advisory service personnel.
About the Editors xv
Contributors xvii
Preface xix
1 Sampling
1(11)
Loes J.M.F. den Nijs
Jon Pickup
Ralf-Udo Ehlers
1.1 Introduction: The Purpose of Sampling
1(1)
1.2 Sampling Strategies
1(4)
1.2.1 Diagnostic sampling
1(1)
1.2.2 Sampling for detection
2(1)
1.2.3 Sampling for density estimates
3(2)
1.3 Sampling in Relation to Phytosanitary Requirements
5(1)
1.4 Soil Sampling for Endoparasitic Nematodes
5(1)
1.5 Soil Sampling for Ectoparasitic Nematodes
6(1)
1.6 Sampling for Entomopathogenic Nematodes from Soil Samples: Isolation and Baiting Techniques
6(2)
1.6.1 Baiting method
6(2)
1.7 Examples of Sampling Protocols for Pine Wood Nematodes and Potato Cyst Nematodes
8(1)
1.7.1 Sampling for B. xylophilus
8(1)
1.7.2 Sampling for G. rostochiensis and G. pallida
9(1)
1.8 Sampling Tools
9(1)
1.9 Handling and Storage of Samples
10(1)
1.10 References
10(2)
2 Methods for Nematode Extraction
12(30)
Nicole Viaene
Johannes Kallmann
Leendert P. G. Molendijk
2.1 Introduction
12(2)
2.2 Centrifugal Flotation
14(4)
2.2.1 Materials
15(1)
2.2.2 Procedure
15(3)
2.3 Extraction of Cysts
18(9)
2.3.1 Baunacke method
18(1)
2.3.1.1 Materials
18(1)
2.3.1.2 Procedure
18(2)
2.3.2 Fenwick can
20(1)
2.3.2.1 Materials
20(1)
2.3.2.2 Procedure
21(1)
2.3.3 Schuiling centrifuge
21(1)
2.3.3.1 Materials
22(1)
2.3.3.2 Procedure
22(1)
2.3.4 Seinhorst elutriator
23(1)
2.3.4.1 Materials
23(1)
2.3.4.2 Procedure
24(1)
2.3.5 Wye washer
25(1)
2.3.5.1 Materials
26(1)
2.3.5.2 Procedure
26(1)
2.4 Extraction of Vermiform Nematodes from Soil
27(11)
2.4.1 Baermann method
27(1)
2.4.1.1 Materials
27(1)
2.4.1.2 Procedure
28(1)
2.4.2 Cobb decanting and sieving method and the Flegg-modified Cobb method
29(1)
2.4.2.1 Materials
29(1)
2.4.2.2 Procedure
29(2)
2.4.3 Two-flask method
31(1)
2.4.3.1 Materials
31(1)
2.4.3.2 Procedure
32(1)
2.4.4 Oostenbrink elutriator
33(1)
2.4.4.1 Materials
33(1)
2.4.4.2 Procedure
34(1)
2.4.5 Seinhorst elutriator
35(1)
2.4.5.1 Materials
36(1)
2.4.5.2 Procedure
36(2)
2.5 Extraction of Nematodes from Plant Parts
38(2)
2.5.1 Direct observation
38(1)
2.5.2 Incubation
39(1)
2.5.3 Baermann method and mistifier
39(1)
2.5.4 Maceration
39(1)
2.5.5 Eggs
39(1)
2.6 Acknowledgement
40(1)
2.7 References
40(2)
3 Estimating Numbers
42(18)
Johannes Kallmann
Matthias Daub
Wim Wesemael
3.1 Introduction
42(1)
3.2 Estimating Nematodes in Plant Tissue
43(2)
3.2.1 Staining nematodes inside plant tissues with acid fuchsin
43(2)
3.3 Estimating Nematodes in Liquids
45(4)
3.3.1 Maintaining a reproducible accuracy in aliquots
45(1)
