Vector Borne Diseases, Volume 58 in the Methods in Microbiology series, highlights new advances in the field, with this new volume presenting interesting written by an international board of authors. Chapters in this new release cover Methods for Isolation and Characterization of Arthropod Hemocytes, Tick-to-Host Transmission of B. burgdorferi: In Vitro and In Vivo Methodologies, Decoding the Zika Virus outbreak and Infection Dynamics, Visual and molecular methods for studying pathogen persistence in flea vectors, Lipidomic Analysis of Virus-infected Cells, and Implementation of the Neutropenic Thigh Infection Murine Model with Francsiella tularensis for Development of New Antibacterials.
1. Methods for Isolation and Characterization of Arthropod Hemocytes
Deborah Anderson, Trevor Rodriguez and Caleb Kramer
2. TBA
Roman Reddy Ganta
3. TBA
Roger William Stich
4. Tick-to-Host Transmission of B. burgdorferi: In Vitro and In Vivo
Methodologies
Atieh Shemshadian, Nicholas J. Mantis and Daniel Palmer
5. Decoding the Zika Virus outbreak and Infection Dynamics
Iqra Bano
6. Visual and molecular methods for studying pathogen persistence in flea
vectors
Cassandra Dawn Pauling
7. Lipidomic Analysis of Virus-infected Cells
Eva Herker
8. TBD
Ram Raghavan
9. Implementation of the Neutropenic Thigh Infection Murine Model with
Francsiella tularensis for Development of New Antibacterials
Joel A CIV DHA USAMRIID Bozue, Kevin D. Mlynek and James Matthew CIV DHA
USAMRIID Meinig
Dr. Michael (Mick) Calcutt is a Professor and the Director of Graduate Studies for the Molecular Pathogenesis and Therapeutics Program at the Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri. He completed his BSc. in the University of Sheffield, UK, and his Ph.D in the University of Leicester, UK.
Dr. Calcutt's research focuses on aspects of the molecular genetics and pathogenesis of Mycoplasmas, a diverse genus of cell wall-less bacteria that includes many important pathogens of food animals and humans. In the absence of a cell wall, the single limiting membrane represents the unique and critical interface between the pathogen and host. Analysis of the repertoire, function and potential antigenic variation of surface membrane proteins is one of the primary research interests in the laboratory, together with understanding chromosomal dynamics and the role of mobile genetic elements in such plasticity. Increasingly, whole genome sequencing and comparative genomic approaches are being exploited to systematically assess the deduced surface proteome, horizontal gene transfer and accessory gene pool that likely contribute to the pathobiology of these organisms. Dr Gurtler completed a Ph.D. program in 1996 at La Trobe University in the Department of Microbiology. The main contribution of this work was the development of a universal bacterial typing technique based on the 16S-23S rDNA spacer region. The technique is now extensively used in many areas of Microbiology including Diagnostic, Environmental and Veterinary Microbiology. Citations to the articles Dr Gurtler wrote on this topic total >1000 with >830 citations alone to the 1996 review published in the journal Microbiology”. Dr Gurtler wrote an invited review on genomic typing, taxonomy and identification of bacterial isolates for the International Journal of Systematic and Evolutionary Microbiology (IJSEM). In addition to fundamental research, Dr Gurtler has also had experience with the application of this technology to the medical diagnostic laboratory in the fields of molecular microbiology and human molecular genetics. Dr Gurtler has been involved full time in diagnostic microbiology over many years resulting in the adoption of a method that identifies Mycobacterium species directly from clinical specimens without the need for culture in specimens positive for acid fast bacilli, the discovery of Nocardia veterana, and the development of many diagnostic tests.
In the last twelve years Dr Gurtler has been an Editor for the Journal of Microbiological Methods (JMM) and in the last 5 years Serial Editor of Methods in Microbiology (MIM) with the publication of 9 volumes covering diverse subjects such as Biofilms, Nanotechnology, COVID-19 and Fluorescent Probes.
