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Toxoplasma gondii: Methods and Protocols 2020 ed. [Pehme köide]

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  • Formaat: Paperback / softback, 470 pages, kõrgus x laius: 254x178 mm, kaal: 913 g, 63 Illustrations, color; 14 Illustrations, black and white; XI, 470 p. 77 illus., 63 illus. in color., 1 Paperback / softback
  • Sari: Methods in Molecular Biology 2071
  • Ilmumisaeg: 23-Nov-2020
  • Kirjastus: Humana Press Inc.
  • ISBN-10: 1493998595
  • ISBN-13: 9781493998593
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Toxoplasma gondii: Methods and Protocols 2020 ed.Suurem pilt
  • Formaat: Paperback / softback, 470 pages, kõrgus x laius: 254x178 mm, kaal: 913 g, 63 Illustrations, color; 14 Illustrations, black and white; XI, 470 p. 77 illus., 63 illus. in color., 1 Paperback / softback
  • Sari: Methods in Molecular Biology 2071
  • Ilmumisaeg: 23-Nov-2020
  • Kirjastus: Humana Press Inc.
  • ISBN-10: 1493998595
  • ISBN-13: 9781493998593
Teised raamatud teemal:
Püsilink: https://www.kriso.ee/db/9781493998593.html

This volume covers a diverse collection of protocols currently being used by the Toxoplasma research community, and also looks at innovative methods that are pushing the boundaries of possibilities. Chapters in this book discuss topics such as isolation and genotyping of Toxoplasma gondii strains; assessing rhoptry secretion in T. gondii; plate-based quantification of stimulated Toxoplasma egress; methods to study ocular toxoplasmosis; and metabolic analysis of Toxoplasma gondii tachyzoites. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Toxoplasma gondii: Methods and Protocols is a valuable resource for students or groups starting off in the field, as well as laboratories interested in implementing the latest techniques described in the book.


What a Difference 30 years Makes! A Perspective on Changes in research
Methodologies used to Study Toxoplasma gondii.- ToxoDB: Functional Genomics
Resource for Toxoplasma and Related Organisms.- Isolation and Genotyping of
Toxoplasma gondii Strains.- Generation of Toxoplasma gondii and Hammondia
hammondi Oocysts and Purification of their Sporozoites for Downstream
Manipulation.- Assay for Monitoring Toxoplasma gondii Infectivity in the
Laboratory Mouse.- PCR Screening of Toxoplasma gondii Single Clones Directly
from 96-Well Plantes without DNA Purification.- CRISPR/Cas9-Mediated
Generation of Tetracycline Repressor-Based Inducible Knock-Down in Toxoplasma
gondii.- Assessing Rhyptry Secretion in T. gondii.- High-Throughput
Measurement of Microneme Secretion in Toxoplasma gondii.- Plate-Based
Quantification of Stimulated Toxoplasma egress.- Genetic Indicators for
Calcium Signaling Studies in Toxoplasma gondii.- Phenotyping Toxoplasma
Invasive Skills by Fast Live Cell Imaging.- Experimental Approaches for
Examining Apicoplast Biology.- Measuring Solute Transport in Toxoplasma
gondii Parasites.- Toxoplasma gondii: Bradyzoite Differentiation In Vitro and
In Vivo.- Three-Dimensional Reconstruction of Toxoplasma-Neuron Interactions
In Situ.- Model Systems for Studying Mechanisms of Ocular
Toxoplasmosis.- Using BioID for the Identification of Interacting and
Proximal Proteins in Subcellular Compartments in Toxoplasma gondii.- Assays
to Evaluate Toxoplasma-Macrophage Interactions.- Methods for the Measurement
of Early Events in Toxoplasma gondii Immunity in Mouse Cells.- Image-based
Quantitation of Host Cell-Toxoplasma gondii Interplay using HRMan; A Host
Response to Microbe Analysis Pipeline.- Metabolomic Analysis of Toxoplasma
gondii Tachyzoites.- Label-Based Mass Spectrometry Approaches for Robust
Quantification of the Phospho- and Total Proteome in Toxoplasma gondii.