3.3.2 Using optical methods to estimate numbers in liquids
45(2)
3.3.3 Applying digital image analysis to facilitate optical methods
47(1)
3.3.4 Counting nematodes using light absorbance
48(1)
3.3.5 Counting nematodes using quantitative PCR
49(1)
3.4 Estimating Numbers of Cysts
49(4)
3.4.1 Using extraction techniques with high efficiency to separate organic debris
50(1)
3.4.2 Using organic solvents to separate cysts from organic float
50(1)
3.4.3 Mechanical separation of cysts (Globodera spp.) from organic float
51(1)
3.4.4 Direct visual enumeration of cysts attached to roots using transparent boxes
51(1)
3.4.5 Counting females using fluorescence
51(2)
3.5 Estimating Eggs and Juveniles Isolated from Cysts
53(1)
3.5.1 Estimating content of cysts using a homogenizer
53(1)
3.5.2 Estimating content of individual cysts by preparing a squash mount
54(1)
3.6 Estimating Gall Index
54(2)
3.7 Estimating Number of Egg Masses
56(2)
3.7.1 Staining egg masses with red food colour
56(1)
3.7.2 Estimating egg numbers isolated from egg masses
57(1)
3.8 References
58(2)
4 Screening Plants for Resistance/Susceptibility to Plant-parasitic Nematodes
60(11)
Wim Wesemael
4.1 Introduction
60(1)
4.2 Selection of Nematode Population
60(1)
4.3 Collection and Preparation of Inoculum
61(2)
4.3.1 Inoculum of cyst nematodes
61(1)
4.3.2 Inoculum of root-knot nematodes
61(1)
4.3.3 Inoculum of root-lesion and burrowing nematodes
62(1)
4.3.4 Inoculum of stem and bulb, and foliar nematodes
62(1)
4.3.5 Inoculum of semi-endoparasitic and ectoparasitic nematodes
63(1)
4.4 Setting up Resistance Screening Assays
63(5)
4.4.1 Pot type and growing medium
63(2)
4.4.2 Inoculation
65(2)
4.4.3 Harvest and data acquisition
67(1)
4.5 Marker-assisted Selection
68(1)
4.6 References
68(3)
5 Handling, Fixing, Staining and Mounting Nematodes
71(17)
Jonathan D. Eisenback
David J. Hunt
5.1 Introduction
71(1)
5.2 Handling Nematodes
71(4)
5.2.1 Nematode picks
71(2)
5.2.2 Nematode picking technique
73(1)
5.2.3 Pipettes
73(1)
5.2.4 Forceps
73(1)
5.2.5 Aspirator
73(1)
5.2.6 Micro-chambers
73(2)
5.2.7 Useful dishes
75(1)
5.3 Killing and Fixing Techniques
75(3)
5.3.1 Permanent mounts
76(1)
5.3.1.1 Seinhorst slow method
77(1)
5.3.1.2 Ryss express method
77(1)
5.3.1.3 Simple evaporation method
77(1)
5.3.2 Fixing for SEM and TEM
78(1)
5.4 Processing Nematodes for Temporary and Permanent Mounts
78(2)
5.4.1 Temporary mounts
78(1)
5.4.1.1 Support rings
78(1)
5.4.1.2 Agar pads
78(1)
5.4.2 Slide making
79(1)
5.4.2.1 Glass wool supports or Ballotini beads
79(1)
