This volume covers a variety of the latest scientific methods utilized in tick-borne virus (TBV) research. The chapters in this book explore detailed protocols on how to conduct in-depth research on TBVs; enhance understanding of the virus’ biology; transmission mechanisms; and the creation of effective countermeasures. Following the established Methods in Molecular Biology series format, each chapter introduces the topic, lists necessary materials and reagents, offers step-by-step reproducible laboratory protocols, and includes troubleshooting tips to help avoid common issues.
Cutting-edge and thorough, Tick-Borne Viruses: Methods and Protocols is a comprehensive and innovative resource for researchers, clinicians, and public health experts interested in exploring the complexities of TBVs.
Tick Cell Culture Methods.- Virus Isolation and Culture from Field
Collected Ticks: In Vitro.- Virus Isolation from Field-Collected Ticks: In
Vivo.- Generation of Ticks Infected with Tick-Borne Encephalitis Virus.-
Generation of Virus Infected Ticks: Synchronous Infection and Feeding on
Viremic Animals.- Human Skin Ex-Vivo Culture for Tick-Borne Virus Infection
Studies.- Colorado Tick Fever Virus: Quantification, Propagation, Storage,
and Detection.- Isolation and Quantitation of Dabie bandavirus.-
Immunofluorescence Assays to Visualize Tick-Borne Flaviviruses in Mouse Skin
or Brain.- Immunohistochemical Assays to Visualize Tick-Borne Flaviviruses at
the Tick Feeding Site.- RNA In-Situ Hybridization to Visualize Tick-Borne
Viruses.- Quantification of Tick-Borne Flaviviruses Using Focus Forming
Assays.- Quantification of Neutralizing Antibodies to Tick-Borne Viruses
using Focus Neutralization Assays.- Methods for Evaluation of Antivirals and
Neutralizing Antibodies Against Tick-Borne Encephalitis Virus.- Exploring
Tick-Borne Encephalitis Virus-Host Cell Interactions using Electron
Tomography: Methodologies and Protocols.
