Novel molecular techniques, such as next-generation sequencing, are used to measure gene expression after exposure to a certain stimulus. Data using these gene expression techniques are highly accurate, sensitive and generate transcriptional profiles from species including humans, fish and crustaceans. This book includes transcriptomic studies of non-infectious and infectious diseases affecting humans and environmental and physiological correlates affecting shrimp and fish aquaculture. The book is intended for undergraduate and graduate students interested in one of the various research areas transformed by transcriptomics, including human disease, fish and crustacean physiological, environmental and farming issues.
Key Features
Documents the utility of next-generation sequencing and RNA-seq to a wide array of aquatic environmental and physiological issues as well as to human health
Provides insights into the ways transcriptomics can contribute to the understanding of various research subjects such as aquatic animals, fish ecology and human diseases
Presents an account of the evolution of the techniques used to determine the transcriptome in crustacean aquaculture
Describes the mechanisms of genetic interactions between different pathogens and the human host and their effects modifying gene expression levels
| Preface |
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vii | |
| Contributors |
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ix | |
| Introduction |
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1 | (4) |
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1 Shrimp Transcriptomics: Genome and Physiological Features |
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5 | (36) |
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Cesar Marcial Escobedo-Bonilla |
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2 Transcriptomics as a Mechanism to Study Crustacean Host-Pathogen Interactions |
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41 | (30) |
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Jose Reyes Gonzalez-Galaviz |
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Jesus Guadalupe Garcia-Clark |
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Cesar Marcial Escobedo-Bonilla |
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Libia Zulema Rodriguez-Anaya |
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3 Fish Transcriptomics: Applied to Our Understanding of Aquaculture |
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71 | (20) |
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4 Transcriptomics Applied in Research of Non-Communicable Diseases |
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91 | (34) |
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Ana Karen Gonzdlez-Palomo |
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Juan Carlos Ferndndez-Macias |
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Velia Veronica Rangel-Ramirez |
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Jorge Armando Jimenez-Avalos |
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5 The Impact of MicroRNAs on Human Diseases |
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125 | (20) |
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Jose Luis Acosta Rodriguez |
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6 Transcriptomics to Elucidate the Mechanisms of Pathogen-Human Interactions |
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145 | (32) |
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Libia Zulema Rodriguez-Anaya |
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| Index |
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177 | |
Dr. Libia Zulema Rodríguez-Anaya is a Doctor in Biotechnology Sciences from Instituto Tecnologico de Sonora, and a Lecturer of molecular biology, genetics and environmental microbiology since January 2016. She specializes in comparative analysis of complete virus genomes using next generation sequencing techniques. She has good command of bioinformatic tools for genome curation and molecular epidemiology studies. She is a CONACYT-Research Fellow, developing projects on structural and functional genomics applied to diagnoses and/or treatments for pathogenic free-living (FLA) organisms affecting humans. Dr Rodríguez-Anaya has authored and co-authored four peer-reviewed international papers on molecular characterization, pathogenicity and genomic mechanisms of viruses affecting shrimp and FLA-causing granulomatous amebic encephalitis and keratitis in humans.
Dr. César Marcial Escobedo Bonilla has a PhD in Veterinary Sciences from Ghent University, Belgium, and an MSc in Aquaculture and Environmental Management, CIAD Mazatlán. BSc Biology, UNAM ENEP Iztacala. He is a tenured researcher level C at the Aquaculture Department, IPN-CIIDIR Sinaloa, and a Member of the National Researchers System level I (Mexico). He has authored and co-authored twenty-four indexed papers, two book chapters on aquaculture and shrimp biology, and forty-two works in National and International congresses. He is a Lecturer at graduate level in courses related to aquaculture, and Theses director at bachelor level (2), MSc level (6) and PhD level (1). His research interests include infectious diseases of shrimp, bivalves, frogs and biocontrol of agricultural pests using viruses and entomopathogens. He is a project evaluator at national and international levels, a participant in committees evaluating books, and a reviewer of articles for international Journals listed in the Journal Citation Report and CONACyT. Dr. Bonilla is a member of scientific societies in aquaculture and virology. He won the 2008 prize for the most consulted paper in the Journal of Fish Diseases.