5.4.2.2 Fingernail polish or paint rings
79(1)
5.4.2.3 Wax support rings
79(1)
5.5 Mounting en face Views and Cross-sections
80(1)
5.6 Staining Nematode Structures and Secretions
81(1)
5.6.1 Vital stains
82(1)
5.7 Staining Nematodes in Plant Material
82(3)
5.7.1 Staining endoparasitic nematodes in roots
82(1)
5.7.2 Staining egg masses of root-knot nematodes
83(1)
5.7.3 Histology staining
83(1)
5.7.4 Staining techniques
84(1)
5.8 References
85(3)
6 Culturing Techniques
88(44)
Rosa H. Manzanilla-Lopez
Ralf-Udo Ehlers
6.1 Introduction
88(1)
6.2 Collecting Nematodes to Start a Culture
88(1)
6.3 Axenization and Gnotobiology in in Vitro Cultures
89(1)
6.4 Cleaning and Decontamination of Plant-parasitic Nematodes
90(6)
6.4.1 Cleaning of nematodes in water
90(1)
6.4.2 Surface sterilization
91(1)
6.4.2.1 Inorganic disinfectants
91(1)
6.4.2.2 Surface sterilization and decontamination using agar
92(1)
6.4.2.3 Surface sterilization assisted by glass beads and glass fibre
92(1)
6.4.2.4 Surface sterilization using Pluronic F127 gel
93(1)
6.4.3 Antiseptics and disinfectants for Tylenchida and Aphelenchida
93(1)
6.4.3.1 Hibitane diacetate
93(1)
6.4.3.2 Cetavlon
93(1)
6.4.3.3 Hydrogen peroxide
93(1)
6.4.3.4 Antibiotics
94(1)
6.4.3.5 Mercury chloride
94(1)
6.4.3.6 Disinfectants and sterilants for Dorylaimida and Diphtherophorida
95(1)
6.4.4 Testing for contamination by microorganisms after surface sterilization
96(1)
6.5 Plant Tissue Culture
96(2)
6.5.1 Roots of whole seedlings on agar
96(1)
6.5.1.1 Tomato seedlings for in vitro culturing of Meloidogyne javanica
97(1)
6.5.1.2 Ditylenchus angustus cultured on axenic rice seedlings
98(1)
6.5.1.3 Xiphinema index cultured on tomato and fig seedlings
98(1)
6.6 Explant Culture
98(3)
6.6.1 Tomato root explants
99(1)
6.6.2 Maize root explants
100(1)
6.6.3 Cucumber excised roots
100(1)
6.6.4 Cyst nematodes on excised roots
100(1)
6.6.5 Leaf cultures
100(1)
6.6.6 Potato tuber, potato plug and storage root cultures
101(1)
6.7 Callus Tissue Culture
101(4)
6.7.1 Alfalfa callus
103(1)
6.7.2 Banana fruit callus
103(1)
6.7.3 Rice and wheat callus
104(1)
6.7.4 Groundnut callus
104(1)
6.7.5 Carrot callus
105(1)
6.8 Carrot Disc Culture
105(1)
6.9 In Vitro Banana Plantlets
106(1)
6.10 Gnotobiotic Culture of Whole Plants
107(2)
6.10.1 Seed pouches
108(1)
6.11 Genetically Engineered Plants
109(2)
6.12 Model Plants
111(2)
6.12.1 Arabidopsis thaliana
111(1)
6.12.2 Lotus japonicus
112(1)
6.13 Pot Cultures
113(3)
6.13.1 Pot cultures of Meloidogyne and Nacobbus
113(2)
6.13.2 Pot cultures of cyst nematodes
115(1)
