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West Nile Virus: Methods and Protocols 2023 ed. [Kõva köide]

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  • Formaat: Hardback, 228 pages, kõrgus x laius: 254x178 mm, kaal: 656 g, 28 Illustrations, color; 8 Illustrations, black and white; XI, 228 p. 36 illus., 28 illus. in color., 1 Hardback
  • Sari: Methods in Molecular Biology 2585
  • Ilmumisaeg: 05-Nov-2022
  • Kirjastus: Springer-Verlag New York Inc.
  • ISBN-10: 1071627597
  • ISBN-13: 9781071627594
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  • Formaat: Hardback, 228 pages, kõrgus x laius: 254x178 mm, kaal: 656 g, 28 Illustrations, color; 8 Illustrations, black and white; XI, 228 p. 36 illus., 28 illus. in color., 1 Hardback
  • Sari: Methods in Molecular Biology 2585
  • Ilmumisaeg: 05-Nov-2022
  • Kirjastus: Springer-Verlag New York Inc.
  • ISBN-10: 1071627597
  • ISBN-13: 9781071627594
Teised raamatud teemal:

This volume provides researchers with the most updated understanding of the basics of West Nile Virus (WNV). Chapters focus on the biology, virology, epidemiology, pathogenesis, as well as the step-by-step molecular, cellular, and statistical methods to study WNV infection in cell culture, mosquitos, animal models, and human clinical specimen.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, West Nile Virus: Methods and Protocols aims to be a valuable resource for all researchers interested in learning more about this important and developing field.

Introduction to West Nile Virus.- Quantification of West Nile virus by
Plaque-Forming Assay.- Viral titer quantification of West Nile virus by
immunostaining plaque assay.- Isolate and Culture Mouse Primary Neurons for
West Nile Virus Infection.- Isolation of Murine Bone Marrow Derived
Neutrophils for Infection Modeling.- Methods to study West Nile Virus
infection, and the virus-induced inflammation in the brain in a murine
model.- Detection of West Nile virus envelope protein in brain tissue with an
immunohistochemical assay.- Quantitative Analysis of B Cell Subpopulations in
Bone Marrow by Flow Cytometry.- Isolation of exosomes or extracellular
vesicles from West Nile Virus-infected N2a cells, Primary Cortical Neurons
and Brain Tissues.- Protocol to Study West Nile Virus Infection in Brain
Slices In Vitro.- Studying Virus-Host Interactions with CRISPR
technology.- Protocol of detection of West Nile Virus in Clinical
Samples.- Detection and Analysis of West Nile Virus Structural Protein Genes
in Animal Or Bird Samples.- Mosquito surveillance for West Nile
Virus.- Molecular surveillance of West Nile virus in mosquitoes and sentinel
chickens.- Statistical tools for West Nile Virus Disease
Analysis.- Analytical Approaches to Uncover Genetic Associations for Rare
Outcomes: Lessons from West Nile Neuroinvasive Disease.- Safety procedures to
work with West Nile virus in biosafety level 3 facilities.- Development of
antibody-based therapeutics against West Nile virus in plants.