6.13.3 Storage of viable cysts of Heterodera spp.
116(1)
6.14 Production of Entomopathogenic Nematodes
116(5)
6.14.1 In vivo culturing
116(1)
6.14.1.1 Production of the host insect, Galleria mellonella
116(1)
6.14.1.2 Counting dauer juveniles
117(1)
6.14.2 In vitro culturing
117(1)
6.14.2.1 Isolation of symbiotic bacteria and production of bacterial stock cultures
118(1)
6.14.2.2 Identification of contaminants and phase variants of symbiotic bacteria
118(1)
6.14.2.3 Production of bacteria-free nematodes
118(2)
6.14.3 Establishment of monoxenic cultures
120(1)
6.14.3.1 Monoxenic cultures for mass production
120(1)
6.14.4 Culture of single nematodes or pairs in hanging drops
120(1)
6.15 Cryopreservation of Nematodes
121(1)
6.15.1 Protocol for cryopreservation and revival of nematodes
121(1)
6.16 Acknowledgements
122(1)
6.17 References
122(10)
7 Measuring Nematodes and Preparation of Figures
132(20)
Weimin Ye
David J. Hunt
7.1 Introduction
132(1)
7.2 Calibration
133(1)
7.3 Direct Measurement
134(7)
7.3.1 Computer measuring
134(1)
7.3.2 Drawing apparatus
134(1)
7.3.3 Drawing nematodes for measurement
135(1)
7.3.4 Nematode measurement ruler
136(1)
7.3.5 Measurement criteria
137(4)
7.4 Processing Measurement Data and Preparing for Publication
141(9)
7.4.1 Cobb formula
144(2)
7.4.2 Preparing figures for publication
146(1)
7.4.2.1 Line drawings
146(1)
7.4.2.2 Greyscale
147(1)
7.4.2.3 Colour
147(1)
7.4.2.4 Combination artwork
147(1)
7.4.2.5 Labelling
147(1)
7.4.2.6 Encapsulated PostScript (EPS) files
148(1)
7.4.2.7 Layers
148(2)
7.4.2.8 Resolution and apparent image quality
150(1)
7.5 Acknowledgement
150(1)
7.6 References
151(1)
8 Electron Microscopy Techniques
152(25)
Vladimir V. Yushin
Myriam Claeys
Jan L.M. Leunissen
Julia K. Zograf
8.1 Transmission Electron Microscopy
152(16)
8.1.1 Before fixation
153(1)
8.1.2 Primary fixation
154(1)
8.1.3 Chemical fixation
154(1)
8.1.4 Infiltration and embedding: epoxy resins
155(1)
8.1.5 Specimen preparation: acrylic resins
155(1)
8.1.6 Cryopreparation methods for TEM
156(3)
8.1.7 High-pressure freezing (HPF)
159(3)
8.1.8 Self-pressurized rapid freezing
162(1)
8.1.9 Freeze substitution
163(1)
8.1.10 Preparation of sections for TEM observations
164(3)
8.1.11 Transmission electron microscopy
167(1)
8.1.12 Concluding remark on TEM applications
168(1)
8.2 Scanning Electron Microscopy
168(4)
8.2.1 Preparation of nematodes for SEM
168(1)
8.2.2 Fixation
169(1)
8.2.3 Drying
170(1)
8.2.3.1 Critical-point drying
170(1)
8.2.3.2 Air drying
170(1)
8.2.3.3 Acetone drying
171(1)
8.2.3.4 Freeze drying
171(1)
8.2.3.5 Freeze substitution
171(1)
8.2.3.6 Resin infiltration
171(1)
8.2.4 Glycerin embedded specimens
171(1)
8.2.5 Cryofracture
172(1)
8.2.6 Mounting and coating
172(1)
8.3 Acknowledgements
172(1)
8.4 References
172(5)
9 Behavioural and Physiological Assays
177(18)
Roland N. Perry
9.1 Introduction
177(1)
9.2 Attraction/Repulsion Plate Assays
177(2)
9.3 Pluronic Gel Assays
179(1)
9.4 Movement Assays
179(2)
9.4.1 Response to non-volatiles
179(1)
9.4.2 Response to volatiles
180(1)
9.5 Electrophysiology
181(1)
9.6 Stylet Activity
181(1)
9.7 Water Content Changes
181(1)
9.8 Oxygen Consumption Assays
182(1)
9.9 Collecting Female Sex Pheromone, Virgin Females and Males
183(1)
9.10 Viability Tests
183(6)
9.10.1 Staining
183(1)
9.10.1.1 Vital/non-vital stains
183(1)
9.10.1.2 Lipid reserves
184(1)
9.10.2 Hatching
185(1)
9.10.2.1 Standard hatching assays
185(2)
9.10.2.2 Root diffusates and other hatching agents
187(1)
9.10.2.3 Fluorescence assay
188(1)
9.10.2.4 Adenosine triphosphate assay
188(1)
9.10.2.5 Trehalose assay
189(1)
9.10.3 Other methods
189(1)
9.11 References
189(6)
10 Staining Chromosomes
195(11)
Jonathan D. Eisenback
10.1 Introduction
195(1)
10.2 Staining Chromosomes of Root-knot Females
195(6)
10.2.1 Propionic-orcein staining of root-knot females
195(1)
10.2.1.1 Preparation of propionic-orcein stain
195(1)
10.2.1.2 Selecting and obtaining nematode material for cytological study
196(1)
10.2.2 Preparation of smears to study oogonial divisions and early stages of maturation of oocytes
197(1)
10.2.2.1 Hydrolysis
198(1)
10.2.2.2 Fixation
198(1)
10.2.2.3 Staining
198(1)
10.2.2.4 Mounting
199(1)
10.2.2.5 Examination of temporary preparations
199(2)
10.2.3 Summary of procedures for staining Meloidogyne chromosomes with propionic-orcein
201(1)
10.3 Staining Chromosomes of Free-living Nematodes
201(1)
10.3.1 Propionic-orcein staining (Hechler, 1970)
201(1)
10.3.2 DAPI florescent nuclear staining (Hasegawa et al, 2006)
202(1)
10.4 Staining Chromosomes of Giant Cells
202(2)
10.4.1 Seed germination pouch cultures
202(1)
10.4.2 Glasshouse cultures
202(1)
10.4.3 Feulgen staining
203(1)
10.4.4 Observation of stained material
203(1)
10.5 References
204(2)
11 Isoelectric Focusing of Proteins
206(12)
Janete A. Brito
11.1 Introduction
206(1)
11.2 Isoelectric Focusing of Proteins for Diagnostics of Cyst Nematodes
207(2)
11.2.1 Sample preparation and loading
207(1)
11.2.2 Isoelectric focusing run
207(1)
11.2.3 Gel staining
208(1)
11.2.4 Interpretation of results
208(1)
11.3 Isoelectric Focusing of Isozymes for Diagnostics of Root-knot Nematodes
209(7)
11.3.1 Preparation of egg-laying females for isozyme extraction
209(1)
11.3.2 Gel preparation
210(1)
11.3.2.1 Resolving gel (8%); reagents and preparation
210(1)
11.3.2.2 Stacking gel (4%); reagents and preparation
211(1)
11.3.2.3 Electrode (running) buffer
211(1)
11.3.3 Running the gel
211(1)
11.3.4 Isozyme extraction and gel loading
212(1)
11.3.5 Electrophoresis
212(1)
11.3.6 Staining
212(1)
11.3.6.1 Staining solution for esterase
212(4)
11.3.6.2 Staining solution for malate dehydrogenase
216(1)
11.3.6.3 Staining solution for glutamate-oxaloacetate transaminase
216(1)
11.3.6.4 Staining solution for superoxide dismutase
216(1)
11.3.6.5 Fixative solution (20% ethanol and 10% glycerin)
216(1)
11.4 References
216(2)
12 Molecular Identification of Nematodes using Polymerase Chain Reaction (PCR)
218(22)
Sergei A. Subbotin
12.1 Introduction
218(2)
12.2 Preservation of Nematodes for Molecular Studies
220(1)
12.2.1 Dry preservation
220(1)
12.2.2 Ethanol (ethyl alcohol) preservation
220(1)
12.2.3 DESS preservation
220(1)
12.2.3.1 Protocol for preparing DESS solution
220(1)
12.3 DNA Extraction
221(1)
12.3.1 Protocols for DNA extraction from nematodes
221(1)
12.4 PCR Technique
222(9)
12.4.1 Protocols for PCR
230(1)
12.4.2 Gel electrophoresis
231(1)
12.5 PCR-Restriction Fragment Length Polymorphism
231(1)
12.6 Real-time PCR
232(2)
12.7 Problems with PCR-based Methods
234(1)
12.8 PCR Purification
234(1)
12.9 Cloning of PCR Products
235(1)
12.10 DNA Sequencing
235(1)
12.11 References
235(5)
13 Isolation and Characterization of Tandem Repeats in Nematode Genomes
240(10)
Philippe Castagnone-Sereno
13.1 Introduction
240(1)
13.2 Bench Strategies for the Identification of Tandem Repeats
241(3)
13.2.1 The case of satellite DNA
241(1)
13.2.2 The case of microsatellites
242(2)
13.3 In silico Identification of Tandem Repeats
244(1)
13.4 The Contribution of Tandem Repeats as Molecular Markers in Nematodes
245(1)
13.4.1 Satellite DNA-based species-specific diagnostic tools
245(1)
13.4.2 Microsatellites and population genetic studies in plant-parasitic nematodes
245(1)
13.5 Concluding Remarks
246(1)
13.6 References
246(4)
14 Characterization of Nematode Mitochondrial Genomes
250(15)
Danny A. Humphreys-Pereira
Taeho Kim
Joong-Ki Park
14.1 Introduction
250(2)
14.2 PCR Amplification of Mitochondrial Genomes
252(1)
14.3 Protocols for PCR
252(3)
14.3.1 Amplification of partial mt genes/short DNA fragments (< 3 kb)
252(1)
14.3.2 Long-range PCR
252(3)
14.4 Cloning and Sequencing
255(1)
14.5 Mitochondrial Genome Annotation and Gene Identification
255(6)
14.5.1 Protocols for mitochondrial genome annotation
256(5)
14.5.2 Important information after automated annotation
261(1)
14.6 References
261(4)
15 Phylogenetic Analysis of DNA Sequence Data
265(18)
Sergei A. Subbotin
15.1 Introduction
265(2)
15.2 Visualization and Editing of Raw Sequence Data
267(1)
15.3 Sequence Assembling
268(1)
15.4 Similarity Search in a Sequence Database
269(1)
15.5 Sequence Retrieval from the Database
270(3)
15.5.1 Multiple alignment
271(1)
15.5.2 Sequence alignment editing
271(2)
15.6 File Format Converting
273(1)
15.7 Selection of Model of Sequence Evolution
274(1)
15.7.1 jModelTest: A tool to select the best-fit model of nucleotide substitution
274(1)
15.8 Phylogenetic Analysis with PAUP*
275(3)
15.8.1 Distance method
276(1)
15.8.2 Maximum parsimony
276(1)
15.8.3 Maximum likelihood
277(1)
15.9 Phylogenetic Analysis with MrBayes
278(1)
15.10 Visualization of Phylogenetic Trees
279(2)
15.11 Sequence Submission
281(1)
15.12 References
281(2)
Index 283
Roland N Perry (Edited By) Professor Roland Perry is based at the University of Hertfordshire, UK. He graduated with a BSc (Hons) in Zoology from Newcastle University, UK, where he also obtained a PhD in Zoology on physiological aspects of desiccation survival of Ditylenchus spp. After a year's post doctoral research at Newcastle, he moved to Keele University, UK, where he taught Parasitology; after 3 years at Keele, he was appointed to Rothamsted Experimental Station (now Rothamsted Research). His research interests centred primarily on plant-parasitic nematodes, especially focusing on nematode hatching, sensory perception, behaviour and survival physiology, and several of his past PhD and post-doctoral students are currently involved in nematology research. He remained at Rothamsted until 2014, when he moved to the Department of Biological and Environmental Sciences, University of Hertfordshire. He co-edited The Physiology and Biochemistry of Free-living and Plant-parasitic Nematodes (1998), Root-knot Nematodes (2009), Molecular and Physiological Basis of Nematode Survival (2011), the first (2006) and second (2013) editions of the text book, Plant Nematology and Cyst Nematodes (2018) (all CAB International, UK). He is author or co-author of over 40 book chapters and refereed reviews and over 120 refereed research papers. He is joint Editor-in-Chief of Nematology and Chief Editor of the Russian Journal of Nematology. He is joint Editor of the book series Nematology Monographs and Perspectives. In 2001, he was elected Fellow of the Society of Nematologists (USA) in recognition of his research achievements; in 2008 he was elected Fellow of the European Society of Nematologists for outstanding contribution to the science of Nematology; and in 2011 he was elected Honorary Member of the Russian Society of Nematologists. He is a Visiting Professor at Ghent University, Belgium, where he lectures on nematode biology, focusing on physiology and behaviour.

David J Hunt (Edited By) David Hunt gained his B.Sc. at the University of Nottingham, UK, and his Ph.D. degree at the University of Reading, UK. From 1975 to 1979 he worked as a field nematologist in the West Indies and has travelled widely in the Caribbean region and in eastern and southern Africa. From 1979 until retiring in 2010 he worked in the UK as a nematode biosystematist for CABI, first at the International Institute of Parasitology and then at CABI Bioscience, where he is now an Emeritus Fellow. David has taught many international courses in nematode identification, these being mainly based in the UK, but also in Africa and Central America. During 2002-2022 he was joint Editor-in-Chief of the journal Nematology and joint Series Editor of the multi-volume series Nematology Monographs and Perspectives (Brill). He has published over 100 new nematode taxa, working mainly with parasites of diplopods and insects, contributed numerous chapters to a broad spectrum of books, authored the book Aphelenchida, Longidoridae and Trichodoridae: their systematics and bionomics and, with Khuong Nguyen, co-edited the monographs Entomopathogenic nematodes: systematics, phylogeny and bacterial symbionts and Advances in entomopathogenic nematode taxonomy and phylogeny.

Sergei A Subbotin (Edited By) Dr Sergei Subbotin obtained an MSc in Plant Protection in 1981 from the Moscow Agricultural Academy in Russia. In the same year, he joined the Plant Pathology Laboratory in the V. R. Williams All-Russian Fodder Research Institute, Moscow region, and later All-Russian Scientific Research Institute of Fundamental and Applied Parasitology of Animals and Plants named after K. I. Skryabin (former VIGIS), Moscow, where he was awarded a PhD in Helminthology (Nematology) in 1987. Dr Subbotin has worked as a researcher in the Center of Parasitology of the A. N. Severtsov Institute of Ecology and Evolution (Moscow, Russia), Rothamsted Experimental Station (now Rothamsted Research, Harpenden, UK), Institute for Agricultural and Fisheries Research (ILVO, Merelbeke, Belgium), Julius Ku¨hn-Institut (Mu¨nster, Germany), Department of Nematology, University of California (Riverside, USA) and as a Visiting Professor in Ghent University, Belgium. Presently, he serves as Senior Nematologist in the Plant Pest Diagnostic Center, California Department of Food and Agriculture, Sacramento, California, USA, and has affiliation positions as Scientist in the Center of Parasitology, Russia and Associate Scientist in the University of California, USA. Dr Subbotin is one of the Founders and Chief Editors of the Russian Journal of Nematology. He has published more than 190 scientific articles in peer-reviewed journals on different aspects of molecular systematics and diagnostics of nematodes, and is the author of 22 book chapters. He is co-author of the two volume Systematics of Cyst Nematodes (Nematoda: Heteroderinae) (2010) (Brill, The Netherlands), Systematics of the Sheath Nematodes of the Superfamily Hemicycliophoroidea (2014) (Brill) and Entomoparasitic Nematodes of the order Tylenchida and Aphelenchida (2019) (in Russian; KMK, Russia), and co-editor of the two volume Plant Parasitic Nematodes in Sustainable Agriculture of North America (2018) (Springer, Germany). His research studies have focused on molecular systematics, diagnostics and phylogeography of nematodes.